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        1. 上海易佰聚經(jīng)貿(mào)有限公司
          中級(jí)會(huì)員 | 第15年

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          當(dāng)前位置:上海易佰聚經(jīng)貿(mào)有限公司>>以色列Prospec細(xì)胞因子>> 重組人血管內(nèi)皮生長(zhǎng)因子-121(rHuVEGF121)

          重組人血管內(nèi)皮生長(zhǎng)因子-121(rHuVEGF121)

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          CYT-343重組人血管內(nèi)皮生長(zhǎng)因子-121(rHuVEGF121) 2μg/10μg/1mg Prospec

           

           

          Background:
           Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent.
          In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.

           
          Description :
           Recombinant Human VEGF-121 produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton. VEGF-121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.

          Recombinant VEGF is purified by proprietary techniques.

           
          Physical Appearance:
           Sterile Filtered White lyophilized (freeze-dried) powder.

           
          Formulation:
           Recombinant VEGF was lyophilized from a concentrated (1mg/ml) solution with no additives.

           
          Solubility:
           It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

           
          Stability:
           
           Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuVEGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

          Please prevent freeze-thaw cycles.

           
          Purity:
           Greater than 98.0% as determined by:
          (a) Analysis by RP-HPLC.
          (b) Anion-exchange FPLC.
          (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

           
          Amino acid sequence:
           The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.

           
          Dimers and aggregates:
           Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

           
          Biological Activity:
           The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml.

           
          Endotoxin:
           Less than 0.1 ng/µg (IEU/µg) of VEGF.

           
          Protein content:
           Protein quantitation was carried out by two independent methods:

          1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
          2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard.

           
          Usage:
           Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


           佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!
           

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