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當(dāng)前位置:西安瑞禧生物科技有限公司>>技術(shù)文章>>幾十種熒光蛋白或活化基團(tuán)改性蛋白(蛋白)
幾十種熒光蛋白或活化基團(tuán)改性蛋白(蛋白)
西安瑞禧生物科技有限公司是可以提供一些列的蛋白類產(chǎn)品改性 熒光標(biāo)記,偶連抗體 多肽或者聚合物或其他小分子偶連蛋白產(chǎn)品,我們也可以把一些無機(jī)納米顆粒如納米金棒 磁性納米顆粒偶連蛋白。
我公司可以提供修飾改性熒光標(biāo)記的蛋白有以下:
BSA牛血清白蛋白, HAS人血清白蛋白, Streptavidins鏈霉親和素,Concanavalin A 刀豆球蛋白
Transferrin 轉(zhuǎn)鐵蛋白, Protein A 重組蛋白A, Protein G 重組蛋白G, Actin 肌動(dòng)蛋白, Histone 組蛋白, Ovalbumin 卵清蛋白, 乳清蛋白lactalbumin,Hemoglobin 血紅蛋白, Mucin 粘蛋白, Myoglobin 肌紅蛋白, Nucleohistone 核仁蛋白, Casein 絡(luò)蛋白,Lactoferrin乳鐵蛋白,Azo-Casein偶氮酪蛋白,Laminin 層粘蛋白,Keratin azure天青角蛋白,Protamine魚精蛋白,Ferredoxin 鐵氧還蛋白, Green fluorescent protein (GFP)綠色熒光蛋白
我們可以提供以下熒光染料標(biāo)記上述蛋白:
FITC/Rhodamine/TRITC/Comarin/Bodipy/ICG/CY3/CY3.5/CY5/CY5.5/CY7/CY7.5/IR-800/IR-825或其他系列IR熒光染料
對(duì)以上蛋白進(jìn)行活性基團(tuán)改性的基團(tuán)包括有:
活化脂NHS/氨基NH2/Biotin/疊氮N3/醛基CHO/巰基SH/馬來酰亞胺MAL/炔烴Alkyne/DBCO/Vinyl sulfone/DOTA/NOTA/TCO/Tetrazine
定制偶連的復(fù)合類產(chǎn)品包括有:
以上蛋白可以和納米金或納米金棒偶連或氧化硅納米顆粒偶連
以上蛋白可以和聚合物或者共聚物偶連
以上蛋白可以和點(diǎn)擊化學(xué)類產(chǎn)品或大環(huán)類小分子偶連
以上蛋白可以和其他生物活性產(chǎn)品交聯(lián) 如抗體 多肽 或活性
以上蛋白可以根據(jù)文獻(xiàn)制備定制類產(chǎn)品
【熒光標(biāo)記人/牛血清白蛋白】:
Bovine serum albumin (BSA) is a slightly acidic ~66 kd protein. BSA is commonly used in biological research due to its low cost and inertness. Dilute enzymes can be stabilized by BSA (useful for restriction digests). BSA is a common blocking agent in western blotting and ELISA’s. Although, bovine serum albumin is exceptionally soluble (up to 50% w/v in buffered saline), it is typically used at a concentration of 0.1-1% w/v.
