BMOE，KMUH，EMCH，BMPH，TSAT

BMOE，KMUH，EMCH，BMPH，TSAT

產(chǎn)品名稱：SPDP

英文名稱：N-Succinimidyl 3-(2-Pyridyldithio)propionate

中文名稱：3-(2-吡啶基二硫基)丙酸N-羥基琥珀酰亞胺酯

CAS #: 68181-17-9

簡介：SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive heterobi-functional protein crosslinker. The SPDP crosslinking reagent's reactive moites consist of an N-hydroxysuccinimide (NHS) ester that reacts with primary amines. On the other end of SPDP, the pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

SPDP交聯(lián)劑是可分解的，不溶于水，氨和硫醇（巰基）反應(yīng)heterobi功能蛋白交聯(lián)劑。SPDP交聯(lián)試劑的反應(yīng)的moites由N-羥基琥珀酰亞胺（NHS）酯與伯胺反應(yīng)。在另一端的pyridinyldisulfide SPDP，反應(yīng)sulhydryls產(chǎn)生可逆的二硫鍵。

Application

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is useful for the preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.

It has been used for antibody conjugation and as a protein cross-linker.

General description

3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester (SPDP) is a heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. It is typically coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thiol-disulfide exchange buffered at pH 8.0 (7.5-8.5). Alternay, this reagent may be used as a protected thiolating reagent following initial coupling and reduction of linker. It incorporates four atom linker.

外觀：白色粉末

Molecular Weight: 312.4

分子式:C12H12N2O4S2

CAS #: 68181-17-9

溶解：DMSO or DMF

Spacer Length: 6.8 Å

Alternative Name: 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

純度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

產(chǎn)品名稱：Sulfo-LC-SPDP

英文名稱：(sulfosuccinimidyl 6-[3′-(2-pyridyldithio)propionamido]hexanoate)

簡介：Sulfo-LC-SPDP crosslinking reagent is a cleavable, water soluble, amino and thiol (sulfhydryl) reactive heterobifunctional protein crosslinker.

Sulfo-LC-SPDP crosslinker's reactive moites consist of an N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines. The other end of the Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

LC SPDP交聯(lián)試劑磺可裂解、水溶性氨基和巰基反應(yīng)異型雙功能交聯(lián)劑（巰基）蛋白。

SPDP交聯(lián)劑的反應(yīng)moites磺LC組成的N-羥基（磺基NHS）酯與伯胺反應(yīng)。的LC SPDP交聯(lián)分子pyridinyldisulfide磺化反應(yīng)sulhydryls產(chǎn)生可逆的二硫鍵的另一端。

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

General description

The SPDP reagents are a unique group of amine- and sulfhydryl-reactive heterobifunctional crosslinkers. Whether they are used to form amine-to-amine or amine-to-sulfhydryl crosslinks among molecules, the SPDP reagents produce disulfide-containing linkages that can be cleaved later with reducing agents such as dithiothreitol (DTT).

外觀：白色粉末

Molecular Weight: 527.6

分子式：C14H16N3NaO8S3

溶解：水中

Spacer Length: 15.7 Å

Alternative Name: Sulfosuccinimidyl-6-(3’-[2-pyridyldithio]-propionamido) hexanoate

純度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

產(chǎn)品名稱：Sulfo-MBS

英文名稱：(m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester)

簡介：Sulfo-MBS crosslinker (m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester) is a non-cleavable, water soluble, heterobifunctional protein crosslinking reagent that is commonly used to crosslink haptens to carri-er proteins and enzymes to antibody. The Sulfo-MBS crosslinking reagent's NHS ester and maleimide reac-tive groups react with amine and sulfhydryl moites, respectively.

磺基交聯(lián)劑（MBS m-maleimidobenzoyl-n-hydroxysulfosuccinimide酯）是一種非裂解、水溶性、異型雙功能蛋白交聯(lián)劑是的交聯(lián)半抗原載體蛋白和抗體。磺基MBS交聯(lián)試劑的NHS酯和馬來酰亞胺的反應(yīng)性基團與氨基和巰基moites反應(yīng)

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

General description

MBS and its water-soluble analog Sulfo-MBS are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these crosslinkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneously with the maleimide (sulfhydryl-targeted) reaction.

外觀：白色粉末

Molecular Weight: 416.3

分子式：C15H9O9N2NaS

Spacer Length: 7.3 Å

Alternative Names: 3-Maleimidobenzoic acid N-hydroxysulfosuccinimide ester;

純度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司