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當(dāng)前位置:上海易佰聚經(jīng)貿(mào)有限公司>>以色列Prospec細(xì)胞因子>> 重組人血管內(nèi)皮生長(zhǎng)因子-121(rHuVEGF121)
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更新時(shí)間:2024-05-23 10:15:19瀏覽次數(shù):796次
聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)重組人血管內(nèi)皮生長(zhǎng)因子VEGF-CHO
重組人血管內(nèi)皮生長(zhǎng)因子VEGF-His Tag
重組人血小板生成素TPO/Thrombopoietin CHO
CYT-343 | 重組人血管內(nèi)皮生長(zhǎng)因子-121(rHuVEGF121) | 2μg/10μg/1mg | Prospec |
Background: | |
Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating Vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. | |
Description : | |
Recombinant Human VEGF-121 produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of 28423 Dalton. VEGF-121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. Recombinant VEGF is purified by proprietary techniques. | |
Physical Appearance: | |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
Formulation: | |
Recombinant VEGF was lyophilized from a concentrated (1mg/ml) solution with no additives. | |
Solubility: | |
It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuVEGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | |
Purity: | |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Amino acid sequence: | |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu. | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Biological Activity: | |
The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.2-0.4 ng/ml. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of VEGF. | |
Protein content: | |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of VEGF as a Reference Standard. | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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