產(chǎn)品介紹:
牛血清白蛋白,英文名稱為Bovine Serum Albumin,也稱Bovine albumin,或Cohn Fraction V,簡稱BSA。BSA有著其的實(shí)驗(yàn)室應(yīng)用,在實(shí)驗(yàn)中作封閉劑,包括ELISA、WB(Western Blot)。作為載體蛋白,將其交聯(lián)于半抗原和其他弱抗原可以使它們?cè)诳贵w中具有的原性。在限制性內(nèi)切消化反應(yīng)中,BSA常常被用作一些的反應(yīng)穩(wěn)定劑,并且能防止它粘附到管壁和槍頭上。BSA也于生物制的過程或者作為營養(yǎng)物用于和微生物培養(yǎng)。除此之外,還作為蛋白定量檢測的標(biāo)準(zhǔn)品使用。我們提供的BSA產(chǎn)品,使用牛血漿,利用熱休克法(Heat Shock)法制備而成。
牛血清白蛋和人血清白蛋白在靶向和傳遞方面的介紹:
與正常組織相比,組織的,結(jié)構(gòu) 一個(gè)的表現(xiàn)就是內(nèi)皮間隙,這種變情況下大分子可以透過組織壁的原因,然而在健康組織的中只有小分子可以通過這一內(nèi)皮屏障,通常大分子流體動(dòng)力學(xué)半徑大于2nm小于10nm 人血清白蛋白的半徑是7.2nm 因此可以滲入組織而不能進(jìn)入正常組織。
在例條件下 在或轉(zhuǎn)移時(shí),變釋放的化學(xué)物質(zhì)和衍生物的甲狀腺相關(guān)肽均通過產(chǎn)生NO和KATP來降低集合淋巴管“節(jié)律泵”的活性,從而影響了淋巴的生成 轉(zhuǎn)運(yùn) 使得組織淋巴回流較小,分子量相對(duì)較小的物質(zhì)從間質(zhì)中較快,而大分子被截留,因此大分子物質(zhì)還可以在組織中選擇性的聚集。通透性和截留率是白蛋白作為抗靶向載體的條件,白蛋白的這種效應(yīng)符合大分子物質(zhì)在組織的透過性增及滯留效應(yīng)。
我公司可以提供白蛋白熒光探針和活化基團(tuán)改性的牛血清白蛋白和人血清白蛋白產(chǎn)品,并且可以做一些白蛋白復(fù)合定制類產(chǎn)品。
已有產(chǎn)品列表:
(BSA-FITC), Bovine serum albumin Fluorescein labeled 異硫氰酸熒光素標(biāo)記牛血清白蛋白
(BSA-TRITC), Bovine serum albumin TRITC labeled 四甲基落地那么標(biāo)記牛血清白蛋白
(BSA-CY3), Bovine serum albumin CY3 labeled
(BSA-CY5), Bovine serum albumin CY5 labeled
(BSA-CY5.5), Bovine serum albumin CY5.5 labeled
(BSA-CY7), Bovine serum albumin CY7 labeled
(BSA-CY7.5), Bovine serum albumin CY7.5 labeled
(BSA-ICG), Bovine serum albumin ICG labeled 吲哚菁綠標(biāo)記牛血清白蛋白-近紅外
(BSA-SH), Bovine serum albumin thiol labeled 巰基功能化牛血清白蛋白
(BSA-Biotin), Bovine serum albumin Biotin labeled 修飾牛血清白蛋白
(BSA-MAL), BSA, maleimide function 馬來酰亞胺功能化牛血清白蛋白
(BSA-LA), BSA, linoic acid labeled
(BSA-OA), BSA, oleic acid labeled 油酸包裹牛血清白蛋白
(BSA-N3), BSA, azide labeled 疊氮功能化牛血清白蛋白
IR825-BSA, Bovine serum albumin IR-825 labeled 近紅外染料標(biāo)記牛血清白蛋白
Gold-BSA, Bovine serum albumin Gold labeled 納米金偶連牛血清白蛋白
【熒光標(biāo)記和功能化人血清白蛋白】:
Ruixibio provides a variety of chemically functionalized and bioconjugated Human serum albumin (HSA) with different functionality. These functional HSA products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
(HSA-FITC), Human serum albumin Fluorescein 熒光素標(biāo)記人血清白蛋白
(HSA-TRITC), Human serum albumin TRITC 四甲基羅丹明標(biāo)記人血清白蛋白
