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當(dāng)前位置：BPS Bioscience Inc.>>Ubiquitination>> 78542DCAF11 Intrachain TR-FRET Assay Kit
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產(chǎn)品型號(hào)78542
品 牌BPS Bioscience
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1,10-Phenanthroline, Monohydrate
A20, His tag, FLAG-tag (Sf9-derived
The DCAF11 Intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit designed to measure DCAF11 auto-ubiquitination activity in a homogeneous 384-reaction format. It utilizes Europium-labeled Ubiquitin (donor) and Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. This assay measures poly-ubiquitination since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on DCAF11. As a homogenous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.
Figure 1. DCAF11 Intrachain TR-FRET Assay Kit Schematic.
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