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        1. 上海北諾生物科技有限公司

          中級(jí)會(huì)員·13年

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          15800960770

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          克隆載體 pEGM-3ZF(+) A+,pEGM-11ZF(+) A+
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          中級(jí)會(huì)員·13年
          聯(lián)人:
          周經(jīng)理 劉經(jīng)理
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          產(chǎn)品簡(jiǎn)介
          可提供近萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動(dòng)物熒光載體,真核哺乳動(dòng)物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌
          產(chǎn)品介紹

          更多詳情咨詢(xún)    www.bnbiotech.com, ,

           

          下面所有質(zhì)粒和菌株,均為本公司實(shí)驗(yàn)室親自驗(yàn)證過(guò),可以提供,載體來(lái)源于promega,addgene,clontech,可提供質(zhì)粒圖譜,可提供近萬(wàn)種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純,pQE 載體,原核表達(dá)載體,克隆載體 ,畢赤酵母表達(dá)載體,畢赤酵母宿主菌,釀酒酵母表達(dá)載體 ,釀酒酵母宿主菌,真菌表達(dá)載體,真核哺乳動(dòng)物熒光載體,真核哺乳動(dòng)物載體,植物表達(dá)載體,大腸桿菌宿主菌,枯草宿主菌

           

          釀酒酵母表達(dá)載體

           

          pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF,pY15TEF,pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109;質(zhì)粒pGADT7,pGBKT7;對(duì)照質(zhì)粒pGBKT7-53pGBKT7-lam,pGADT7-TPCL1,釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc,配套畢赤酵母Pichiapink,畢赤酵母宿主X33,KM71,KM71HGS115,

           

           

           

          原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列,pET-GST,pGEX系列GST標(biāo)簽pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列,pQE系列,pTrc99a,pTrcHis系列,pBV220,221,222,pTXB系列,pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大腸桿菌冷激質(zhì)粒: pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒:pACYCduet-1,pETduet-1,pCDFduet-1,pRSFduet-1 Takara公司大腸桿菌分子伴侶: pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞: DH5a JM101 JM103 JM105 JM107 JM109 JM110 *0 *0F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a(pir) Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

           

           

           

          植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體隨機(jī)突變體庫(kù))pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1

           

           

           

          枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01, 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33

           

           

           

          RNAi基因沉默干擾敲除載體pSilencer1.0,pSilencer 2.1-U6 hygro pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo, pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體(oligoengine) pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide) RNAi慢病毒載體(addgene): pLKO.1

           

           

           

          基因cDNA文庫(kù)ORF表達(dá)克隆質(zhì)粒,

           

          擁有目前中國(guó)zui大的cDNA克隆庫(kù)、覆蓋10萬(wàn)人、鼠全長(zhǎng)基因的cDNA克隆和超過(guò)25,000個(gè)ORF克隆表達(dá)載體。

           

           

           

          哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2pcDNA3.1(-)/myc-His A ,pcDNA6-Myc/His B,pCEP4 pIRES,pIRESneo,pIRES hyg3pCMV-myc,pCMV-HApIRES-puro3,pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACT,pBINDpACT-MyoD,pBIND-Id,pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI ,pOPI3CAT,pCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)(Invitrogen)pcDNA4/TO/Myc-His ApcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His CpcDNA4/TO/Myc-His/LacZ,pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)(Clontech)pTet-OnpTet-Off,pTRE2,pRevTREpRevTet-On,pRevTet-off信號(hào)通路報(bào)告載體:pGAS-TA-LucpSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75;p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,pFA2-Elk1pFC-MEKK,pFR-luc,Gateway系統(tǒng)(invitrogen)pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR

           

           

           

          乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株,pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523,pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii

           

           

           

          廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1,

           

           

           

          腺病毒載體/慢病毒,逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒,腺病毒系統(tǒng)(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)(Stratagene) pAAV-MCS,pAAV-RCpHelper,pAAV-LacZpAAV-IRES-hrGFP,pCMV-MCS, 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝:二質(zhì)粒包裝系統(tǒng)一: psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二: pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率: pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng): PLP1 PLP2 PLP/VSVG