(HSA-CY5), Human serum albumin CY5
(HSA-CY3), Human serum albumin CY3
(HSA-CY5.5), Human serum albumin CY5.5
(HSA-CY7), Human serum albumin CY7
(HSA-CY7.5), Human serum albumin CY7.5
(HSA-ICG), Human serum albumin ICG 吲哚菁綠標(biāo)記人血清白蛋白-近紅外
(HSA-SH), Human serum albumin thiol 巰基功能化人血清白蛋白
(HSA-Biotin), Human serum albumin Biotin 修飾人血清白蛋白
(HSA-MAL), HSA, maleimide function 馬來酰亞胺功能化人血清白蛋白
(HSA-IR825), Human serum albumin IR-825 近紅外染料標(biāo)記人血清白蛋白
(HSA-Gold), Human serum albumin gold
Human serum albumin (HSA, without modification)
【熒光標(biāo)記和功能化卵清蛋白】
蛋清中的主要蛋白質(zhì)包括卵清蛋白、卵轉(zhuǎn)鐵球蛋白、類卵沾蛋白、卵沾蛋白、溶菌、球蛋白G2、球蛋白G3等。卵清蛋白等電點(diǎn)4.5,其本質(zhì)是含磷糖蛋白。卵清蛋白是蛋清中的主要蛋白組分,其結(jié)構(gòu)與性質(zhì)會(huì)影響蛋清蛋白在加工過程中的功能性質(zhì)。因此研究卵清蛋白在熱處理或美拉德反應(yīng)后的變化對(duì)了解蛋清蛋白在加工過程中的結(jié)構(gòu)與性質(zhì)的變化有意義。
卵清蛋白是一種蛋白質(zhì),占蛋清蛋白總量的54%-69%,卵清蛋白是的球蛋白,分子量為44.5kDa,屬含磷糖蛋白,含有四個(gè)自由巰基、385個(gè)氨基酸殘基 [1] 。這些氨基酸殘基相互纏繞折疊形成具有度二級(jí)結(jié)構(gòu)的球型結(jié)構(gòu),大部分為α-螺旋和β-折疊。卵清蛋白有1個(gè)二硫鍵和4個(gè)巰基,當(dāng)加熱時(shí)暴露出來,通過分子間相互作用使卵清蛋白膠體結(jié)構(gòu)變得穩(wěn)定
Ovalbumin (abbreviated OVA) is the main protein found in egg white. Ovalbumin has been used in the study of protein structure, proteomics and immunology. Ruixibio provides a variety of functionalized and bioconjugated ovalbumin conjugates.These labeled ovalbumin products were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
產(chǎn)品列表:
FITC-Ovalbumin, Ovalbumin, FITC conjugate 綠色熒光標(biāo)記卵清蛋白
Rhodamine-Ovalbumin ,Ovalbumin, Rhodamine labeled 紅色熒光標(biāo)記卵清蛋白
CY3-Ovalbumin, Ovalbumin, CY3 conjugate
Cy5-Ovalbumin ,Ovalbumin, CY5 labeled 藍(lán)色熒光標(biāo)記卵清蛋白
Cy5.5-Ovalbumin ,Ovalbumin, CY5.5 labeled 紫色熒光標(biāo)記卵清蛋白
Cy7-Ovalbumin ,Ovalbumin, CY7 labeled
Cy7.5-Ovalbumin ,Ovalbumin, CY7.5 labeled
ICG-Ovalbumin ,Ovalbumin, ICG labeled 吲哚菁綠標(biāo)記卵清蛋白
Biotin-Ovalbumin ,Ovalbumin, Biotin function 修飾卵清蛋白
Ovalbumin-Thiol, Ovalbumin, Thiol function 巰基化卵清蛋白
Ovalbumin-MAL, Ovalbumin, maleimide function 馬來酰亞胺修飾卵清蛋白
【乳清蛋白lactalbumin】
