膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
膜染料 DAPI Ex/Em (nm) 358/461
Overview | Printer Friendly Version |
Ex/Em (nm) | 358/461 |
MW | 350.25 |
CAS # | 28718-90-3 |
Solvent | Water |
Storage | F/D/L |
Category | Nucleic Acid Detection DNA Detection |
Related | Labeling Cells Fluorescence Imaging Biochemical Assays |
DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.
西安瑞禧生物科技有限公司是的熒光染料,我們提供的熒光染料的吸收波長和發(fā)射波長從300nm-810nm不等,我們可以再不同的熒光染料上面做的活性基團(tuán),比如NHS、amine、COOH、azide、alkyne、maleimide等等。關(guān)于熒光染料我們可以提供FITC、Rhodamine、Bodipy系列染料、Coumairn 染料、Dansyl染料、FAM/5-TAMRA、NBD、CY系列、ICG及IR系列、ATTO系列染料、AMCA、Perylene、碘化物、DIR/DIL/DIO/DID/DAPI膜染料等等。我公司特別在近紅外熒光染料Near infrared dyes方面有的產(chǎn)品線。
Bodipy系列染料(氟化硼二吡咯類熒光染料)
Bodipy氟化硼二吡咯類熒光染料相對比于熒光素、羅丹明或者菁染料有著較的量子產(chǎn)率、摩爾消光系數(shù)、的光學(xué)穩(wěn)定性和對PH不的被應(yīng)用于生物標(biāo)記、熒光探針及生物成像,西安瑞禧生物科技有限公司可以提供的Bodipy熒光染料。
瑞禧生物的BODIPY類熒光染料衍生物:
1:比50%
2:包裝量比大一倍
3:95%+
4:可以提供相關(guān)檢測圖譜及
5:如有質(zhì)量問題
以上資料源于西安瑞禧生物科技有限公司
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