新霉素ELISA快速檢測試劑盒(檢組織/乳制品，靈敏度0.5ppb，一步法)

                         新霉素ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預(yù)包被偶聯(lián)抗原，樣本中的殘留物新霉素將和微孔條上預(yù)包被的偶聯(lián)抗原競爭抗新霉素抗體，加入酶標(biāo)記物后，用TMB底物顯色，樣本吸光值與其所含殘留物新霉素的含量成負相關(guān)，與標(biāo)準曲線比較即可得出相應(yīng)殘留物新霉素的含量。

二、試劑盒特性

u  試劑盒靈敏度：   0.5ppb

u  孵育溫度：       25℃

u  孵育時間：       60min～15min

u  樣本檢測下限

組織（雞肉/肝、豬肉/肝、蝦、魚 ·········  10ppb

蜂    蜜 ·········································  10ppb

牛奶、奶粉·······································  10ppb

u  交叉反應(yīng)率

新霉素  ·········································   100%

鏈霉素  ·········································  ﹤0.1%

慶大霉素  ······································  ﹤0.1%

雙氫鏈霉素  ···································  ﹤0.1%

卡那霉素  ······································  ﹤0.1%

妥布霉素  ······································  ﹤0.1%

紫蘇霉素  ······································  ﹤0.1%

u  樣本回收率

組織（雞/豬肉、蜂蜜、蝦、魚）··········  85±19%

雞肝、豬肝  ···································  70±17%

牛奶、奶粉  ···································  85±16%

三、試劑盒組成

四、樣品前處理及檢測步驟

    詳見產(chǎn)品說明書。

五、儲藏條件和保質(zhì)期

1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。

2、 保 質(zhì) 期：該產(chǎn)品有效期為1年，生產(chǎn)日期見包裝盒。

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