該納米載體取材綠色、設(shè)計(jì)巧妙,可在部位依次針對(duì)表面過表達(dá)、微酸和谷胱甘肽(GSH)濃度等微實(shí)現(xiàn)三重響應(yīng)性釋(圖2)。作者先通過定向解奶酪副產(chǎn)物-乳清蛋白獲得兩親性多肽,在水溶液中可自組裝形成30納米單分散膠束peptosome (PS),荷載疏水性抗姜黃素Cur,了姜黃素水溶液難溶的問題,并降低其對(duì)正常組織的副作用。隨后他們對(duì)其表面修飾功能肽段PePm,其中Pe可靶向表面的ErB2受體,且兼具的抗轉(zhuǎn)移效果。此外,Pm片段可以根據(jù)表面過表達(dá)金屬基質(zhì)蛋白MMP的特異性水解,通過交聯(lián)單個(gè)載體內(nèi)部自身巰基形成二硫鍵,使PS具有GSH濃度響應(yīng)釋的功能。他們將所構(gòu)建的載體PePm/PS/Cur經(jīng)尾靜脈注射到小鼠體內(nèi)后,經(jīng)EPR效應(yīng)和Pe的靶向效應(yīng)在部位度富集,載體表面Pe肽被MMP水解脫離,使Pe肽留在表面發(fā)揮抑和抗轉(zhuǎn)移效果。PS/Cur繼續(xù)通過“質(zhì)子海綿”效應(yīng)從溶體中逃逸,在質(zhì)內(nèi)通過GSH響應(yīng)逐漸解體,釋放出PS/Cur內(nèi)荷載的姜黃素,發(fā)揮抑制的作用。
產(chǎn)品目錄:
FITC-lactalbumin, lactalbumin, FITC conjugate
Rhodamine-lactalbumin , lactalbumin, Rhodamine labeled
CY3-lactalbumin, lactalbumin, CY3 conjugate
Cy5-lactalbumin , lactalbumin, CY5 labeled
Cy5.5-lactalbumin , lactalbumin, CY5.5 labeled
Cy7-lactalbumin , lactalbumin, CY7 labeled
Cy7.5-lactalbumin , lactalbumin, CY7.5 labeled
ICG-lactalbumin , lactalbumin, ICG labeled
Biotin-lactalbumin, lactalbumin, Biotin function
lactalbumin-Thiol, lactalbumin, Thiol function
lactalbumin-MAL, lactalbumin, maleimide function
【熒光標(biāo)記刀豆求蛋白A】:
豆球蛋白A(Con A),又名豆凝集素、豆素,是一種植物血凝素,具的有絲作用,有的淋巴轉(zhuǎn)化反應(yīng)的作用,其淋巴轉(zhuǎn)化適濃度為40-100μg/ml,能沉淀糖原,凝集羊、馬、狗、兔、豬、大鼠、小鼠、豚鼠等及人紅。還能選擇性抑制性T(Ts),對(duì)調(diào)節(jié)機(jī)體反應(yīng)具有作用。因此,通過使用Con A來活化態(tài)(或老年)時(shí)的Ts這一途徑,可望改觀一些自身性,甚或移植物排斥反應(yīng)或惡性的防治。
豆蛋白(Concanavalin A,Con A)是從豆(Jack bean)中提取出來的凝集素,能沉淀多種糖類,葡聚糖和果聚糖等很多其它多糖,以及球蛋白和血型物質(zhì)等多種糖蛋白,還沉淀炎球菌多糖,并能凝集多種紅。Con A 能和很多和反應(yīng),能區(qū)分某些正常和,能進(jìn)(有絲作用)。Con A IV 用于淋巴下?lián)苻D(zhuǎn)化實(shí)驗(yàn),也被用來刺激T產(chǎn)生IL-1 樣因子,還用于脾增生,研究所必需的配體/受體作用。此外,尚區(qū)分正常分泌ACTH 的垂體和分泌ACTH 的腺、用于研究糖基化在離子通道調(diào)節(jié)中的作用、用于糖蛋白的分離純化和多糖、糖蛋白糖鏈結(jié)構(gòu)的研究等。
1992 年,Tiegs 等地應(yīng)用豆蛋自A( ConA)誘發(fā)了小鼠特異性,這一實(shí)驗(yàn)?zāi)P褪瞧饋淼挠?/span>T 淋巴介導(dǎo)的損害,它的建立和應(yīng)用為研究損害的學(xué)與分子學(xué)機(jī)制以及進(jìn)行篩選提供了為和的實(shí)驗(yàn)?zāi)P汀?/span>
Concanavalin A (con A) is a homotetrameric, 26 kDa lectin derived from the jack bean, Canavalia ensiformis. Concanavalin A binds to alpha-D glucose and alpha-D-mannose containing saccharides. Concanavalin A requires both Mn2+ and Ca2+ ions as a co-factor for binding. Since each con A has four binding sites, it can agglutinate cells such as red blood cells. Con A can also cause cell surface receptor aggregation which can be mitogenic for some lymphocytes.
