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當(dāng)前位置：BPS Bioscience Inc.>>Ubiquitination>> 78428NEDD4 intrachain TR-FRET Assay Kit
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產(chǎn)品型號(hào)78428
品 牌BPS Bioscience
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更新時(shí)間：2023-11-12 22:11:01瀏覽次數(shù)：117次
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1,10-Phenanthroline, Monohydrate
A20, His tag, FLAG-tag (Sf9-derived
The NEDD4 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure NEDD4 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on NEDD4, this assay measures poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.
Figure 1. E3 ligase NEDD4 intrachain TR-FRET Assay Kit schematic.
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