PDPH，MBS，LC-SPDP，GMBS 雙功能交聯(lián)劑

PDPH，MBS，LC-SPDP，GMBS 雙功能交聯(lián)劑

產(chǎn)品名稱：GMBS

中文名稱：4-馬來酰亞胺基丁酸-N-琥珀酰亞胺酯

英文名稱：4-Maleimidobutyric acid N-hydroxysuccinimide ester

CAS #: 80307-12-6

簡介：GMBS is a non-cleavable heterobifunctional protein crosslinker. The GMBS crosslinking reagent is commonly used to crosslink haptens to carrier proteins and enzymes to antibody. The GMBS has a spacer arm length of 7.3 angstroms and is less immunogenic than the crosslinking analog MBS. The GMBS crosslinking reagent's NHS ester and maleimide reactive groups react with amino and sulfhydryl moites, respectively.

刺激是不可切割的異型蛋白交聯(lián)劑。的刺激是的交聯(lián)劑交聯(lián)的半抗原載體蛋白和抗體。的刺激有7.3埃的間隔臂的長度和較低的原性的化交聯(lián)。交聯(lián)劑的刺激的NHS酯和馬來酰亞胺基團(tuán)的活性氨基和巰基moites反應(yīng)。

Application

A heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thioether linkage buffered at pH 6.8 (6.5-7.0). Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. Provides a 7-atom linker.

結(jié)構(gòu)式：

Molecular Weight: 280.2

分子式 C12H12N2O6

CAS #: 80307-12-6

Spacer Length: 7.3 Å

Alternative Name: 4-Maleimdobutyric acid N-succinimidyl ester

純度：98%+

保存：-20℃

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產(chǎn)品名稱：LC-SPDP

英文全稱：(succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate)

General description

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.

我們試驗試劑胺活性部分N-羥基琥珀酰亞胺（NHS）酯。反應(yīng)通常在磷酸鹽、碳酸鹽/碳酸氫鹽或硼酸鹽緩沖液中進(jìn)行。如果不含伯胺（或硫醇或二硫鍵還原試劑）-見巰基反應(yīng)性，可以使用其他緩沖劑。反應(yīng)速率和水解降解率隨pH值的增加而增加；例如，ph值為7的ph值為9小時，pH值小于10分鐘。NHS酯試劑SPDP和lc-spdp有限的水溶解度和溶解在有機(jī)溶劑中之前，添加到反應(yīng)混合物。磺NHS酯試劑磺LC SPDP是水溶性的，可以直接加入到反應(yīng)混合物的水

簡介：LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm of SPDP.

結(jié)構(gòu)式：

外觀：白色干燥粉末

Molecular Weight: 425.5

分子式C18H23O5N3S2

溶解性：DMSO or DMF

Spacer Length: 15.7 Å

Alternative Name: Succinimidyl-6-(3-[2-pyridyldithiol-propionamido) hexanoate

純度：98%+

保存：-20℃

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產(chǎn)品名稱：MBS

英文名稱：3-Maleimidobenzoic acid N-hydroxysuccinimide ester

中文名稱：3-馬來酰亞胺基苯甲酸琥珀酰亞胺酯

CAS #: 58626-38-3

簡介：MBS crosslinker is a non-cleavable, water insoluble, heterobifunctional protein crosslinking rea-gent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody. The MBS crosslinking reagent's NHS ester and maleimide reactive groups react with amino and sulfhydryl moites, respectively.

對伯胺和巰基具有反應(yīng)性的雙異官能化交聯(lián)劑。通常，初始反應(yīng)在 pH 為 7.0 時，通過酰胺鍵偶聯(lián)含伯胺的分子，然后在相同 pH 通過硫醚偶聯(lián)含巰基的化合物。用于制備交聯(lián)劑和半抗原載體分子偶聯(lián)物。

結(jié)構(gòu)式：

Molecular Weight: 314.3

分子式：C15H10N2O6 

CAS #: 58626-38-3

Spacer Length: 7.3 Å

純度：98%+

保存：-20℃

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產(chǎn)品名稱：PDPH

英文全稱：(3-(2-pyridyldithio)propionyl hydrazide)

簡介：PDPH is a cleavable, heterobifunctional protein crosslinking reagent. The PDPH crosslinker's hydrazide group selectively reacts with oxidized carbohydrates (or carboxyls when used with EDC); while the pyridinyldisulfide moiety is reactive toward reduced thiols. Thus, PDPH protein crosslinker is an excellent choice for crosslinking glycoproteins and other polysaccharides.