產(chǎn)品列表:
Concanavalin A-CY5
Concanavalin A-CY3
Concanavalin A-CY5.5
Concanavalin A-CY7.5
Concanavalin A-CY7
Concanavalin A-Biotin
Concanavalin A-SH
Concanavalin A-TRITC
Concanavalin A-FITC
Concanavalin A-ICG
【熒光標(biāo)記肌動(dòng)蛋白】
Actin is a major cell cytoskeleton microfilament protein that plays an important role in cellular functions such as cell mobility and contraction during cell division. Ruixibio provides a broad range of fluorescent or biotin labeled actin bioconjugates. These conjugates include coumarin labeled actin, fluorescein (FITC, FAM) actins, rhodamine (TRITC, TMR, TAMRA, rhodamine B) actins, cyanine dye (cy3, cy5, cy5.5, cy7) actins and many other actin conjugates.
Actin from rabbit muscle
FITC-Actin, Actin from rabbit muscle, Fluorescein labeled
TMR-Actin, Actin from rabbit muscle, tetramethyl rhodamine (TMR) labeled
TRITC-Actin ,Actin from rabbit muscle, Rhodamine B (TRITC) labeled
CY3-Actin ,Actin from rabbit muscle, Cy3 labeled
CY5-Actin ,Actin from rabbit muscle, Cy5 labeled
CY5.5-Actin ,Actin from rabbit muscle, Cy5.5 labeled
CY7-Actin ,Actin from rabbit muscle, Cy7 labeled
CY7.5-Actin ,Actin from rabbit muscle, Cy7.5 labeled
ICG-Actin ,Actin from rabbit muscle, ICG labeled
Biotin-Actin, Actin from rabbit muscle, Biotin labeled
【熒光標(biāo)記的組蛋白】:
FITC-Histone
Rhodamine-Histone
CY3-Histone
CY5-Histone
CY5.5-Histone
CY7-Histone
CY7.5-Histone
ICG-Histone
【熒光標(biāo)記血紅蛋白】:
Hemoglobin-CY5
Hemoglobin-CY3
Hemoglobin-CY5.5
Hemoglobin-CY7.5
Hemoglobin-CY7
Hemoglobin-Biotin
Hemoglobin-SH
Hemoglobin-TRITC
Hemoglobin-FITC
【熒光標(biāo)記粘蛋白】:
Mucin-CY5
Mucin-CY3
Mucin-CY5.5
Mucin-CY7.5
Mucin-CY7
Mucin-Biotin
Mucin-SH
Mucin-TRITC
Mucin-FITC
【熒光標(biāo)記肌紅蛋白】:
Myoglobin-CY5
Myoglobin-CY3
Myoglobin-CY5.5
Myoglobin-CY7.5
Myoglobin-CY7
Myoglobin-Biotin
Myoglobin-SH
Myoglobin-TRITC
Myoglobin-FITC
【熒光標(biāo)記核仁蛋白】:
Nucleohistone-CY5
Nucleohistone-CY3
Nucleohistone-CY5.5
Nucleohistone-CY7.5
Nucleohistone-CY7
Nucleohistone-Biotin
Nucleohistone-SH
Nucleohistone-TRITC
Nucleohistone-FITC
【熒光標(biāo)記轉(zhuǎn)鐵蛋白】:
轉(zhuǎn)鐵蛋白又名運(yùn)鐵蛋白 transferrin,TRF,siderophilin)是血漿中主要的含鐵蛋白質(zhì),負(fù)責(zé)運(yùn)載由消化管吸收的鐵和由紅降解釋放的鐵。以TRF-Fe3+的復(fù)合物形式進(jìn)入骨髓中,供紅的生成
的定向運(yùn)輸和定點(diǎn)的研究已取得了可喜的進(jìn)展. 轉(zhuǎn)鐵蛋白-轉(zhuǎn)鐵蛋白受體在抗和蛋白質(zhì)的定向運(yùn)輸以及作為載體在擴(kuò)散的惡性中的應(yīng)用研究已接近使用階段. 經(jīng)轉(zhuǎn)鐵蛋白-轉(zhuǎn)鐵蛋白受體介導(dǎo)通過血腦屏障進(jìn)入腦內(nèi)發(fā)揮的途徑的研究也給腦的帶來了希望。