           

           

           

          基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽(yáng)性菌基因敲除,pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253,PL451,PL452,PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374

           

           

           

          熒光蛋白表達(dá)載體熒光定位質(zhì)粒報(bào)告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動(dòng)子增強(qiáng)子報(bào)告載體(Promega,帶有Luc熒光報(bào)告基因 pGL3-basic,pGL3-control,pGL3-enhancerpGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信號(hào)通路報(bào)告載體(Clontech) pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP pNFAT-TA-LucpCaspase3-sensor,pAP1(PMA)-Luc 信號(hào)通路報(bào)告載體(Promega)pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信號(hào)通路報(bào)告載體(Stratagene) p53-LucpAP-1-Luc,pNF-κB-LucpSRE-Luc, pFA2-Elk1pFC-MEKK,pFR-luc

           

           

           

          昆蟲(chóng)表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His,

           

           

           

          Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反轉(zhuǎn)錄酶MMLV,Klentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000質(zhì)粒

           

           

           

          BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155,pBiFC-VN173,pBiFC-bFosVC155,pBiFC-bFos(deltaZIP)VC155,pBiFC-bjun-VN173pCE-BiFC-VN173,pCE-BiFC-VC155,

           

           

           

          真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等,

           

           

           

          酵母單雜交三雜交載體,酵母雙雜交系統(tǒng)pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam,配套釀酒酵母AH109 酵母單雜交系統(tǒng)(Clontech):pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System(Stratagene): pSos, pSos MAFB, pMyr

           

           

           

          噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv 質(zhì)粒:pCANTAB5E 宿主菌:TG1 輔助噬菌體:M13K07 可溶表達(dá)菌:HB2151 2.噬菌體展示抗體Fab 質(zhì)粒:pcomb3 宿主菌:XL1-Blue 輔助噬菌體:VCSM13

           

          pEVOL,pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B,pCMV-N-HA,pUAST,pw-25,PAC-5.1-HISA,pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ,pORF-TK,pCOX2-Luc,pcDNA3.1(-)-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHC Promoter,Marc-145猴腎細(xì)胞,Sf9BmN昆蟲(chóng)家蠶細(xì)胞,酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌,pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1R1601,AGL1農(nóng)桿菌感受態(tài),原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,Rosetta gamiB(DE3)pLysS,TKB1,ER2566DB3.1,JM105,*0,DH5a,DH5alpha lambda pir,C43(DE3)菌株感受態(tài),pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139、pOJ260、pWHM3,pSET152,pVA981,多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917,pRK2013pVLT33,pMAD,RN4220金葡菌株,真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,

          pET載體

          3a, 3d

          3c-sumo

          5b

          11a A+

           

          12a A+

          15b A+

          16b A+

          17b A+

          19b A+

          20b A+

          21a,21b,21d A+

          22b A+

          23a,23b A+

          23a-tev

          24a K+

          25b K+

          26b K+

          27b K+

          28a,28b,28c K+

          28a-sumo

          28a-p53

          29a K+

          30a ,30c K+

          31b A+

          32a A+

          32a pelB A+

          35b K+

          39b K+

          40b K+

          41a,41b k+

          42a,42b K+

          43b A+

          43.1a A+

          44a A+

          48b K+

          49b K+

          50b K+

          pETduet-1

          pACYCduet-1

          pCDFduet-1

          pRSFduet-1

          pColAduet-1

          pET His

          pET DsbA

          pET GST

          pET Trx

          pQE載體

          PQE2

          pQE9

          pQE30,31,32,

          pQE30UA

          pQE30LacIq

          pQE40L

          pQE60

          pQE70

          pQE80L

           

          其他原核表達(dá)載體

          PBBR1 MCS Cm+

          PBBR1 MCS-2 K+

          PBBR1 MCS-3 Tet+

          PBBR1 MCS-4 A+

          PBBR1 MCS-5 慶大霉素

           