PDPH是裂解，異型雙功能蛋白交聯(lián)試劑。PDPH的酰肼基團(tuán)的交聯(lián)劑的反應(yīng)選擇性氧化碳水化合物（或羧基與使用EDC）；而pyridinyldisulfide部分活性對降低硫醇。因此，蛋白質(zhì)交聯(lián)劑交聯(lián)PDPH的糖蛋白和多糖等的一個的選擇。

General description

The PDPH is a heterobifunctional crosslinker containing sulfhydryl-reactive pyridyldithiol and carbonyl-reactive hydrazide moieties. Pyridyldithiols react with free sulfhydryls (-SH) to form disulfide bonds. Hydrazide groups react with carbonyls (aldehydes and ketones) to form stable hydrazone bonds. Aldehyde groups can be created by periodate-oxidation of sialic acid and other sugar components of glycoprotein polysaccharides. Thus, PDPH is useful for conjugating glycoproteins and sulfhydryl-containing peptides or proteins. Likewise, PDPH is useful as a sulfhydryl-addition reagent for glycoproteins and other carbohydrates; after reaction of the hydrazide to an oxidized carbohydrate, the pyridyldithiol group can be cleaved by a reducing agent to expose a sulfhydryl group. Yet another application for PDPH is to react the primary amine of the hydrazide moiety to a carboxyl group using the crosslinker EDC.

結(jié)構(gòu)式：

外觀：白色粉末   

Molecular Weight: 229.3

分子式：C8N3S2OH11 

溶解性：DMSO or DMF

Spacer Length: 9.2 Å

Alternative Name: 3-[2-Pyridyldithio]propionyl hydrazide

純度：98%+

保存：-20℃

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Features and Benefits

• Reactive groups: pyridyldisulfide and hydrazide

• Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups

• Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)

• Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents

• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates

• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups

• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

產(chǎn)品名稱：SBA

英文名稱：Bromoacetic Acid N-Succinimidyl Ester

中文名稱：溴乙酸-N-琥珀酰亞胺酯

CAS #: 42014-51-7

簡介：SBA crosslinker (N-Succinimidyl bromoacetate) is a sulfhydryl (thiol) and amino reactive hetero-bifunctional protein crosslinking reagent. SBA crosslinker is non-cleavable and is among the shortest amine and sulfhydryl reactive crosslinking reagents known with a spacer arm length of only 1.5 Angstroms.

該交聯(lián)劑（溴酯）是一種巰基（硫醇）和氨基反應(yīng)異型雙功能蛋白交聯(lián)試劑。該交聯(lián)劑是非裂解和在短的氨基和巰基交聯(lián)試劑與一個只有1.5埃的間隔臂的長度已知。

A heterobifunctional cross-linking reagent which allows bromoacetylation of primary amine groups followed by coupling to sulfhydryl-containing compounds. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. The second reaction results in thioether bonding in pH range 7.0-8.0.

外觀：白色粉末

Molecular Weight: 236.02

分子式:C6H6BrNO4

CAS #: 42014-51-7

Spacer Length: 1.5 Å

Alternative Names: Bromoacetic acid N-hydroxysuccinimide ester;

純度：98%+

保存：-20℃

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產(chǎn)品名稱：SIA

英文名稱：N-Succinimidyl Iodoacetate

中文名稱：碘乙酸 N-羥基琥珀酰亞胺酯

CAS #: 39028-27-8

簡介：N-Succinimidyl iodoacetate (SIA) is a non-cleavable, heterobifunctional protein crosslinker. SIA is among the shortest amine and sulfhydryl (thiol) reactive crosslinkers known with a spacer arm length of 1.5 Angstroms.

具有氨基和巰基反應(yīng)性的雙異官能團(tuán)交聯(lián)劑。情況下，初通過 pH 7.5 (6.5-8.5) 緩沖條件下的酰胺鍵偶聯(lián)到含有伯胺的分子上，接著通過 pH 7.5 (7.0-8.0) 緩沖條件下的硫醚鏈與含有自由巰基的分子進(jìn)行特異性次級鍵合。用于制備交聯(lián)劑和半抗原載體分子偶聯(lián)物。提供兩個原子的連接基。

外觀：白色粉末

Molecular Weight: 283.02

分子式:C6H6INO4

溶解性：DMF

CAS #: 39028-27-8

Spacer Length: 1.5 Å

Alternative Names: Iodoacetic acid N-hydroxysuccinimide ester; Succinimidyl iodoacetate

純度：98%+

保存：-20℃

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