細(xì)胞培養(yǎng)器材大全
細(xì)胞培養(yǎng)皿:
產(chǎn)品編號(hào) | 產(chǎn)品描述 | 建議上液量 |
430165 | 規(guī)格:35mm;高度:10mm;生長面積:8cm2 | 3ml |
430166 | 規(guī)格:60mm;高度:15mm;生長面積:21cm2 | 5ml |
430196 | 規(guī)格:60mm;高度:15mm;生長面積:21cm2 | 5ml |
430167 | 規(guī)格:100mm;高度:20mm;生長面積:55cm2 | 10ml |
430293 | 規(guī)格:100mm;高度:20mm;生長面積:55cm2 | 10ml |
430599 | 規(guī)格:150mm;高度:25mm;生長面積:148cm2 | 30ml |
431110 | 規(guī)格:245mm(方形);高度:25mm;生長面積:500cm2 | 100ml |
431112 | 規(guī)格:245mm(方形);高度:25mm;生長面積:500cm2 | 100ml |
細(xì)胞培養(yǎng)瓶:
產(chǎn)品編號(hào) | 產(chǎn)品描述 | 建議上液量 |
430639 | 規(guī)格:25cm2,矩形,斜頸,透氣蓋 | 5ml |
430641 | 規(guī)格:75mm2,矩形,斜頸,透氣蓋 | 15ml |
430825 | 規(guī)格:150cm2,矩形,斜頸,透氣蓋 | 30ml |
431080 | 規(guī)格:175cm2,矩形,角度頸,透氣蓋 | 35ml |
3001 | 規(guī)格:225cm2,矩形,斜頸,透氣蓋 | 45ml |
產(chǎn)品編號(hào) | 產(chǎn)品描述(單孔面積,體積)(128 x 86) | 建議上液量 |
| 規(guī)格:6孔板,9.5 cm2,16.5mL | 2.5-3.0ml |
| 規(guī)格:12孔板,3.9cm2,6.5mL | 2.0ml |
| 規(guī)格:24孔板,1.75cm2,2.9ml | 1.0ml |
| 規(guī)格:48孔板,1.0cm2,1.7ml | 0.5ml |
| 規(guī)格: 96孔板,0.32cm2,0.37mL(平底) 96孔板,0.35cm2,0.30mL(U-底) 96孔板, 0.28cm2,0.23ml(V-底) | 0.1-0.25ml |
| 規(guī)格:384孔板,0.1 cm2, 0.12ml | 0.06ml |
| 規(guī)格:1536孔板,0.02 cm2,0.012ml | 0.015ml |
| 規(guī)格:4孔板,16.0cm2(132 x 82) | 5.0ml |
| 規(guī)格:8孔板,8.6cm2(132 x 82) | 3.0ml |
| 規(guī)格:4孔板,21.8cm2(128 x 86) | 5.0ml |
細(xì)胞培養(yǎng)板的選擇
培養(yǎng)器皿 | 底面積(cm2) | 加培養(yǎng)液量(mL) | 可獲細(xì)胞量 |
96孔培養(yǎng)板 | 0.32 | 0.1 | 105 |
24孔培養(yǎng)板 | 2 | 1.0 | 5×105 |
12孔培養(yǎng)板 | 4.5 | 2.0 | 106 |
6孔培養(yǎng)板 | 9.6 | 2.5 | 2.5×106 |
4孔培養(yǎng)板 | 28 | 5.0 | 7×106 |
3.5cm培養(yǎng)皿 | 8 | 3.0 | 2.0×106 |
6cm培養(yǎng)皿 | 21 | 5.0 | 5.2×106 |
9cm培養(yǎng)皿 | 49 | 10.0 | 12.2×106 |
10cm培養(yǎng)皿 | 55 | 10.0 | 13.7×106 |
25cm塑料培養(yǎng)瓶 | 25 | 5.0 | 5×106 |
75cm塑料培養(yǎng)瓶 | 75 | 15~30 | 2×107 |
25cm玻璃培養(yǎng)瓶 | 19 | 4.0 | 3×106 |
100cm玻璃培養(yǎng)瓶 | 37.5 | 10.0 | 6×106 |
250cm玻璃培養(yǎng)瓶 | 78 | 15.0 | 2×107 |
2500cm旋轉(zhuǎn)培養(yǎng)瓶 | 700 | 100~250 | 2.5×108 |
24孔板:PLL 100-200μl(200μl)即可,鋪板2h后,吸出PLL(回收),PBS沖洗2次,晾干備用或不晾干直接用。加培養(yǎng)液量1ml ,注意調(diào)整細(xì)胞密度。
25cm2(5ml)→(1:2)50cm2(10ml)——(1:3)75cm2(15ml)
2cm2(1ml)→1/25——1/40總細(xì)胞懸液
96孔板:PLL 20-40μl(40μl)即可,鋪板2h后,吸出PLL(回收),PBS沖洗2次,晾干備用或不晾干直接用。加培養(yǎng)液量0.1ml-0.2ml ,注意調(diào)整細(xì)胞密度。
25cm2(5ml)→(1:2)50cm2(10ml)——(1:3)75cm2(15ml)
0.32cm2(0.1-0.2ml)→1/150——1/250總細(xì)胞懸液