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        1. 上海易佰聚經(jīng)貿(mào)有限公司
          中級(jí)會(huì)員 | 第15年

          13636495876

          當(dāng)前位置:上海易佰聚經(jīng)貿(mào)有限公司>>以色列Prospec細(xì)胞因子>> 重組人GDP-5 (GDF-5)

          重組人GDP-5 (GDF-5)

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          CYT-442重組人GDP-5 (GDF-5) 2μg/10μg/1mg Prospec

           

           

          Background :
           
           
          Description :
           Recombinant Human GDF-5 produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8 Dalton.
          To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys.
          GDF5 is purified by proprietary chromatographic techniques.
           
          Physical Appearance:
           Sterile Filtered White lyophilized (freeze-dried) powder.
           
          Formulation:
           The protein was lyophilized without any additives.
           
          Solubility:
           It is recommended to reconstitute the lyophilized Recombinant GDF-5 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
           
          Stability:
           Lyophilized GDF-5 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuGDF-5 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
          Please avoid freeze-thaw cycles.
           
          Purity:
           Greater than 95.0% as determined by:
          (a) Analysis by RP-HPLC.
          (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
           
          Amino acid sequence:
           The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly.
           
          Dimers and aggregates:
           ProSpec’s Human GDF-5 is fully biologically active when compared to standard.
          Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.
           
          Endotoxin:
           Less than 0.1 ng/µg (IEU/µg) of GDF-5.
           
          Protein content:
           Protein quantitation was carried out by two independent methods:
          1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
          2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
           
          Usage:
           Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


           佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!
           

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