供貨周期 |
現(xiàn)貨 |
規(guī)格 |
1 kit |
貨號 |
AS-72129 |
產(chǎn)品簡介:
產(chǎn)品品牌:AnaSpec
產(chǎn)品名稱:SensoLyte®520凝血mei活性測定試劑盒熒光法-1試劑盒
英文名稱:SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit
產(chǎn)品貨號:AS-72129
產(chǎn)品規(guī)格:1 kit
AnaSpec 凝血mei活性測定試劑盒熒光法
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性測定試劑盒熒光法-1試劑盒
我們北京云肽生物科技有限公司作為AnaSpec公司的經(jīng)銷商為客戶提供,正規(guī)進(jìn)口,保證原裝正品,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。
AnaSpec ( 美國 )
AnaSpec公司成立于1993年,是一家為全球生命科學(xué)研究,提供綜合蛋白質(zhì)組學(xué)和基因組學(xué)解決方案的領(lǐng)xian供應(yīng)商。
公司產(chǎn)品主要分三大部分:提供專業(yè)多肽,檢測試劑,抗體檢測試劑盒;適合基礎(chǔ)研究的高通量篩選和新藥物研發(fā)相關(guān)試劑;多肽合成,抗體生產(chǎn),寡核苷酸的合成等定制服務(wù)。
AnaSpec 凝血mei活性測定試劑盒熒光法
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mie活性測定試劑盒熒光法-1試劑盒,產(chǎn)品簡介:
產(chǎn)品品牌:AnaSpec
產(chǎn)品名稱:SensoLyte®520凝血mei活性測定試劑盒熒光法-1試劑盒
英文名稱:SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit
產(chǎn)品貨號:AS-72129
產(chǎn)品規(guī)格:1 kit
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性測定試劑盒熒光法-1試劑盒,產(chǎn)品說明:
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion. The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.
凝血mei是一種絲氨suan蛋白酶,通過將可溶性血漿纖維蛋白原轉(zhuǎn)化為不溶性纖維蛋白凝塊和促進(jìn)血小板聚集,在止血中發(fā)揮中心作用。凝血mei還影響許多正常和病理過程,包括炎癥、組織修復(fù)、胚胎發(fā)生、血管生成和腫瘤侵襲。SensoLyte®520凝血mei分析試劑盒經(jīng)過優(yōu)化,可用于檢測凝血mei活性。該套件包含一種新型內(nèi)部淬火5-FAM/QXL™ 520凝血mei的FRET底物。凝血mei將FRET底物切割成兩個(gè)單獨(dú)的片段,導(dǎo)致5-FAM熒光的釋放,可在激發(fā)/發(fā)射=490 nm/520 nm時(shí)進(jìn)行監(jiān)測。5-FAM的長波熒光受生物樣品和供試化合物中成分的自發(fā)熒光干擾較小。該分析可在96孔或384孔微孔板中進(jìn)行,以篩選酶抑制劑。
Component A: 5-FAM/QXL™520 Thrombin substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: 5-FAM, Fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: 2X Assay buffe:r 20 mL Component D: Purified human thrombin enzyme: 0.1 mg/mL, 40 µL Component E: Thrombin inhibitor: 60 µM, 10 µL Component F: Stop solution: 5 mL
組件A:5-FAM/QXL™520凝血mei底物,Ex/Em=490 nm/520 nm切割后:2 mM,50µL組分B:5-FAM,熒光參考標(biāo)準(zhǔn),Ex/Em=490 nm/520 nm:2 mM,10µL組分C:2X分析緩沖液:r 20 mL組分D:純化人凝血mei:0.1 mg/mL,40µL組分E:凝血mei抑制劑:60µM,10µL組分F:停止液:5 mL
AnaSpec 凝血mei活性測定試劑盒熒光法
產(chǎn)品訂購信息
AnaSpec AS-72129 SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit,SensoLyte®520凝血mei活性測定試劑盒熒光法-1試劑盒 1 kit