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D1045 5-Hydroxymethyl dCTP
品牌：ZYMO Research
代理商：慧穎生物
庫存：貨期產(chǎn)品
D1045 5-Hydroxymethyl dCTP
Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.
Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.
About Hydroxy/Methylated Nucleotides
5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
| For 5-hmC | For 5-mC | |||||||||
| Reagent | Volume | Final Concentration | Volume | Final Concentration | ||||||
| 2X Reaction Buffer | 25 µl | 1X | 25 µl | 1X | ||||||
| dNTP Mix | 0.5 µl | 0.25 mM each dNTP | 0.5 µl | 0.25 mM each dNTP | ||||||
| Forward Primer (10 µM) | Variable | 0.3 to 1 µM | Variable | 0.3 to 1 µM | ||||||
| Reverse Primer (10 µM) | Variable | 0.3 to 1 µM | Variable | 0.3 to 1 µM | ||||||
| Template DNA | Variable | < 200 ng/50 µl | Variable | < 200 ng/50 µl | ||||||
| ZymoTaq™ DNA Polymerase (5 U/µl) | 1 µl | 5 U/50 µl | 0.4 µl | <2 U/50 µl | ||||||
| ddH20 | to 50 µl | - | to 50 µl | - | ||||||
| Total Volume | 50 µl | 50 µl | ||||||||
| Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations. Specifications
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