的PBS選擇-PBS片劑

時(shí)間：2017-3-27閱讀：466

產(chǎn)品說(shuō)明：

PBS（phosphate buffered solution）即磷酸鹽緩沖液，能夠提供相對(duì)穩(wěn)定的離子環(huán)境和pH緩沖能力，是生物學(xué)中經(jīng)常使用的緩沖鹽溶液，用于分子克隆及細(xì)胞培養(yǎng)等，pH為7.2-7.4，與人體血液等滲，主要成分為磷酸二氫鉀、磷酸氫二鈉、NaCl)，KCl)，。

PBS是磷酸緩沖鹽溶液（phosphate buffer saline）一般作為溶劑，起溶解保護(hù)試劑的作用。它是生物化學(xué)研究中使用的一種緩沖液，主要成分為Na2HPO4、KH2PO4、NaCl和KCl，由于Na2HPO4和KH2PO4它們有二級(jí)解離，緩沖的pH值范圍很廣；而NaCl和KCl主要作用為增加鹽離子濃度。如有需要PBS還可以補(bǔ)加1 mmol/L CaCl2和0.5 mmol/L MgCl2，以提供雙價(jià)陽(yáng)離子。

配制方法

含0.05%吐溫－20的pH7.4的磷酸鹽緩沖液(簡(jiǎn)稱(chēng)為PBS－T)配制方法為：

稱(chēng)取磷酸二氫鉀(KH2PO4)，磷酸氫二鈉(Na2HPO4·12H2O)，NaCl)，KCl)，吐溫－20 ，加水。

PBS:Phosphate Buffered Saline

PBS 1L配方pH7.4：磷酸二氫鉀(KH2PO4)：0.27g，

磷酸氫二鈉(Na2HPO4)：1.42g，

NaCl)：8g，

KCl)：0.2g，

加去離子水約800mL充分?jǐn)嚢枞芙猓缓蠹尤霛恹}酸調(diào)pH至7.4，zui后定容到1L

大部分實(shí)驗(yàn)室還是自己配制，不僅麻煩而且耗時(shí)，還有些實(shí)驗(yàn)室購(gòu)買(mǎi)現(xiàn)成的PBS溶液，不僅僅保存運(yùn)輸不便而且價(jià)格昂貴其實(shí)現(xiàn)在zui方便是使用和購(gòu)買(mǎi)PBS片劑，不僅價(jià)格只有溶液的十分之一，而且運(yùn)輸保存方便，并有多種選擇

現(xiàn)有的PBS片劑

品名	貨號(hào)	包裝	價(jià)格
Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml	09-9420-10	10 tablets/ blisterpack	726
Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml	09-9420-100	100 tablets	3,629
Phosphate Buffered Saline (PBS) pH 7.2, 1000 ml	09-9499-100	100 tablets	3,629
Phosphate Buffered Saline (PBS) pH 7.4, 100 ml	09-2051-100	100 tablets	554
Phosphate Buffered Saline (PBS) pH 7.4, 200 ml	09-2052-100	100 tablets	936
Phosphate Buffered Saline (PBS) pH 7.4, 500 ml	09-8912-100	100 tablets	2,025
Phosphate Buffered Saline (PBS) pH 7.4, 500 ml	09-8912-12	12 tablets / blister pack	478
Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml	09-9400-10	10 tablets / blister pack	726
Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml	09-9400-100	100 tablets	3,629
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 100 ml	09-8903-100	100 tablets	661
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml	09-9410-10	10 tablets / blister pack	822
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml	09-9410-100	100 tablets	4,131
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml	09-8902-100	100 tablets	2,607
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml	09-8902-12	12 tablets/ blister pack	621