Fluorescein labeled transferrin, also called FITC transferrin, is one of fluorescent transferrin conjugates provided by Ruixibio. FITC transferrin is a green fluorescent transferrin conjugate with excitation/emission wavelength at 490 nm/515 nm. Nanocs also provides a number of orange, red, purple, blue and IR dye labeled transferrin products with excitation/emission wavelength. These labeled transferrin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
產(chǎn)品列表:
Transferrin, FITC labeled
Transferrin, Rhodamine labeled
Transferrin, Cy3 labeled
Transferrin, Cy5 labeled
Transferrin, Cy5.5 labeled
Transferrin, Cy7 labeled
Transferrin, Cy7.5 labeled
Transferrin, Biotin labeled
Transferrin, ICG labeled
【熒光標(biāo)記絡(luò)蛋白】:
Ruixibio offers a variety of fluorescently labeled and other functional casein conjugates, which include Fluorescein (FITC) labeled casein, Rhodamine labeled casein, Cy3 labeled casein, Cy5 labeled casein, Cy5.5 labeled casein, Alexa fluor and Biotin labeled casein and many other multifunctional casein products. If you have needs for any special conjugated casein products not listed here, please call us and let we make for you!
Casein, FITC labeled
Casein, Rhodamine labeled
Casein, Cy3 labeled
Casein, Cy5 labeled
Casein, Cy5.5 labeled
Casein, Cy7 labeled
Casein, Cy7.5 labeled
Casein, Biotin labeled
Casein, ICG labeled
【熒光標(biāo)記親和素和鏈霉親和素】:
鏈霉親和素(streptavidin下稱SA)是與親和素(avidin下稱AV)有相似生物學(xué)特性的一種蛋白質(zhì),是streptomyces avidinii菌的分泌物,其分子量及結(jié)合的能力與雞蛋清中的親和素相似,等電點(diǎn)6.0,非特異性結(jié)合遠(yuǎn)比親和素低。
鏈霉親和素是四聚體蛋白,大小為66KDa。一分子鏈霉親和素可以度特異性地與四分子結(jié)合,兩者之間的親和力烈,鏈霉親和素-復(fù)合物的解離常數(shù)處于 10-14 mol/L 數(shù)量級(jí),這一性質(zhì)于分子生物學(xué)用途。其中一條完整的SA肽鏈中有159個(gè)氨基酸殘基,分子量為16450
Avidin is a tetrameric protein commonly isolated from egg whites. Avidin binds to biotin with strength approaching that of a covalent bond. Disruption of the avidin-biotin bond occurs only under extraordinary conditions, such as pH 1.5 with 6 M guanidium hydrocholoride. Fluorescent avidin can bind to biotinylated antibodies used in immunohistochemistry and immunocytochemistry. Avidin thiol can bind to gold surfaces and act as a glue for biotinylated proteins. Avidin agarose is useful for efficient removal of biotinylated proteins from a liquid phase.