           

          pMal c2x,p2x,5x,5E

          pBV220,221,222

          pTrc99a,pTrcCKSpTrcHis A,B,C

          pBAD24,33,43,pBADHis A,B,C,pBADmycHisA

          pEZZ18

          pThio HisA A+

          pGEX4T-1,4T-2,4T-35X-1,6p-1,2t,3x

          pColdI

          pColdII

          pColdIII

          pColdTF

          pTwin1

          pRsetA,B,C

          PLLP-STQ

          pIN-ompA

          pkk223-3

          pSE380

          pJLA502

          pMBP-C,pMBP-p

          pTYB11,pCYB1

          Origene-N-His GST A+

          pPin point CAT A+

          pPin point xa1 A+

          pPin point xa2 A+

          pPin point xa3 A+

          pCW ori

           

          、

           

          常見(jiàn)克隆載體

          puc18,puc19,puc118,puc119

          pBlue Script SK+,

          pBlue ScriptSK+

          pEGM-3ZF(+) A+

          pEGM-11ZF(+) A+

          pACYC184

          pBR322

           

          基因敲除

          pKD3,pKD4,pKD46,pcp20

          pK18mobSacB

           

          海腎熒光素酶報(bào)告基因

          pGL3basic,promotor,enhancer,control

          pGL3 CM

          pGL4.29,4.30

           

           

          畢赤酵母表達(dá)載體

          pPIC9

          pPIC9k

          pPIC9k-1

          pPIC9k-His

          pPIC3.5

          pPIC3.5k

          pAO815

          pHIL-S1

          pPICZalphaA,BC

          pPICZalpha D

          pPICZalpha E

          pPICZalpha GA,GB

          pPICZalpha FC

          pPICZA,B

          pGAPZalphaA,BC

          pMETalphaA,B,C

          pFLD-cat A+

          pFLD-cat Z+

          pFLD1 Z+

           

          畢赤酵母宿主菌

          JC220

          JC308

          GS200

          GS190

          GS115

          KM71

          KM71H

          Y1991

          SMD1168

          SMD1168H

          X33

           

           

           

          釀酒酵母表達(dá)載體

          pYES2,pYES2-flag

          pYES2NTA,NTB,NTC

          pYES3CT

          pYIP5,pYRP7,pADH2

          pRSH41,pDC315,pDF15,pUG6

          pESC-His,BFD14,BFD15,pDNR1,p334,pDR195

          pRS316,pRS316HA

          YEplac112,YCplac33,YCplac22-EGFP,

          YEplac195,YEpLac181,YIP211

          pYX212pYX212-VHB

           

          釀酒酵母宿主菌

          INSV-

          YPH499

          YM4271

          BY4741

          W303-1A

           

          真核哺乳動(dòng)物熒光載體

          pEGFP N1,N2,N3

          Origene GFP

          pLEGFP N1

          pEGFP C1,C3

          AIF-EGFP N1

          pECFP N1,C1

          pEYFP N1,C1

          pEYFP-golgi

          pEYFPmem

          pBFPN1

          FIV-BFP

          pDsred MonomerN1

          pDsRed1 mito

          pDsRed2 mito

          pDsRed N1,C1

          pDsRed2 N1,C1

          pDsRed2-ER

          mRFP

           

          真核哺乳動(dòng)物載體

          pIRES

          pIRES2-EGFP

          pADeasy-1

          pADeasy-1(Bj5183)

          pAdtrack

          pAdtrack CMV

          pShuttle

          pShuttle CMV

          pRL CMV

          pCMV-HA

          pVAX1

          pVAST

          pCI

          pCI neo+

          pCMV-myc

          pflag-CMV2

          pcDNA3.1 +

          pcDNA3.1 zeo+

          pcDNA3.1 neo+

          pcDNA3.1 MycHisA

          pcDNA3.1 MycHisB

          pcDNA3.1-HBV genome

          pcDNA3-mRFP

          Hsp70-pcDNA3.1

          pcDNA 4 Max B

          psuper GFP neo

          psuper Retro GFP neo

          pSilencer4.1CMV puro+

          pSilencer4.1CM neo+

          psuper GFP neo

          psuper retro GFP neo

          pdisplay

          pBud CE4.1

          ptet on, ptet off

          pRL-tk,pRL-CMV

           

