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Cellartis定向分化獲取內(nèi)胚層細(xì)胞試劑盒

產(chǎn)品英文名稱：Cellartis Definitive Endoderm Differentiation Kit

產(chǎn)品中文名稱：Cellartis定向分化獲取內(nèi)胚層細(xì)胞試劑盒

用于定向分化hiPS獲取內(nèi)胚層細(xì)胞

The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS獲取definitive endoderm (DE) cells。本產(chǎn)品包含所有必需的培養(yǎng)基和coating材料。本產(chǎn)品還包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的維持培養(yǎng)用。使用本產(chǎn)品獲得的DE細(xì)胞，可以作為起始材料進(jìn)行定向分化至內(nèi)胚層來源的細(xì)胞類型(如肝細(xì)胞，胰腺細(xì)胞)。Cellartis也提供分化DE細(xì)胞獲取肝細(xì)胞的試劑盒（Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)；Cellartis也提供誘導(dǎo)分化hiPS獲取肝細(xì)胞的試劑盒（Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。

The Cellartis with DEF-CS Culture System contains a complete media set and a ready-to-use coating for the differentiation of human induced pluripotent stem (hiPS) cells into definitive endoderm (DE) cells in monolayer culture. The kit includes the Cellartis DEF-CS 100 Culture System for the maintenance of undifferentiated hiPS cells prior to DE cell differentiation. The DE cells obtained with this differentiation kit can be used for further differentiation into cells of endodermal origin, such as hepatocytes and pancreatic endoderm cells. For further differentiation of DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050); alternay, start directly from iPS cells using the complete Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055).

產(chǎn)品特點(diǎn):

有效的分化 - 產(chǎn)生> 80％的SOX17陽性細(xì)胞

強(qiáng)大的培養(yǎng)方法 - 來自超過25種不同hiPS細(xì)胞系成功分化為定形內(nèi)胚層（DE）細(xì)胞

完整的試劑盒 - 包括培養(yǎng)基和即用型包被，將hiPS細(xì)胞分化為DE細(xì)胞的實(shí)驗(yàn)方案為期七天，具備可重復(fù)性

起始為均質(zhì)的hiPS細(xì)胞群 - 試劑盒包括Cellartis DEF-CS 100培養(yǎng)系統(tǒng)以在DE細(xì)胞分化開始之前維持細(xì)胞的未分化狀態(tài)

Efficient differentiation—generates >80% SOX17-positive cells

Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines

Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells

Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation

組份：iPS細(xì)胞培養(yǎng)完整試劑盒+分化內(nèi)胚層試劑盒

Cellartis with DEF-CS Culture System (Cat. No. Y30035):

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020; not sold separay. A larger 500 ml version of this product is sold as Cat. No. Y30010.)

? 100 ml DEF-CS 100 Basal Medium

? 800 µl DEF-CS COAT-1 (for 100 ml)

? 300 µl DEF-CS GF-1 (for 100 ml)

? 100 µl DEF-CS GF-2 (for 100 ml)

? 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis (Cat. No. Y30030; not sold separay)

? 1 tube Definitive Endoderm Differentiation Coating (10 ml)

? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

自備試劑：（可選）

人多能干細(xì)胞

PBS Dulbecco's含有Ca2 +和Mg2 +（D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE選擇酶（1X），不含酚紅

保存：Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1   2–8°C；GF-1, GF-2, and GF-3  –20°C

Cellartis (Y30030)：–20°C

產(chǎn)品性能：

mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.

相關(guān)產(chǎn)品:

Cellartis Human ES Cell Line 121 (SA121) Kit (Cat No. Y00025)

Cellartis Human ES Cell Line 167 (SA167) Kit (Cat. No. Y00065)

Cellartis Human ES Cell Line 181 (SA181) Kit (Cat. No. Y00105)

Cellartis Human ES Cell Line 461 (SA461) Kit (Cat. No. Y00145)

Cellartis Human iPS Cell Line 7 (ChiPSC7) Kit (Cat. No. Y00275)

Cellartis Human iPS Cell Line 12 (ChiPSC12) Kit (Cat. No. Y00285)

Cellartis Human iPS Cell Line 18 (ChiPSC18) Kit (Cat. No. Y00305)

Cellartis Human iPS Cell Line 22 (ChiPSC22) Kit (Cat. No. Y00325)

Cellartis® Hepatocyte Differentiation Kit (Cat. No. Y30050)

Cellartis® iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)

肝細(xì)胞培養(yǎng)完整解決方案

人iPS誘導(dǎo)分化成肝細(xì)胞全過程的完整試劑盒：Y30055（新產(chǎn)品）

人iPS來源肝細(xì)胞株及*培養(yǎng)基Y10133/Y10134/Y10135（新產(chǎn)品）

人原代肝臟細(xì)胞培養(yǎng)基 Y20020（新產(chǎn)品）

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華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

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