目錄:上海華雅思創(chuàng)生物科技有限公司>>細(xì)胞培養(yǎng)>>DC-CIK治療細(xì)胞治療>> Cygnus_Technologies F015 CHO 細(xì)胞（CHO ELISA kit） ELISA kit
產(chǎn)品英文名稱：CHO ELISA kit
產(chǎn)品中文名稱：CHO 細(xì)胞 ELISA 試劑盒
CHO ELISA kit 的應(yīng)用
本試劑盒是用于確定在CHO宿主細(xì)胞重組表達(dá)產(chǎn)品 中CHO蛋白污染存在。該試劑盒只用于研究和制造使用，不適合于人類或動物的診斷應(yīng)用。
：，客服
CHO細(xì)胞 ELISA 試劑盒的英文描述
The antibodies used in the kit were generated against and affinity purified from a cell lysate of CHO cells grown in serum supplemented media. All three of the Cygnus CHO HCP kits recognize a qualitatively vast majority of the same HCPs. The potential exists that the quantitative value reported for a given sample type may differ between the kits, therefore we cannot predict which kit will be most suitable for your applications. Formal qualification must be performed on each different product, to determine which of the 3 kits are superior. For labs looking at CHO HCP contamination for the first time, we suggest evaluating our CHO HCP ELISA, 3G, Catalog # F550. If this method proves inadequate then we suggest evaluating CHO HCP kits, Catalog #CM015 and F015.
CHO ELISA kit 的使用步驟規(guī)則
The CHO Host Cell Protein assay is a two-site immunoenzymetric assay. Samples which may contain CHO proteins are reacted with an affinity purified alkaline phosphatase labeled antibody. This reaction either takes place in a test tube in a sequential assay mode or simultaneously in microtiter wells coated with an anti-CHO HCP capture antibody.The two-step sequential protocol or the one step simultaneous
protocol result in the formation of a sandwich complex of solid phase (capture) antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of CHO proteins present.
CHO ELISA kit 的保存和穩(wěn)定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.
CHO ELISA kit 的使用注意事項
*只用于研究或制造。
*在用本試劑盒的試劑濃度，其他沒有被認(rèn)為是有害的。
*本試劑盒只能由合格的技術(shù)人員使用。
一切干細(xì)胞實驗耗材優(yōu)惠盡在華雅思創(chuàng)生物
2015年統(tǒng)統(tǒng)*，--一切活動盡在上海華雅思創(chuàng)！
：，客服
StemRD MGro-500 MSC medium,chemically-defined,間充質(zhì)干細(xì)胞培養(yǎng)基 500mL/瓶
LONZA 04-418Q X-VIVO 15 無血清培養(yǎng)基 1L/瓶
Life Technology 087-0112DK AIM-V® CTS™ 無血清細(xì)胞培養(yǎng)基（細(xì)胞治療級） 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium無血清培養(yǎng)基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA樣本收集管 100管/盒
LONZA 50150 SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶
LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套
Nunc 159910 175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過濾蓋 30個/箱
LONZA LT07-318 MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 100Tests/盒
LONZA LT07-418 MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 50Tests/盒
Lonza 50-650U Kinetic-QCL 192 Test Kit內(nèi)毒素檢測試劑盒 192T/盒
Corning 431080 175cm⒉細(xì)胞培養(yǎng)瓶，Angled頸，透氣蓋 50個/箱
Corning 8388 125mL儲液瓶 Storage Bottles,PS,45mm瓶頸，帶蓋，滅菌，獨立包裝 24個/箱
Corning 430659 2ml凍存管, 聚丙烯，外旋蓋, 可自立圓底 500個/箱
Corning 3290 75cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 100個/箱
Corning 3292 175cm2 CellBIND Surface培養(yǎng)瓶，已滅菌，透氣蓋 50個/箱
BD-Falcon 352360 Cell Strainer, 100µm, Yellow 100um細(xì)胞濾網(wǎng) 50個/箱
BD Falcon 352070 50mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱
BD Falcon 352096 15mL錐形離心管，高透明度聚丙烯,袋裝 500支/箱
BD BioCoat 354234 Matrigel Basement Membrane Matrix(標(biāo)準(zhǔn)型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白檢測試劑盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大腸桿菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個/箱
Nalgene 5026-0909 CryoBoxes 凍存盒 9X9 陣列，管瓶1.2/2.0mL 24個/箱
Nalgene 2250-0130 50L 可高溫高壓操作的細(xì)口大瓶（帶手柄） 1個/箱
Nalgene 2319-0050 20L 可高溫高壓滅菌的細(xì)口大瓶（帶放水口） 1個/箱
Hyclone SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm® M Laboratory Film 封口膜 12卷/箱
(空格分隔,最多3個,單個標(biāo)簽最多10個字符)
5
化工儀器網(wǎng)