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          上海宸喬生物科技有限公司>>Ultrasep ES液相色譜柱>>Clinical Chemistry(臨床化學(xué))>>Amino Acids and Peptides

          Amino Acids and Peptides

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          • Amino Acids and Peptides

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          The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

          詳細(xì)介紹

          Amino Acid and Peptide Analysis

           

          UltraSep ESD AP

            

          The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

          Comparison of different procedures for derivatization

          Procedure

          Detection

          Limit of detection

          Remarks

          DABS

          Absorption
          436 nm

          < 1 pmol

          Stable derivatives; primary and secondary amino acids can be determined

          OPA

          Fluorescence
          330/440 nm

          < 1 pmol

          Pro, Hypro and Cys cannot be determined 

          FMOC

          Fluorescence
          265/315 nm

          < 1 pmol

          His and Cys will be detected only in part

          DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
          OPA = o-Phthalaldehyde
          FMOC = 9-Fluorenylmethoxycarbonylchloride

           

          Examples of Analyzed Compounds

          Analyzed in Application

          Go to

           

          Alanine (Ala)

          AA

           

           

          Arginine (Arg)

          AA

          AA

           

          Aspartic acid (Asp)

          AA

           

           

          Cystine (Cys2)

          AA

          EPHARM6

           

          Glutamic acid (Glu)

          AA

           

           

          Glycine (Gly)

          AA

           

           

          Glutathione

          EPHARM6

           
           

          Histidine (His)

          AA

           

           

          Isoleucine (Ile)

          AA

           

           

          Leucine (Leu)

          AA

           

           

          Lysine (Lys)

          AA

           

           

          Methionine (Met)

          AA

           

           

          Ornithine (Orn)

          AA

           

           

          Phenylalanine (Phe)

          AA

           

           

          Proline (Pro)

          AA

           

           

          Serine (Ser)

          AA

           

           

          Threonine (Thr)

          AA

           

           

          Tyrosine (Tyr)

          AA

           

           

          Valine (Val)

          AA

           

           

          COLUMNS and  PRICES  ()
           Column size (mm)
          250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
          UltraSep ESD AP360.00340.00355.00320.00295.00290.00
           

          Size (mm)

           
          10 x 410 x 310 x 2
          Precolumn cartridges
          (Price per pack = 5 pieces)
          170.00170.00170.00

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