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美國kernel禽傳染性喉氣管炎抗體檢測試劑盒 

規(guī)格 2*96,5*96,10*96 

雞傳染性喉氣管炎(Avian Infectious Laryngotracheitis， AILT)是由傳染性喉氣管炎病毒引起的一種急性、接觸性上部呼吸道傳染病。其特征是呼吸困難、咳嗽和咳出含有血樣的滲出物。剖檢時可見喉部、氣管粘膜腫脹、出血和糜爛。在病的早期患部細胞可形成核內包涵體。本病1925年在美國*報道后，現(xiàn)已遍及世界許多養(yǎng)雞地區(qū)。本病傳播快，死亡率較高，在我國較多地區(qū)發(fā)生和流行，危害養(yǎng)雞業(yè)的發(fā)展。 

Infectious laryngotracheitis (commonly known as ILT) is a viral respiratory disease of chickens centred on the trachea. ILT can cause serious production losses due to a combination of mortality and depressed egg production. This disease can manifest itself in a variety of forms ranging from severe to mild with the latter being increasingly encountered in developed poultry industries and appearing as a mucoid tracheitis, sinusitis and conjunctivitis coupled to unthriftiness and low mortality. 

INTENDED USE 
ELI-LTI ELISA kit is used for quantitative detection of antibodies against Infectious Laryngotracheitis virus (ILT) in chicken serum and yolk. 
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-ILT antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the ILT antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.