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產品英文名稱：CHO-CM HCP ELISA kit
產品中文名稱：CHO-CM HCP ELISA 試劑盒
CHO-CM HCP ELISA kit 的應用
本試劑盒是用于確定在CHO細胞重組表達產品中CHO宿主細胞蛋白（HCPS）的存在。本試劑盒適合使用的抗體通常發(fā)現在無蛋白的條件培養(yǎng)介質相生成。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。
CHO-CM HCP ELISA 試劑盒的英文描述
This assay is intended for use on pharmaceutical products secreted into protein-free CHO growth media. The antibody is raised against, affinity purified, and calibrated with HCPs typically found in CHO protein-free conditioned media. Special procedures were utilized in the generation of these antibodies to insure that low molecular weight and less immunogenic contaminants as well as high molecular weight components would be represented. Our assay validation has determined that the LOD for this kit is <200pg/mL.
CHO-CM HCP ELISA kit 的使用步驟規(guī)則
The CHO HCP assay is a sequential, two-site immunoenzymetric assay also termed ELISA. Samples potentially containing CHO proteins are reacted in microtiter strips coated with an affinity purified capture antibody. After a wash step, a horseradish peroxidase enzyme labeled anti-CHO antibody is reacted sequentially forming a sandwich complex of solid phase antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of CHO proteins present.
CHO-CM HCP ELISA kit 的保存和穩(wěn)定性
* All reagents should be stored at 2C to 8C for stability until the expiration date printed.
*Reconstituted wash solution is stable until the expiration date of the kit.
CHO-CM HCP ELISA kit 的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5M H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
*本試劑盒只能由合格的技術人員使用。
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