其他片劑產(chǎn)品

Buffer pouches and tablets	Art Nr	Package Size	Price (SEK)
Borate Buffered Saline (BBS) pH 8.2, 500 ml	09-2053-100	100 tablets	2,598
Boric acid tablets	09-0101-1000	1000 tablets	3,209
Boric acid tablets	09-0101-100	100 tablets	726
Carbonate-bicarbonate Coating buffer with Azide pH 9.6, 100 ml	09-8932-50	50 tablets	1,414
Carbonate-bicarbonate Coating buffer with Azide pH 9.6, 100 ml	09-8932-8	8 tablets / blisterpack	669
Carbonate-bicarbonate Coating buffer pH 9.6, 100 ml	09-8922-100	100 tablets	1,108
Carbonate-bicarbonate Coating buffer pH 9.6, 100 ml	09-8922-24	24 tablets / blisterpack	669
Carbonate-bicarbonate Coating buffer pH 9.6, 100 ml	09-8922-8	8 tablets/ blisterpack	337
IMAC phosphate buffer	09-1010-10	10 tablets/ blisterpack	620
IMAC phosphate buffer	09-1010-50	50 tablets	1,244
IMAC elution buffer	09-1012-10	10 tablets/ blisterpack	726
IMAC elution buffer	09-1012-50	50 tablets	2,070
p-Nitrophenyl phosphate (pNPP) substrate 5 mg	09-2001-100	100 tablets	1,356
p-Nitrophenyl phosphate (pNPP) substrate 5 mg	09-2001-24	24 tablets/ blisterpack	917
p-Nitrophenyl phosphate (pNPP) substrate 20 mg	09-2020-100	100 tablets	3,553
p-Nitrophenyl phosphate (pNPP) substrate 20 mg	09-2020-24	24 tablets / blisterpack	974
Phosphate Buffered Saline (PBS) without Potassium	09-9500-100	100 tablets	4,202
(0.02M phosphate) pH 7.4, 1000 ml			0
Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml	09-9420-10	10 tablets/ blisterpack	726
Phosphate Buffered Saline (PBS) tablets without K pH 7.4, 1000 ml	09-9420-100	100 tablets	3,629
Phosphate Buffered Saline (PBS) pH 7.2, 1000 ml	09-9499-100	100 tablets	3,629
Phosphate Buffered Saline (PBS) pH 7.4, 100 ml	09-2051-100	100 tablets	554
Phosphate Buffered Saline (PBS) pH 7.4, 200 ml	09-2052-100	100 tablets	936
Phosphate Buffered Saline (PBS) pH 7.4, 500 ml	09-8912-100	100 tablets	2,025
Phosphate Buffered Saline (PBS) pH 7.4, 500 ml	09-8912-12	12 tablets / blister pack	478
Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml	09-9400-10	10 tablets / blister pack	726
Phosphate Buffered Saline (PBS) pH 7.4, 1000 ml	09-9400-100	100 tablets	3,629
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 100 ml	09-8903-100	100 tablets	661
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml	09-9410-10	10 tablets / blister pack	822
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 1000 ml	09-9410-100	100 tablets	4,131
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml	09-8902-100	100 tablets	2,607
Phosphate Buffered Saline with Tween® 20 (PBS-T) pH 7.4, 500 ml	09-8902-12	12 tablets/ blister pack	621
Saline Sodium Citrate (SSC 2x) pH 7.0, 100 ml	09-9501-100	100 tablets	974
Sodium Chloride tablets 0,1 mol	09-0100-100	100 tablets	614
Sodium Chloride tablets 100 ml	09-9103-100	100 tablets	535
Sodium Chloride tablets 1000 ml	09-9104-10	10 tablets/ blisterpack	458
Sodium Chloride tablets 1000 ml	09-9104-100	100 tablets	1,165
Sodium Chloride tablets 200 ml	09-9105-100	100 tablets	764
Sodium dodecyl sulphate (SDS) 0.5 g	09-2026-1000	1000 tablets	4,656
Sodium dodecyl sulphate (SDS) 0.5 g	09-2026-50	50 tablets	453
SSPE buffer 200 ml	09-8952-100	100 tablets	1,012
Tris Buffered Saline (TBS) pH 7.6, 500 ml	09-7500-100	100 tablets	2,680
Tris Buffered Saline (TBS) pH 7.6, 500 ml	09-7500-10	10 tablets / blister pack	531
Tris Buffered Saline with Tween® 20 (TBS-T) pH 7.6, 500 ml	09-7510-100	100 tablets	3,327
Tris Buffered Saline with Tween® 20 (TBS-T) pH 7.6, 500 ml	09-7510-10	10 tablets / blister pack	666
HEPES Buffered Saline (HBS) pH 7,4	09-8500-100	100 tablets	2,663
