您好, 歡迎來到化工儀器網(wǎng)! 登錄| 免費(fèi)注冊(cè)| 產(chǎn)品展廳| 收藏商鋪|
當(dāng)前位置:BPS Bioscience Inc.>>技術(shù)服務(wù)>>分子生物學(xué)>> BPS Bioscience的慢病毒定制化服務(wù)(英文)
參 考 價(jià) | 面議 |
產(chǎn)品型號(hào)
品 牌BPS Bioscience
廠商性質(zhì)生產(chǎn)商
所 在 地
更新時(shí)間:2023-12-19 23:27:43瀏覽次數(shù):247次
聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)BPS Bioscience的慢病毒定制化服務(wù)(英文)
慢病毒定制化服務(wù)
BPS Bioscience has developed a diverse portfolio of lentivirus products to study , cell signaling pathways, CAR T-cell therapy, CRISPR, and immunotherapy. In addition to our product portfolio, we also offer custom services to develop a lentivirus suited for your research needs.
Customizable Options
Reporters (GFP, Luciferase, Dual Reporter)
Antibiotic selection markers (Puromycin, Hygromycin, G418)
Integrating or non integrating
Pseudotyped
CRISPR lentiviruses
CRISPR activation lentiviruses
All products are supplied as ready-to-use viral particles. Depending upon the lentivirus, applications include
Stable cell line generation
Protein expression
Transient knock-down of protein in target cells
Study the mechanism of viral transduction
Screening for neutralizing antibodies
BPS’s lentiviruses are enveloped viruses that enter cells by membrane fusion. Our lentiviruses have been VSV-G pseudotyped; this means that the virus consists of vector particles bearing glycoproteins derived from other enveloped viruses—in this case, vesicular stomatitis virus GP (VSV-G). VSV-G pseudotyping provides stability and a very broad host range, making these virus particles especially useful to target a wide range of cell types.
Lentivirus Advantages
Infect both actively-dividing and non-dividing cells, whereas most viruses can only infect mitotically-active cells
Infect a wide range of cell stages
Ideal for transducing primary cells or stem cells, which can be resistant to other transduction methods
Size of inserted DNA can be up to 10 kb
Long-term stable expression of a transgene
Low immunogenicity, low toxicity, and transduction efficiencies can be extremely high (up to 99%)
In conjunction with our custom lentivirus services, we also offer lentivirus generated custom cell line development. These services can also be utilized for custom lentivirus generated knock-out cell lines.
BPS Bioscience is here to advance your research and drug discovery programs. Contact us to learn more and get started.
Integrating Lentiviruses: The integrating lentivirus integrates randomly into the cell’s genome to express both the Cas9 and sgRNA. Puromycin selection increases the knockout efficiency by forcing high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies also depend on the cell type and the gene of interest.
Non-Integrating Lentiviruses: The non-integrating lentivirus is made with a mutated Integrase, resulting in only transient expression of the Cas9 and LAG3-targeting sgRNA. While this may minimize potential off-targeting risks due to either prolonged expression or integration of the Cas9, puromycin selection should not be used for more than 48 hours post-transduction, which may lower knockout efficiency.
Luciferase activity in Jurkat cells transduced with Firefly Luciferase Lentivirus (G418, Hygromycin and Puromycin), #79692
如果您需要了解更多關(guān)于我們的服務(wù)的信息,請(qǐng)聯(lián)系info。
請(qǐng)輸入賬號(hào)
請(qǐng)輸入密碼
請(qǐng)輸驗(yàn)證碼
以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。
溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。