產(chǎn)品簡介
詳細(xì)介紹
α-葡萄糖苷酶(酵母麥芽糖酶)
外觀:液體(硫酸銨懸浮液)
在水中的溶解性:易溶
pH值:6.0
氣味:無
形狀:粉末
保質(zhì)期:在冰箱里10年以上,4°C保存,4年以上.
成分:
名稱 比例
α-葡萄糖苷酶(麥芽糖)(酵母) 100%
EC編號:3.2.1.20
基板 酶實測 比活度
(U / mg蛋白)
p一NP--葡萄糖苷 α-葡萄糖苷酶 76.0
p一NP-β-葡萄糖苷酶 β-葡萄糖苷酶 <0.001
p一NP-1-半乳糖 β-半乳糖苷酶 <0.001
p一NP-β-半乳糖 β-半乳糖苷酶 <0.001
封鎖的P -NP-Maltoheptoaside α-淀粉酶 <0.001
α-Glucosidase (yeast maltase)
EC 3.2.1.20
From yeast. Electrophoretically homogeneous. Ammonium sulphate suspension.
Specific activity: > 80 U/mg (40°C, pH 7.0, maltose as substrate).
Contaminants: α-amylase < 0.001%; ß-glucosidase < 0.001%.
Store at 4°C.
Stable for > 4 years at 4°C.
Catalogue Number: E-MALTS
Content: 2,000 Units
Appearance Powder
Specific Gravity ~ 1.5
Solubility in Water Readily soluble
pH Value 6.0
Odour None
Form Powder
Stability Stable in a refrigerator for ten or more years
Ingredients Name CAS Proportion
a-Glucosidase (Maltase) (yeast) 100%
PROPERTIES
1. ELECTROPHORETIC PURITY
- Single major band on SDS-gel electrophoresis (52,000)
- Single major band on isoelectric focusing (pI = 5.7)
2. SPECIFIC ACTIVITY AND LEVEL OF OTHER ACTIVITIES
SUBSTRATE ENZYME MEASURED SPECIFIC ACTIVITY
(U/mg protein)
p-NP-a-Glucoside a-Glucosidase 76.0
p-NP-b-Glucosidase b-Glucosidase < 0.001
p-NP-a-Galactoside b-Galactosidase < 0.001
p-NP-b-Galactoside b-Galactosidase < 0.001
Blocked p-NP-Maltoheptoaside a-Amylase < 0.001
All activities were measured at pH 6.8 and 40°C. Gylcosidase activities were measured using
the appropriate p-nitrophenyl glycoside (at 10 mM ). a-Amylase was measured using the
“CERALPHA” a-amylase assay reagent. One Unit of enzyme activity is the amount of enzyme
required to release one μmole of p-nitrophenol per min from the appropriate substrate
under the defined assay conditions.
3. PHYSICOCHEMICAL PROPERTIES
pH optima 6.4-6.8 pH Stability 5.6-7.
Temperature Optima 40°C Temperature Stability < 40°C
4. STORAGE CONDITIONS
The enzyme is supplied as an ammonium sulphate suspension in the presence of
0.02 % sodium azide and should be stored at 4°C. On diluting in buffer or water, the
enzyme should be stored in the frozen state.
產(chǎn)品關(guān)鍵詞:α-葡萄糖苷酶(酵母麥芽糖酶)