在2017年12月，由Xulei Qin，Huaxiao Yang，Haodi Wu，Xin Zhao等作者在Advanced Functional Materials中發(fā)表一篇了《Photoacoustic Imaging of Embryonic Stem Cell-Derived Cardiomyocytes in Living Hearts with Ultrasensitive Semiconducting Polymer Nanoparticles》的文章。

文章使用Revolve正倒置一體顯微鏡獲得了如下結(jié)果：

該圖中B是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

該圖中E、F是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

該圖是通過Revolve正倒置一體顯微鏡成像得到的結(jié)果 ↑

中文摘要：

人類胚胎干細(xì)胞起源的心肌細(xì)胞有望成為修復(fù)心臟損傷強(qiáng)有力的工具。為了獲得好的結(jié)果，我們需要追蹤心臟移植細(xì)胞，因此*的分子成像技術(shù)是至關(guān)重要的。本研究中發(fā)現(xiàn)，將一類含有半導(dǎo)體聚合物（SPs）的光聲納米粒子（PANPS）作為造影劑，可用于活體小鼠心臟移植hESC-CMs的光聲成像（PAI）。這得益于PANPs的兩個(gè)優(yōu)勢。首先，強(qiáng)光聲信號(hào)（PA）和SPs特定光譜曲線使得PAI從背景組織中檢測和區(qū)分少量PANP標(biāo)記的細(xì)胞（2000）具有很高的靈敏度。其次，PANP對(duì)hESC-CM的標(biāo)記效率高，并且也不影響細(xì)胞的結(jié)構(gòu)、功能和基因表達(dá)。通過超聲成像，我們可以利用PANP依賴的高空間分辨率（≈100um）的PAI來評(píng)估hESC-CM在活體小鼠心臟中的移植狀態(tài)。綜上所述，本研究探索并驗(yàn)證了SPs新的應(yīng)用方向，可作為PA造影劑實(shí)現(xiàn)對(duì)體內(nèi)標(biāo)記細(xì)胞高靈敏度、高準(zhǔn)確度的示蹤，凸顯了PAI與PANPs結(jié)合在心臟再生治療上的優(yōu)勢。

英文摘要：

Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) have become promising tools to repair injured hearts. To achieve optimal outcomes,advanced molecular imaging methods are essential to accurately track these transplanted cells in the heart. In this study, it is demonstrated for the first time that a class of photoacoustic nanoparticles (PANPs) incorporating semiconducting polymers (SPs) as contrast agents can be used in the photoacoustic imaging (PAI) of transplanted hESC-CMs in living mouse hearts. This is achieved by virtue of two benefits of PANPs. First, strong photoacoustic (PA) signals and specific spectral features of SPs allow PAI to sensitively detect and distinguish a small number of PANP-labeled cells(2000) from background tissues. Second, the PANPs show a high efficiency for hESC-CM labeling without adverse effects on cell structure, function, and gene expression. Assisted by ultrasound imaging, the delivery and engraftment of hESC-CMs in living mouse hearts can be assessed by PANP-based PAI with high spatial resolution (≈100 μm). In summary, this study explores and validates a novel application of SPs as a PA contrast agent to track labeled cells with high sensitivity and accuracy in vivo, highlighting the advantages of integrating PAI and PANPs to advance cardiac regenerative therapies.

ECHO Revolve正倒置一體顯微鏡展現(xiàn)了其非凡的靈活性，可以輕松地實(shí)現(xiàn)正置和倒置顯微鏡轉(zhuǎn)換，創(chuàng)新性地把正倒置顯微鏡合二為一，開啟了顯微鏡Hybrid時(shí)代。

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