The Keap1:Nrf2 Inhibitor Screening Assay Kit is designed for identification of inhibitors of Keap1:Nrf2 binding using fluorescence polarization (FP), a powerful tool for studying molecular interactions by monitoring the change in rotational mobility of the fluorescently-labeled molecules upon their binding to a partner. To determine the effect of the inhibitor on the formation of Keap1:Nrf2 complexes the Keap1 protein and the fluorescent Nrf2 peptide are incubated with or without the test inhibitor for 30 minutes. Changes in rotational mobility of the Nrf2 peptide are measured using a plate reader capable of measuring fluorescence polarization.

Figure 1. Illustration of the Keap1:Nrf2 Inhibitor Screening Assay Kit.

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