目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs00839Recombinant Human GM-CSF（5ug）
| 供貨周期 | 一周 | 規(guī)格 | 5ug |
|---|---|---|---|
| 貨號 | abs00839 |
| 概述 | |
|---|---|
| 種屬 | Human |
| 形態(tài) | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| 來源 | Escherichia coli. |
| 氨基酸序列 | APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE |
| 內(nèi)毒素水平 | Less than 1.0 EU/μg of Recombinant HumanGM-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Accession # | P04141 |
| 預(yù)期分子量 | Approximay 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 純度 | > 98 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 1437 |
| 參考文獻(xiàn) | |
|---|---|
| 1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.<br/>2. 1989. N Engl J Med, 320: 253-4.<br/>3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.<br/>4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.<br/>5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31. | |
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