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CYT-263

重組內(nèi)皮生長因子-E（Orf 病毒）（rov-VEGF-E）

2μg/10μg/1mg

Prospec

Description :
	A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 （Dehio et al., 1999; GenBank accession No. AF106020） was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximay 35 kDa. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus （OV）（Lyttle et al., 1994）. Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins （Dehio et al., 1999; Wise et al., 1999）. Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 （KDR） resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 （Flt-1）. Furthermore VEGF-E can also not bind to VEGF receptor-3 （FLT-4）. Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The protein was lyophilized from a concentrated （1mg/ml） solution containing 0.5x PBS pH-7.4.
	Solubility:
	It is recommended to reconstitute the lyophilized ov-VEGF-E in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized ov-VEGF-E although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution ov-VEGF-E should be stored at 4⁰C. between 2-7 days and for future use below -18⁰C. Please prevent freeze-thaw cycles.
	Purity:
	Greater than 90.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for ov-VEGF-E has been determined to be in the range of 5 - 20 ng/ml, corresponding to a specific activity of 5 x 10³ Units/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of ov-VEGF-E.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of ov-VEGF-E as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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