目錄:上海華雅思創(chuàng)生物科技有限公司>>細胞培養(yǎng)>>DC-CIK治療細胞治療>> Cygnus_Technologies F220 NS/0 HCP ELISA 試劑盒
產(chǎn)品英文名稱:NS/0 HCP ELISA kit
產(chǎn)品中文名稱:NS/0 HCP ELISA 試劑盒
:,客服
NS/0 HCP ELISA kit 的應(yīng)用
本試劑盒是用于確定在NS/0 的重組表達產(chǎn)品細胞中NS/0 宿主細胞蛋白污染的存在。該試劑盒是用于研究和制造使用,不用于人類或動物的診斷應(yīng)用。
NS/0 HCP ELISA 試劑盒的英文描述
Hybridization of antibody producing cells with NS/0 cells is one of the most commonly employed systems for the production of humanized and mouse monoclonal antibodies. This kit has been successfully qualified for a number of different therapeutic human monoclonal antibodies. Our assay validation has determined that the LOD for this kit is ~300pg/mL.
NS/0 HCP ELISA kit 的使用步驟規(guī)則
The NS/0 assay is a two-site immunoenzymetric assay. Samples containing NS/0 HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-NS/0 antibody in a test tube or simultaneously in microtiter strips coated with an affinity purified capture anti-NS/0 antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of NS/0 HCPs present.
NS/0 HCP ELISA 試劑盒的保存和穩(wěn)定性
1 All reagents should be stored at 2℃ to 8C℃for stability until the expiration date printed.
2 The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
3 Reconstituted wash solution is stable until the expiration date of the kit.
NS/0 HCP ELISA 試劑盒的使用注意事項
1 只用于研究或制造。
2 停止反應(yīng)試劑是0.5N 硫酸。避免接觸眼睛,皮膚,和服裝
3 本試劑盒所用的標準包含來自人血清免疫球蛋白。源材料進行了測試,發(fā)現(xiàn)有艾滋病和乙型肝炎表面抗原的抗體。沒有任何已知的方法可以使HIV或肝炎病毒或其他病原體總保證提供,因此處理這些試劑,好像他們是潛在的傳染。在用本試劑盒的試劑濃度,其他沒有被認為是有害的。
4 本試劑盒只能由合格的技術(shù)人員使用。
一切干細胞實驗耗材優(yōu)惠盡在華雅思創(chuàng)生物
2015年統(tǒng)統(tǒng)*,--一切活動盡在上海華雅思創(chuàng)!
:,客服
StemRD MGro-500 MSC medium,chemically-defined,間充質(zhì)干細胞培養(yǎng)基 500mL/瓶
LONZA 04-418Q X-VIVO 15 無血清培養(yǎng)基 1L/瓶
Life Technology 087-0112DK AIM-V® CTS™ 無血清細胞培養(yǎng)基(細胞治療級) 1000mL/瓶
Pall 15950-017 Ultroser Serum Substitute 血清替代品 20mL/瓶
LONZA 12-725F UltraCULTURE Serum-free Medium無血清培養(yǎng)基 500mL/瓶
BD 762165 PAXgene Blood RNA Tube RNA樣本收集管 100管/盒
LONZA 50150 SeaKem® Gold Agarose 金瓊脂糖粉 125g/瓶
LONZA CC-3162 EGM-2 BulletKit( CC-3156 & CC-4176 )套裝 Kit/套
Nunc 159910 175cm2易用培養(yǎng)瓶,PS,已滅菌,瓶蓋:過濾蓋 30個/箱
LONZA LT07-318 MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 100Tests/盒
LONZA LT07-418 MycoAlert® Mycoplasma Detection Kit支原體檢測試劑盒 50Tests/盒
Lonza 50-650U Kinetic-QCL 192 Test Kit內(nèi)毒素檢測試劑盒 192T/盒
Corning 431080 175cm⒉細胞培養(yǎng)瓶,Angled頸,透氣蓋 50個/箱
Corning 8388 125mL儲液瓶 Storage Bottles,PS,45mm瓶頸,帶蓋,滅菌,獨立包裝 24個/箱
Corning 430659 2ml凍存管, 聚丙烯,外旋蓋, 可自立圓底 500個/箱
Corning 3290 75cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 100個/箱
Corning 3292 175cm2 CellBIND Surface培養(yǎng)瓶,已滅菌,透氣蓋 50個/箱
BD-Falcon 352360 Cell Strainer, 100µm, Yellow 100um細胞濾網(wǎng) 50個/箱
BD Falcon 352070 50mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD Falcon 352096 15mL錐形離心管,高透明度聚丙烯,袋裝 500支/箱
BD BioCoat 354234 Matrigel Basement Membrane Matrix(標準型) 10mL/瓶
Cygnus F550 CHO HCP ELISA Kit,3G宿主蛋白檢測試劑盒 96T/盒
Cygnus F410 E.coli HCP ELISA Kit 大腸桿菌 96T/盒
Nalgene 5100-0001 Cryo 1℃ Freezing Container梯度降溫凍存盒 1個/箱
Nalgene 5026-0909 CryoBoxes 凍存盒 9X9 陣列,管瓶1.2/2.0mL 24個/箱
Nalgene 2250-0130 50L 可高溫高壓操作的細口大瓶(帶手柄) 1個/箱
Nalgene 2319-0050 20L 可高溫高壓滅菌的細口大瓶(帶放水口) 1個/箱
Hyclone SH30084.03 FBS 優(yōu)等澳洲胎牛血清 500mL/瓶
PeproTech 100-18B-500 Recombinant Human FGF-basic (154 a.a.)因子 500ug/支
Parafilm PM-996 Parafilm® M Laboratory Film 封口膜 12卷/箱