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閱讀：668 發(fā)布時間：2014-2-11文獻名：Lung exposure to nanoparticles modulates an asthmatic response in a mouse model
作者：S. Hussain*,#,e, J.A.J. Vanoirbeek#,e, K. Luyts#, V. De Vooght#, E. Verbeken", L.C.J. Thomassen+, J.A. Martens+, D. Dinsdale1, S. Boland*, F. Marano*, B. Nemery# and P.H.M. Hoet#
*Laboratory of Molecular and Cellular Responses to Xenobiotics, Unit of Functional and Adaptive Biology, University of Paris Diderot, Paris, France.
#Research Unit for Lung Toxicology.
"Morphology and Molecular Pathology Section, and
+Center for Surface Chemistry & Catalysis, KU Leuven, Leuven, Belgium.
1MRC Toxicology Unit, University of Leicester, Leicester, UK.
eThese authors contributed equally to the study.
摘要：The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO2) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanateinduced asthma.
On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone–olive oil (AOO) on the dorsum of both ears (20 mL). On day 14, the mice were oropharyngeally dosed with 40 mL of a NP suspension (0.4 mg?mL-1 (,0.8 mg?kg-1) TiO2 or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 mL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed.
NP exposure in sensitised mice led to a two- (TiO2) or three-fold (Au) increase in AHR, and a three- (TiO2) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation.
In conclusion, these results show that a low, intrapulmonary doses of TiO2 or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.
關(guān)鍵詞：Diisocyanate-induced asthma, gold, nanoparticle, titanium dioxide, toluene diisocyanate