詳細(xì)介紹
青色素CY5.5酰肼染料 Cy5.5 hydrazide
Cy5.5 hydrazide是含羰基的近紅外活性染料。酰肼可以與一些羰基化合物迅速反應(yīng),例如醛類和酮類,并能形成穩(wěn)定的腙。該反應(yīng)可用于標(biāo)記偶聯(lián)物的制備。生物分子中的羰基基團(tuán)可以在氧化應(yīng)激或蛋白質(zhì)的脫氨基作用之后自發(fā)的形成。或者,醛基可以通過(guò)糖蛋白分子糖殘基的高碘酸氧化特異生成,例如抗體??贵w可以被高碘酸鈉氧化,并且可以被Cy5.5酰肼標(biāo)記,從而形成Cy5.5酰肼標(biāo)記的抗體。由于抗體中的糖殘基遠(yuǎn)離可變的表位結(jié)合位點(diǎn),用這種方法標(biāo)記抗體是很理想的。
青色素CY5.5酰肼染料 Cy5.5 hydrazide
General properties:
Appearance: dark blue powder
Molecular weight: 669.73
Molecular formula: C40H46Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties:
Excitation maximum, nm: 684
Emission maximum, nm:710
Fluorescence quantum yield:0.2
CF260:0.07
CF280:0.03
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