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Genebridege公司的FLPe表達(dá)質(zhì)粒707-710可以促成大腸桿菌中以FLP為媒介的定點(diǎn)重組，在30°C時(shí)以低拷貝數(shù)傳播。Flpe基因由λR啟動(dòng)子驅(qū)動(dòng)，受控于不耐熱cI857阻遏蛋白。溫度從30°C上升至37°C會(huì)引發(fā)FLPe重組酶暫態(tài)表達(dá)。由于pSC101復(fù)制原點(diǎn)的緣故，表達(dá)質(zhì)粒在37°C時(shí)不再復(fù)制，因此它們zui終會(huì)消失。與野生型FLP蛋白相比，F(xiàn)LPe擁有更好的耐熱性，在37 – 40°C時(shí)顯示出更強(qiáng)的重組酶活性
產(chǎn)品信息如下：
All Red/ET Recombination Kits come with tailored control experiments and harbor the same Red/ET proficiency plasmid.
Quick & Easy BAC Modification Kit
| For all types of BAC modifications. | ||||
| ID: K001 | 690.00 €* | Details | Manual | Order |
Counter Selection BAC Modification Kit
| For advanced BAC modifications using a counter-selection cassette, also for bacterial chromosomes. | ||||
| ID: K002 | 795.00 €* | Details | Manual | Order |
BAC Subcloning Kit
| For subcloning of DNA fragments from BACs and cosmids. | ||||
| ID: K003 | 620.00 €* | Details | Manual | Order |
Quick & Easy Conditional Knock Out Kit - FRT
| For fast and simple integration of FRT-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）. | ||||
| ID: K004 | 690.00 €* | Details | Manual | Order |
Quick & Easy Conditional Knock Out Kit - loxP
| For fast and simple integration of loxP-sites into large vector plasmids at any intended position （for generation of transgenic mouse models）. | ||||
| ID: K005 | 690.00 €* | Details | Manual | Order |
Quick & Easy E.coli Gene Deletion Kit
| For fast knockouts or modifications of genes on the E.coli chromosome. | ||||
| ID: K006 | 620.00 €* | Details | Manual | Order |
Red/ET Recombination Strains
E. coli strain GB05-dir
| Direct cloning-proficient E. coli strain for linear plus linear homologous recombination | ||||
| ID: K008 | 590.00 €* | Details | Manual | Order |
E. coli strain GB08-red
| Recombineering-proficient E. coli strain for linear plus circular homologous recombination | ||||
| ID: K009 | 590.00 €* | Details | Manual | Order |
Selection Cassettes
Also available are accessory selection cassettes designed for use in Eukaryotes or Prokaryotes, some flanked by loxP- or FRT-sites. These sites will allow the subsequent removal of the selection marker by a Cre- or FLP-recombinase step if required. These cassettes may be very helpful for generating targeting constructs in mammals, or for introducing sequences without leaving a selection marker behind.
PGK-gb2-neo
| Pro- and Eukaryotic Neomycin Selection Cassette | ||||
| ID: A001 | 290.00 €* | Details | Manual | Order |
FRT-PGK-gb2-neo-FRT
| Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites | ||||
| ID: A002 | 290.00 €* | Details | Manual | Order |
loxP-PGK-gb2-neo-loxP
| Pro- and Eukaryotic Neomycin Selection Cassette flanked by loxP-sites | ||||
| ID: A003 | 290.00 €* | Details | Manual | Order |
FRT-PGK-gb2-neo-FRT-loxP
| Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional downstream loxP site | ||||
| ID: A004 | 290.00 €* | Details | Manual | Order |
loxP-FRT-PGK-gb2-neo-FRT
| Pro- and Eukaryotic Neomycin Selection Cassette flanked by FRT-sites and one additional upstream loxP site | ||||
| ID: A005 | 290.00 €* | Details | Manual | Order |
FRT-gb2-cm-FRT
| Prokaryotic Chloramphenicol Selection Cassette flanked by FRT-sites | ||||
| ID: A006 | 290.00 €* | Details | Manual | Order |
loxP-gb2-cm-loxP
| Prokaryotic Chloramphenicol Selection Cassette flanked by loxP-sites | ||||
| ID: A007 | 290.00 €* | Details | Manual | Order |
FRT-gb2-amp-FRT
| Prokaryotic Ampicillin Selection Cassette flanked by FRT-sites | ||||
| ID: A008 | 290.00 €* | Details | Manual | Order |
loxP-gb2-amp-loxP
| Prokaryotic Ampicillin Selection Cassette flanked by loxP-sites | ||||
| ID: A009 | 290.00 €* | Details | Manual | Order |
FRT-PGK-gb2-hygro-FRT
| FRT flanked, Pro- and Eukaryotic Hygromycin Selection Cassette | ||||
| ID: A010 | 290.00 €* | Details | Manual | Order |
loxP-PGK-gb2-hygro-loxP
| loxP flanked, Pro- and Eukaryotic Hygromycin Selection Cassette | ||||
| ID: A011 | 290.00 €* | Details | Manual | Order |
iCre-FRT-neo-FRT
| Codon improved Cre （iCre） with attached FRT flanked, Pro- and Eukaryotic Neomycin Selection Cassette | ||||
| ID: A012 | 325.00 €* | Details | Manual | Order |
Expression Plasmids
The following recombinase expression plasmids are very useful to excise FRT- or loxP-flanked DNA stretches （such as selection markers） during a modification task. The plasmids are easily transformed into cells resulting in an activation of the recombinases in vivo.
707-FLPe, tet
| Flp Expression Plasmid with tetracycline resistance marker for use in E.coli only | ||||
| ID: A104 | 290.00 €* | Details | Manual | Order |
708-FLPe, cm
| Flp Expression Plasmid with chloramphenicol resistance marker for use in E.coli only | ||||
| ID: A105 | 290.00 €* | Details | Manual | Order |
709-FLPe, ap
| Flp Expression Plasmid with ampicillin resistance marker for use in E.coli only | ||||
| ID: A106 | 275.00 €* | Details | Manual | Order |
710-FLPe, km
| Flp Expression Plasmid with kanamycin resistance marker for use in E.coli only | ||||
| ID: A107 | 275.00 €* | Details | Manual | Order |
705-Cre;cm
| Cre Expression Plasmid with chloramphenicol resistance marker for use in E.coli only | ||||
| ID: A112 | 290.00 €* | Details | Manual | Order |
706-Cre;tet
| Cre Expression Plasmid with tetracycline resistance marker for use in E.coli only | ||||
| ID: A113 | 290.00 €* | Details | Manual | Order |
pCAGGS-FLPe
| Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Academia）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement. | ||||
| ID: A201 | 375.00 €* | Details | Manual | Order |
pCAGGS-FLPe
| Enhanced Eukaryotic FLP Expression Plasmid optimized for 37°C （Industry）. The use of the this product is governed by the terms and conditions of the pCAGGS-FLPe Material Transfer Agreement. | ||||
| ID: A202 | 3250.00 €* | Details | Manual | Order |
