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        1. BPS Bioscience Inc.
          中級會員 | 第2年

          當前位置:BPS Bioscience Inc.>>試劑>>克隆與表達>> AAV8 SaCas9

          AAV8 SaCas9

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          產(chǎn)品型號

          品       牌BPS Bioscience

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          所  在  地

          更新時間:2023-10-10 23:07:54瀏覽次數(shù):134次

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          保存條件:-80°C(dryice)保質(zhì)期:3-18month英文名:AAV8SaCas9數(shù)量:1供應(yīng)商:BPSBioscienceInc
          • 保存條件:

            -80°C (dry ice)

          • 保質(zhì)期:

            3-18 month

          • 英文名:

            AAV8 SaCas9

          • 數(shù)量:

            1

          • 供應(yīng)商:

            BPS Bioscience Inc.

          • CAS號:

            /

          • 規(guī)格:

            50 µl x 2

          Adeno-Associated Virus serotype 8 (AAV8) was isolated from rhesus monkey tissue, and the AAV8 rep and cap nucleotide sequences have 88% homology with AAV7 and 82% with AAV2. AAV8 exhibits greater transduction efficiency in the liver than other AAV serotypes. AAV8 and 9 have recently been used to correct disease-causing mutations and improve muscle function in mouse models of Duchenne muscular dystrophy.Cas9 is an endonuclease enzyme that introduces a double stranded break into the DNA when recruited to a specific DNA sequence by the sgRNA (single guide RNA). This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the gene.SaCas9 (Staphylococcus aureus CRISPR-associated protein 9) has demonstrated high cutting efficiency in mammalian cells, and its smaller size makes it ideal for packaging into AAV. SaCas9 recognizes a longer protospacer adjacent motif (PAM) site, 5'-NNGRRT-3', than the more traditional SpCas9 (Streptococcus pyogenes CRISPR-associated protein 9). These AAV particles constitutively express SaCas9 under the control of a CMV promoter.
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