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Monolith Reversed-Phase Columns for High-Resolution Protein Separations at High Flow Rates
ProSwift® reversed-phase monolith columns uniquely provide the advantages of high resolution at exceptionally high flow rates for protein separations. They deliver the outstanding resolving power of nonporous analytical media combined with faster separations than any bead based columns available. ProSwift polymer monolith columns achieve long column lifetime, exhibit reproducibility even after hundreds of runs, and have broad pH stability, providing high stringent wash capability.
High resolution at high speed
Highest operational flow rates available
High throughput and improved productivity
Optimal performance in a broad range of applications
Excellent stability over a wide pH range of 1 to 14
Outstanding reproducibility and ruggedness
High stringent wash compatible, for example, 1 M NaOH
High loading capacity
ProSwift polymer monolith media are uniquely suited for the separation of proteins. Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith’s nonporous surfaces result in fast mass transfer for high-resolution and fast protein separations. The channels also produce low backpressure, allowing the use of higher linear velocities with minimal loss of resolution.
| ProSwift Reversed-Phase Monolith Columns Specifications | ||||||
|---|---|---|---|---|---|---|
| Column Name | Proswift RP-15 | Proswift RP-2H | ProSwift RP-3U | Proswift RP-4H | ProSwift RP-10R | |
| Column Dimensions | 4.6 × 50 mm | 4.6 × 50 mm | 4.6 × 50 mm | 1 × 50 mm | 1 × 250 mm | 1 × 50 mm |
| Base Matrix Material | Polystyrene-divinlbenzene | |||||
| Surface Chemistry | Phenyl | |||||
| Protein Binding Capacity per mL of Polymer | 3.0 mg/mL Lysozyme | 1.0 mg/ml Lysozyme | .05 mg/ml Lysozyme | 2.3 mg/ml Lysozyme | n.d.* | |
| Protein binding capacity per column | 2.2 mg Lysozyme | 0.8 mg Lysozyme | 0.3 mg Lysozyme | 0.1 mg Lysozyme | 0.5 mg Lysozyme | n.d. |
| Bed Height | 46 mm | 44 mm | 40 mm | 50 mm | 250 mm | 50 mm |
| Bed Volume (mL) | 0.76 mL | 0.73 mL | 0.65 mL | 0.039 mL | 0.196 mL | 0.039 mL |
| pH Range | 1–14 | 1–10 | ||||
| Recommended Flow Rate | 0.5–4 mL/min | 1–10 mL/min | 1–16 mL/min | 0.1–0.4 mL/min | 0.1–0.3 mL/min | 40–60 µL/min |
| Maximum Flow Rate | 5 mL/min | 14 mL/min | 20 mL/min | 0.5 mL/min | 0.4 mL/min | 100 µL/min |
| Maximum Pressure | 2800 psi | <2000 psi 13.8 Mpa | 3000 psi 20.7 MPa | 4350 psi 30 MPa | ||
| Operating Pressure | < 1500 psi | < 2500 psi 17.5 MPa | 2900 psi 20 MPa | |||
| Temperature | 70 °C | 80 °C | ||||
| Solvent Compatability | Most Common organic solvents | |||||
*n.d.=not determined