           

           

          植物表達(dá)載體

          pcambia1301,1302,1303,1304,1305.2

                 2300,2301

                 3300

          pcambia1301(EHA105)

          pcambia1381Z,1391Z

          PBI121,221

          PBI221-GFP

           

          植物農(nóng)桿菌宿主

          EHA105,LBA4404

           

          植物RNAi載體

          PHANNIBALPKANNIBAL

           

          啟動(dòng)子檢測(cè)

          pEGFP-1

          pkk232-8

           

          分子伴侶:

          PGKJE8,PGro7,pTf16,pKJE7,pGTf2

           

          酵母雙雜交

          pGADT7,pGBKT7,pGADT7-T,pGBKlam,pGBK-53,pCL1

          AH109,Y187

           

          酵母單雜交

          pGAD53m,p53-blue,p53-His,pLacZ,pHispHisi-1,

           

           

          哺乳動(dòng)物雙雜交

          pBIND-GFP,pBIND-ID,pGAD424,pGBT9pG5lac,

           

           

          大腸桿菌宿主菌

          JM101,105,109,110

          HB101,K802,C600,TH1,AM1,W3110,DH10bac

          Y1089,Y1090,BL21,BL21(DE3),MG1655

          BL21(DE3)plysS,BL21AIBL21SI,BL21codon plus RIPL,BL21 trxB (DE3),BL21 star

          DH5α,DH5αλpir,JM109,JM109(DE3)*0,*0F,,K12DB3.1,XL1-blue,XL-10gold,BM25.8,SG1117Turbo,TG1TB1,C41(DE3)C43(DE3),Turner(DE3),M15,E2566,C2566,ER2529,JF1125,Bj5183,Stbl3,Stbl4

          HMS174(DE3), HMS174(DE3)plysS, AD494(DE3)

          Novablue(DE3),Rosettalblue(DE3)

          Rosetta(DE3),Rosetta(DE5),Rosetta(DE3)plysS,

          Orgami(DE3),OrgamiB(DE3),Orgami(DE3)plysS

          Rosetta-gami2(DE3)plysS,Rosetta-gami(DE3),

          Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysS, Rosetta-gami(DE3)placI

           

           

           

           

          枯草表達(dá)系統(tǒng)pMA5,pHCMC05,pGFP22,pGFP315,pHP13,pHP13-43,pHY-43,pWB980,pAOX1

          枯草宿主菌:BS168DB403,BCL,1A75,WB600,WB800N

           

          昆蟲(chóng)表達(dá)系統(tǒng):pfastBacI,pfastII,pfastIII,pfastBacHTApfastBacHTB,pfastBacHTCpfastBacdual

           

          巨大芽孢桿菌系統(tǒng):pHis1525

          巨大宿主菌:YYBM1,WH320

           

          乳酸球菌表達(dá)系統(tǒng)  pMG36e,NZ9000,MG1363

           

          乳酸酵母表達(dá)系統(tǒng)  pKLAC1,GG799

           

          白色念珠菌系統(tǒng)pCR4,pCR4-GFP,p5921

           

          釀酒酵母表面展示系統(tǒng):pYD1,EBY100

           

          標(biāo)準(zhǔn)微生物菌株

          ATCC27853 銅綠假單胞菌

          ATCC29212 糞鏈球菌

          ATCC29213 金黃色葡萄球菌

          ATCC35218 大腸桿菌

          ATCC 35098四聯(lián)球菌

          NRRL Y-12968熱帶假絲酵母

          NRRL Y-2021德巴利酵母

          NRRL Y-1603畢赤氏巴斯德酵母

          DSM3586野油菜黃

           

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