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CYT-361 | 重組人骨形態(tài)發(fā)生蛋白(rHuBMP-4) | 2μg/10μg/1mg | Prospec |
Background : | |
Description : | |
Recombinant Human BMP-4 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton. BMP-4 is purified by proprietary chromatographic techniques. | |
Physical Appearance: | |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
Formulation: | |
BMP-4 was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. | |
Solubility: | |
It is recommended to reconstitute the lyophilized BMP-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized BMP-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
Purity: | |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Amino acid sequence: | |
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Lys-His-His. | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Applications: | |
1.Molecular standard (Western, ELISA) in studying secreted BMP-4 2.Preparing antibodies for BMP-4 monomer. 3.Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of Bone Morphogenetic protein-4 . | |
Protein content: | |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm 2. Analysis by RP-HPLC, using a standard solution of BMP-4 as a Reference Standard. | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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