The forkhead box P3 (Foxp3, also known as scurfin) Luciferase Reporter Jurkat cell line is designed for monitoring the transcriptional activity of the Foxp3 promoter and testing inhibitors that target Foxp3-inducing signaling pathways. It contains a firefly luciferase gene under the control of a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.

This reporter cell line has been tested and validated using phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin. It can be used to measure T cell activation mediated by Dynabeads T-Activator CD3/CD28.

Figure 1: Illustration of the Foxp3 promoter region with representative transcription factor binding sites and enhancer regions, and illustration of the cell-based assay principle (created with BioRender.com).

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