磺化Cy3.5(sulfo Cyanine 3.5; sulfo Cy3.5)是一種發(fā)紅色熒光的花青素類熒光染料?；腔疌y3.5是磺化修飾過的Cy3.5，水溶性好，它在顯影時(shí)可以用561nm或者594nm的激光束激發(fā)然后用 Texas Red? 或相似的濾片觀察，所以也是一種非常常用的標(biāo)記染料。磺化Cy3.5可以用來標(biāo)記蛋白，抗體，多肽,核酸分子，納米粒等。

磺化Cy3.5水溶性非常好，可在水相標(biāo)記反應(yīng)體系中直接使用，不需要有機(jī)溶劑助溶，特別適合標(biāo)記對有機(jī)溶劑敏感的生物分子。另外由于強(qiáng)水溶性加染料分子本身帶電荷，磺化Cy3.5標(biāo)記到生物分子表面后不會引發(fā)疏水性聚集，提高熒光標(biāo)記產(chǎn)物的穩(wěn)定性。

炔烴(alkyne)是常用生物標(biāo)記基團(tuán)。通過銅催化點(diǎn)擊化學(xué)反應(yīng)(Copper-catalyzed azide–alkyne cycloaddition, CuAAC)，炔烴可以和疊氮(azide)生成穩(wěn)定的生物鏈接(bioconjugate)。點(diǎn)擊化學(xué)反應(yīng)效率很高，可在水相中(也可有機(jī)相)快速完成，即便在低濃度(μM)下。點(diǎn)擊化學(xué)反應(yīng)技術(shù)發(fā)展很成熟，各種相關(guān)試劑商業(yè)易得，各種生物標(biāo)記應(yīng)用廣泛。

Cy3.5標(biāo)記后的產(chǎn)物Ex/Em是591nm/604nm。和它具有相似熒光特性的染料包括Texas Red?，Alexa Fluor 594, BODIPY? TR, DyLight 594等。

英文名稱：Sulfo-Cyanine3.5 alkyne

     Sulfo-CY3.5 alkyne

中文名稱：硫代-青色素3.5炔烴

     水溶性Cyanine3.5炔基

分子式：C41H40N3K3O13S4

分子量：1028.32
溶解性：易水溶，亦可溶于DMF, DMSO

質(zhì)量控制：純度>95%

儲存條件：在-20°C中24個(gè)月。

運(yùn) 輸：室溫下長達(dá)3周。避免長時(shí)間接觸光線。

結(jié)構(gòu)式：

我公司其它染料：

ROX NHS ester, pure 6- isomer

BDP FL NHS ester

BDP TMR NHS ester

Cyanine3 NHS ester

Cyanine3.5 NHS ester

Cyanine5 NHS ester

Cyanine5.5 NHS ester

Cyanine7 NHS ester

Cyanine7.5 NHS ester

Pyrenebutyric acid NHS ester

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 NHS ester

Sulfo-Cyanine7 NHS ester

Dye azides

BDP FL azide

Coumarin 343 azide

Cyanine3 azide

Cyanine3.5 azide

Cyanine5 azide

Cyanine5.5 azide

Cyanine7 azide

Cyanine7.5 azide

FAM azide, 5-isomer

FAM azide, 6-isomer

PEP azide

Perylene azide

Pyrene azide 1

Pyrene azide 2

Pyrene azide 3

R110 azide, 5- isomer

R110 azide, 6- isomer

ROX azide, 5- isomer

Sulfo-Cyanine3 azide

Sulfo-Cyanine5 azide

Sulfo-Cyanine7 azide

TAMRA azide, 5-isomer

Dye alkynes

1-Ethynyl pyrene

3-Ethynyl perylene

BDP FL alkyne

Cyanine3 alkyne

Cyanine5 alkyne

Cyanine5.5 alkyne

Cyanine7 alkyne

Cyanine7.5 alkyne

FAM alkyne, 5-isomer

FAM alkyne, 6-isomer

Sulfo-Cyanine3 alkyne

Sulfo-Cyanine5 alkyne

TAMRA alkyne, 5-isomer

Dye maleimides

BDP FL maleimide

Cyanine3 maleimide

Cyanine5 maleimide

Cyanine5.5 maleimide

Cyanine7 maleimide

Cyanine7.5 maleimide

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine5 maleimide

Dye hydrazides

Cyanine3 hydrazide

Cyanine5 Boc-hydrazide

Cyanine5 hydrazide

Cyanine5.5 hydrazide

Cyanine7 hydrazide

Cyanine7.5 hydrazide

Carboxylic acids

Cyanine3 carboxylic acid

Cyanine3.5 carboxylic acid

Cyanine5 carboxylic acid

Cyanine5.5 carboxylic acid

Cyanine7 carboxylic acid

Cyanine7.5 carboxylic acid

Pyrenebutyric acid

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine5 carboxylic acid

Sulfo-Cyanine7 carboxylic acid

Amino dyes

Cyanine3 amine

Cyanine5 amine

Cyanine5.5 amine

Cyanine7 amine

Cyanine7.5 amine

Sulfo-Cyanine7 amine