目錄:北京北納創(chuàng)聯(lián)生物技術(shù)研究院>>ATCC>>ATCC細(xì)胞>> ATCCCCL-139TCMK-1 ATCC細(xì)胞購買
| 參考價(jià) | 面議 |
| 參考價(jià) | 面議 |
更新時(shí)間：2024-11-09 21:02:00瀏覽次數(shù)：2021評(píng)價(jià)
聯(lián)系我們時(shí)請(qǐng)說明是化工儀器網(wǎng)上看到的信息，謝謝!
公司介紹
北京北納創(chuàng)聯(lián)生物技術(shù)研究院是一家專業(yè)從事標(biāo)準(zhǔn)物質(zhì)、計(jì)量分析儀器和精細(xì)化工產(chǎn)品研發(fā)及銷售的科研單位。是國(guó)內(nèi)標(biāo)準(zhǔn)物質(zhì)提供商之一，代理銷售國(guó)內(nèi)外數(shù)萬種標(biāo)準(zhǔn)物質(zhì)、微生物、菌種、細(xì)胞類等產(chǎn)品。
北京標(biāo)準(zhǔn)物質(zhì)網(wǎng)隸屬于北京北納創(chuàng)聯(lián)生物技術(shù)研究院，具備強(qiáng)大的分析測(cè)試、技術(shù)開發(fā)、*、物流管理和售后服務(wù)能力，并與國(guó)內(nèi)外眾多檢測(cè)機(jī)構(gòu)、科研院校和工廠企業(yè)有著良好和穩(wěn)定的合作關(guān)系，具有較強(qiáng)的性和影響力。
北納生物也隸屬于北京北納創(chuàng)聯(lián)生物技術(shù)研究院，實(shí)驗(yàn)基地總占地面積16畝，并設(shè)有細(xì)胞庫、微生物菌種保藏庫，細(xì)胞培養(yǎng)、菌種培養(yǎng)等實(shí)驗(yàn)室。擁有細(xì)胞和菌株2萬余株，并設(shè)有生物資源信息庫，收錄信息達(dá)20余萬株。致力于提供實(shí)驗(yàn)室綜合解決方案，為廣大客戶服務(wù)。
經(jīng)營(yíng)范圍：
國(guó)家標(biāo)準(zhǔn)物質(zhì)、國(guó)內(nèi)外標(biāo)準(zhǔn)品、對(duì)照品、實(shí)驗(yàn)耗材、試劑、菌株、細(xì)胞、ATCC、培養(yǎng)基、血清、冶金標(biāo)樣、標(biāo)準(zhǔn)氣體、試劑盒等，歡迎查詢！
ATCCCCL-139 TCMK-1 ATCC細(xì)胞購買
| 基本信息 | |
| 資源編號(hào) | ATCCCCL-139 |
| 資源名稱 | TCMK-1 |
| 種屬 | Mus musculus, mouse kidney |
| 類型 | SV40 transformed |
| 形態(tài) | epithelial |
| 提供形式 | frozen |
| 致病類型 | normal |
| 安全等級(jí) | 2 |
| 培養(yǎng)方法 | |
| 培養(yǎng)基 | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| 傳代方法 | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| 生長(zhǎng)條件 | Temperature: 37°C |
| 生長(zhǎng)特性 | adherent |
| 存儲(chǔ)條件 | liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| 詳細(xì)說明 | |
| 描述 | About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox). |
| Age | weanling |
| Strain | C3H/Mai |
| Karyotype | modal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes. |
| Derivation | The cells are SV40 transformed and are positive for SV40 T-antigen. |
| Antigen Expression | H-2k |
| Tumorigenic | No |
| Effects | No, in immunosuppressed mice Yes, in semisolid medium |
| Virus Susceptibility | Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1 |
| Virus Resistance | poliovirus 1 |
| Year of Origin | 1963 |
ATCCCCL-139 TCMK-1 ATCC細(xì)胞購買
溫馨提示：
本店商品種類眾多，因鋪位有限商品未全部上架，如果沒有找到您想要的商品可以咨詢客服，我們會(huì)耐心解答，祝您購物愉快！
(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)
會(huì)員.png)
7
化工儀器網(wǎng)