HEPES Buffered Saline (HBS) pH 7,4	09-8500-12	12 tablets / blister pack	599
Buffered Sodium Citrate 3.2 % (0.109M) 100 ml	12-8480-10	10 Pouches	860
Buffered Sodium Citrate 3.2 % powder (0.109M) 1000 ml	12-8483-5	5 Pouches	1,356
D(+)Glucose 20 % 1000 ml	12-9165-5	5 pouches	1,643
EDTA buffer pH 8.0, 1000 ml	12-9160-5	5 pouches	3,610
EDTA buffer pH 8.0, 500 ml	12-9161-5	5 pouches	2,541
Glycine-HCl buffer 0.1 M pH 3.0 1000 ml	12-9121-10	10 Pouches	953
Magnesium chloride 1 M, 1000 ml	12-9180-5	5 pouches	1,662
Magnesium sulphate 1 M, 1000 ml	12-9182-5	5 pouches	1,776
Phosphate Buffered Saline (PBS) 10x pH 7.4, 1000 ml	12-9423-5	5 pouches	955
Phosphate Buffered Saline powder (PBS) pH 7.4, 10 l	12-9424-1	1 pouch	363
Phosphate Buffered Saline powder (PBS) pH 7.4, 100 l	12-9426-1	1 pouch	2,006
Phosphate Buffered Saline powder (PBS) pH 7.4, 25 l	12-9422-1	1 pouch	783
Phosphate Buffered Saline powder (PBS) pH 7.4, 50 l	12-9425-1	1 pouch	1,223
Potassium acetate 3 M, 1000 ml	12-9170-5	5 pouches	2,923
Potassium acetate 5 M, 1000 ml	12-9171-5	5 pouches	4,050
Potassium chloride 1 M, 1000 ml	12-9175-10	10 pouches	1,681
Potassium chloride 3 M, 1000 ml	12-9176-5	5 pouches	1,452
Saline sodium citrate buffer (SSC) 20x pH 7.0, 1000 ml	12-9195-5	5 pouches	1,528
Saline sodium citrate buffer (SSC) 2x pH 7.0, 1000 ml	12-9196-5	5 pouches	764
Sodium acetate buffer pH 5.2, 1000 ml	12-9187-5	5 pouches	2,865
Sodium acetate buffer pH 7.0, 1000 ml	12-9188-5	5 pouches	2,865
Sodium chloride 3 M, 1000 ml	12-9190-5	5 pouches	764
Sodium chloride 5 M, 1000 ml	12-9191-5	5 pouches	898
Sodium dodecyl sulphate (SDS) 10 %, 1000 ml	12-9193-5	5 pouches	6,233
Sodium dodecyl sulphate (SDS) 20 %, 1000 ml	12-9194-5	5 pouches	10,739
Sodium hydroxide powder 5 M, 1000 ml	12-9183-5	5 pouches	1,318
Sodium Phosphate Buffer (NaPi) 0.02M pH 7.0, 1000 ml	12-9527-10	10 Pouches	860
Sodium Phosphate Buffer (NaPi) 0.02M pH 7.0, 5000 ml	12-9528-10	10 Pouches	2,579
Sodium phosphate buffer (NaPi) 0.1M pH 6.5, 1000 ml	12-9529-10	10 Pouches	1,089
Sodium phosphate buffer (NaPi) 0.1M pH 7.0, 1000 ml	12-9530-10	10 Pouches	1,089
Sodium phosphate buffer (NaPi) 0.1M pH 7.4, 1000 ml	12-9531-10	10 Pouches	1,089
Sodium Phosphate Buffer (NaPi) 1M pH 6.5, 1000 ml	12-9184-10	10 pouches	2,426
Sodium Phosphate Buffer (NaPi) 1M pH 7.2, 1000 ml	12-9185-10	10 pouches	2,426
Tris buffer pH 7.4, 1000 ml	12-9198-10	10 pouches	3,916
Tris buffer pH 8.0, 1000 ml	12-9199-10	10 pouches	3,916
Tris buffer pH 8.3, 1000 ml	12-9200-10	10 pouches	3,916
Tris Buffered Saline (TBS) 10x pH 8.0, 1000 ml	12-9134-10	10 pouches	6,769
Tris Buffered Saline (TBS) pH 8.0, 1000 ml	12-9133-10	10 pouches	940
Tris-Acetate-EDTA buffer (TAE) 50x pH 8.3, 1000 ml	12-9144-5	5 pouches	7,507
Tris-Acetate-EDTA buffer (TAE) 50x pH 8.3, 500 ml	12-9145-5	5 pouches	4,202
Tris-Borate-EDTA buffer (TBE) 10x pH 8.3, 1000 ml	12-9112-10	10 pouches	2,827
Tris-Borate-EDTA buffer (TBE) 5x pH 8.3, 1000 ml	12-9111-10	10 pouches	1,681
Tris-Borate-EDTA buffer (TBE) pH 8.3, 1000 ml	12-9110-10	10 pouches	726
Tris-EDTA buffer (TE) 10x pH 7.4, 1000 ml	12-9154-10	10 pouches	2,158
Tris-Glycine buffer pH 8.3, 1000 ml	12-9122-10	10 pouches	774
Tris-Glycine buffer pH 8.3, 5000 ml	12-9123-10	10 pouches	1,687
Tris-Glycine SDS buffer pH 8.3, 1000 ml	12-9222-10	10 pouches	854
Tris-Glycine SDS buffer pH 8.3, 5000 ml	12-9223-10	10 pouches	1,866
Urea 5M, 100ml	12-8481-5	5 Pouches	1,223
Urea 8M, 100 ml	12-8484-5	5 Pouches	1,509
Washing solution for DELFIA pH 7.8, 10 litres	12-9197-1	1 pouch	439

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