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        1. 上海易佰聚經(jīng)貿(mào)有限公司
          中級會員 | 第15年

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          當前位置:上海易佰聚經(jīng)貿(mào)有限公司>>以色列Prospec細胞因子>> 重組人骨形態(tài)發(fā)生蛋白(rHuBMP-4)

          重組人骨形態(tài)發(fā)生蛋白(rHuBMP-4)

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          CYT-361重組人骨形態(tài)發(fā)生蛋白(rHuBMP-4) 2μg/10μg/1mg Prospec

           

           

          Background :
           

           
          Description :
           Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton.
          BMP-4 is purified by proprietary chromatographic techniques.

           
          Physical Appearance:
           Sterile Filtered White lyophilized (freeze-dried) powder.

           
          Formulation:
           BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

           
          Solubility:
           It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

           
          Stability:
           
           Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
          Please avoid freeze-thaw cycles.

           
          Purity:
           Greater than 95.0% as determined by:
          (a) Analysis by RP-HPLC.
          (b) Anion-exchange FPLC.
          (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

           
          Amino acid sequence:
           The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His.

           
          Dimers and aggregates:
           Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

           
          Applications:
           
          1.Molecular standard (Western, ELISA) in studying secreted BMP-4
          2.Preparing antibodies for BMP-4 monomer.
          3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.

           
          Endotoxin:
           Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-4 .

           
          Protein content:
           Protein quantitation was carried out by two independent methods:

          1. UV spectroscopy at 280 nm
          2. Analysis by RP-HPLC, using a standard solution of BMP-4 as a Reference Standard.

           
          Usage:
           Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

          佰易聚生物商城提供美國光譜醫(yī)學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!

           

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