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多能細(xì)胞檢測用單克隆抗體

產(chǎn)品英文名稱：Monoclonal Antibodies for Detecting Pluripotent Cells

產(chǎn)品中文名稱：Cellect系列多能細(xì)胞檢測用單克隆抗體

用途：鑒別、分析、分離具有發(fā)育多能性的人類胚胎干細(xì)胞（ES）和誘導(dǎo)多能干細(xì)胞（iPS）。

hES-Cellect 識(shí)別人多能細(xì)胞

ES-Cellect  識(shí)別人和鼠多能細(xì)胞

hFF-Cellect 識(shí)別人成纖維細(xì)胞

Y20010 hES-Cellect™ 150 μg

Y20011 ES-Cellect™ 150 μg

Y20012 hFF-Cellect™ 150 μg

Cellect系列單克隆抗體用于鑒別、分析、分離具有發(fā)育多能性的人類胚胎干細(xì)胞（ES）和誘導(dǎo)多能干細(xì)胞（iPS）。本系列抗體特異性地識(shí)別具有發(fā)育多能性的干細(xì)胞表面的抗原，而這些抗原在干細(xì)胞定向分化后會(huì)被弱化調(diào)控。

產(chǎn)品特點(diǎn)

hES-Cellect和ES-Cellect是識(shí)別多能細(xì)胞特異性表面標(biāo)記的高純度單克隆抗體

hFF-Cellect抗體識(shí)別人成纖維細(xì)胞，并具有比SSEA和TRA抗體更低的交叉反應(yīng)性

這些抗體使用時(shí)不需要細(xì)胞固定或破膜來檢測和選擇活細(xì)胞

hES-Cellect and ES-Cellect are high-purity monoclonal antibodies that recognize surface markers specific to pluripotent cells

The hFF-Cellect antibody recognizes human fibroblasts and has lower cross reactivity than SSEA and TRA antibodies that recognize fibroblasts and other human somatic cells

These antibodies can be used without cell fixation or permeabilization to detect and select live cells 

產(chǎn)品參數(shù)

成分：150 μg親和純化鼠單克隆抗體

同種型isotype：IgG1

免疫原：

特異性：

hES-Cellect 識(shí)別人ES胚胎干細(xì)胞和iPS誘導(dǎo)多能干細(xì)胞，hFF、mEF和mES陰性

ES-Cellect  識(shí)別人ES胚胎干細(xì)胞和iPS誘導(dǎo)多能干細(xì)胞及鼠ES胚胎干細(xì)胞，hFF、mEF陰性

hFF-Cellect 識(shí)別人FF成纖維細(xì)胞，hES和hiPS陰性。

hES細(xì)胞：human embryonic stem，人胚胎干細(xì)胞； hFF細(xì)胞：human foreskin fibroblast (hFF)，人皮膚成纖維細(xì)胞 。mEF細(xì)胞：mouse Embryonic Feeder 鼠胚胎飼養(yǎng)細(xì)胞；mES細(xì)胞： mouse Embryonic Stem 鼠胚胎干細(xì)胞

產(chǎn)品應(yīng)用

通過免疫細(xì)胞化學(xué)，流式細(xì)胞術(shù)和細(xì)胞磁珠分選鑒定和/或分離人ES和iPS細(xì)胞

從飼養(yǎng)細(xì)胞和分化后代分離人多能干細(xì)胞

重編程人類iPS細(xì)胞克隆的早期鑒定

染色表現(xiàn)

hES-Cellect

hiPSC on mEF, stained with hES-Cellect™ (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).

Staining performed according to protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Bright field, [b] Alexa Fluor 594, [c] DAPI.

ES-Cellect

hESC cultured on mEF, stained with ES-Cellect (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).

Staining performed according to the protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Bright field, [b] Alexa Fluor 594, [c] DAPI.

hFF-Cellect

hFF stained with hFF-Cellect™ (1 µg/ml) and goat anti-mouse IgG Alexa Fluor 594 (2 µg/ml).

Staining performed according to the protocol above. Pictures are shown in 20x magnification; scale bar corresponds to 50 µm. [a] Alexa Fluor 594, [b] DAPI, [c] overlapping.

訂購信息

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

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