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REPOTMNFAT-GFP報(bào)告基因
REPOTMNFAT-GFP報(bào)告基因是吉滿(mǎn)生物自主研發(fā)的用于檢測(cè)NFAT轉(zhuǎn)錄活性水平為目的的報(bào)告基因。NFAT(Nuclear factor of activated T-cells)轉(zhuǎn)錄因子家族對(duì)細(xì)胞因子基因和其它一些對(duì)免疫反應(yīng)很關(guān)鍵的基因轉(zhuǎn)錄中起關(guān)鍵作用。
REPOTMNFAT-GFP報(bào)告基因主要用于檢測(cè)細(xì)胞PKC/Ca++信號(hào)通路中NFAT的轉(zhuǎn)錄活性、藥物研究以及基因過(guò)表達(dá)和RNAi的表型分析等。
pGMNFAT-GFP是吉滿(mǎn)生物改造后的哺乳動(dòng)物真核表達(dá)載體,在其多克隆位點(diǎn)插入了多個(gè)NFAT結(jié)合位點(diǎn),可以高效地檢測(cè)NFAT的激活水平。由于載體采用了GFP作為報(bào)告基因,更便于后續(xù)的檢測(cè)。同時(shí),對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外,由于質(zhì)粒體積減小,使得NFAT-GFP報(bào)告基因更易于轉(zhuǎn)染。
質(zhì)粒圖譜:
包裝清單:
產(chǎn)品編號(hào) | 產(chǎn)品名稱(chēng) | 包裝 |
GM-024012 | REPOTMNFAT-GFP報(bào)告基因 | 1μg |
- | 說(shuō)明書(shū) | - |
使用說(shuō)明:
pGMNFAT-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
注意事項(xiàng):
本質(zhì)粒未經(jīng)吉滿(mǎn)生物允許不得用于任何商業(yè)用途,也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康,實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。
保存條件:
-20℃保存。
參考文獻(xiàn):
[1] Herum KM, Lunde IG, Skrbic B, Florholmen G, Behmen D, Sjaastad I, Carlson CR, Gomez MF, Christensen G.Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress.J Mol Cell Cardiol. 2013 Jan;54:73-81. doi: 10.1016/j.yjmcc.2012.11.006. Epub 2012 Nov 22.
[2] Awla D, Zetterqvist AV, Abdulla A, Camello C, Berglund LM, Spégel P, Pozo MJ, Camello PJ, Regnér S, Gomez MF, Thorlacius H.NFATc3 regulates trypsinogen activation, neutrophil recruitment, and tissue damage in acute pancreatitis in mice.Gastroenterology. 2012 Nov;143(5):1352-60.e1-7. doi: 10.1053/j.gastro.2012.07.098. Epub 2012 Jul 27.
[3] Facundo HT, Brainard RE, Watson LJ, Ngoh GA, Hamid T, Prabhu SD, Jones SPO-GlcNAc signaling is essential for NFAT-mediated transcriptional reprogramming during cardiomyocyte hypertrophy.Am J Physiol Heart Circ Physiol. 2012 May 15;302(10):H2122-30. doi: 10.1152/ajpheart.00775.2011. Epub 2012 Mar 9.
[4] Finsen AV, Lunde IG, Sjaastad I, Østli EK, Lyngra M, Jarstadmarken HO, Hasic A, Nygård S, Wilcox-Adelman SA, Goetinck PF, Lyberg T, Skrbic B, Florholmen G, Tønnessen T, Louch WE, Djurovic S, Carlson CR, Christensen G.Syndecan-4 is essential for development of concentric myocardial hypertrophy via stretch-induced activation of the calcineurin-NFAT pathway.PLoS One. 2011;6(12):e28302. doi: 10.1371/journal.pone.0028302. Epub 2011 Dec 2.
[5] Minematsu H, Shin MJ, Celil Aydemir AB, Kim KO, Nizami SA, Chung GJ, Lee FY.Nuclear presence of nuclear factor of activated T cells (NFAT) c3 and c4 is required for Toll-like receptor-activated innate inflammatory response of monocytes/macrophages.Cell Signal. 2011 Nov;23(11):1785-93. doi: 10.1016/j.cellsig.2011.06.013. Epub 2011 Jun 25.