產(chǎn)品列表:
Neutravidin-thiol
Streptavidin (without modification)
Streptavidin-CY5
Streptavidin-CY3
Streptavidin-CY5.5
Streptavidin-CY7
Streptavidin-CY7.5
Streptavidin-ICG
Streptavidin-TRITC
Streptavidin-FITC
Streptavidin-thiol
Streptavidin-azide
Streptavidin, HRP labeled
Streptavidin, maleimide activated
Streptavidin, amine function
Streptavidin, DBCO function
Streptavidin, Dextran Conjugate
Streptavidin, R-phycoerythrin
Avidin-CY3
Avidin-CY5
Avidin-CY5.5
Avidin (without modification)
Avidin-Sepharose
Avidin-thiol
Avidin-FITC
Avidin-TRITC
【熒光標(biāo)記和活化改性重組蛋白A】:
Fluorescein labeled Protein A is one of the Ruixibio' premier fluorescent Protein A conjugates. Protein A is a 42 kDa surface protein originally found in the cell wall of Stapylococcus aureus. Protein A has strong binding affinity towards immunoglobulins and it has been used for antibody identification, purification and many other biochemical research uses. Fluorescein protein A is a green fluorescent protein A conjugate that has excitation/emission wavelength at 495 nm/515 nm. All our protein A conjugates were purified by size exclusion chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.
產(chǎn)品介紹:
蛋白A(Protein A)是一種發(fā)現(xiàn)于金黃色葡萄球菌的壁表面蛋白,蛋白G(Protein G)是一種分離自G型或C型鏈球菌屬的表面蛋白,二者功能相似,主要通過與球蛋白(Ig)的Fc區(qū)相互作用,可結(jié)合大多數(shù)哺乳的IgG。然而兩者結(jié)合特異性上有所不同(詳細(xì)見附錄1,蛋白A,G對(duì)不同物種Ig的結(jié)合能力總表),如蛋白G對(duì)人IgG3,大鼠IgG2a,綿羊IgG1具有很的親和力,但蛋白A對(duì)這些Ig結(jié)合力很弱。蛋白A,蛋白G常共價(jià)偶聯(lián)在固體載體如瓊脂糖珠或丙烯酸樹脂小珠上,直接用以做沉淀(IP)或者共沉淀(Co-IP)來進(jìn)行蛋白功能相關(guān)研究,或者直接裝在層析柱上來純化單克隆或者多克隆抗體。
西安瑞禧生物科技有限公司使用的是改造后的蛋白A和蛋白G,不維持其本身的Ig親和特性,同時(shí)也去除了蛋白本身的非主要結(jié)合域以降低非特異性結(jié)合。本品是同時(shí)共價(jià)偶聯(lián)了蛋白A和蛋白G的瓊脂糖珠,比單獨(dú)的蛋白A或者蛋白G都有廣的結(jié)合范圍,適合于蛋白A瓊脂糖珠和蛋白G瓊脂糖珠單獨(dú)可以結(jié)合的Ig,實(shí)用性。本品為50%的懸液,1 ml Protein A/G 瓊脂糖珠可結(jié)合約25 mg人IgG。
FITC-Protein A 異硫氰酸熒光素標(biāo)記蛋白A
Rhodamine-Protein A 羅丹明標(biāo)記蛋白A
CY3-Protein A
CY5-Protein A
CY5.5-Protein A
CY7-Protein A
CY7.5-Protein A
ICG-Protein A
Biotin-Protein A 修飾蛋白A
HRP-Protein A 辣根過氧化物標(biāo)記蛋白A
Protein A, maleimide function 馬來酰亞胺修飾蛋白A
Protein A, thiol function 巰基修飾蛋白A
Protein A, gold labeled 蛋白A修飾納米金
【熒光標(biāo)記和活化改性重組蛋白G】:
Protein G is an immunoglobulin binding protein in Streptococci. Protein G binds to the Fc region of antibodies and thereby leaves the Fab region available to still bind antigen. Our recombinant version has been engineered to lack the albumin binding domains and has a 6x histidine tag at its N-terminus.
FITC-Protein G
Rhodamine-Protein G
CY3-Protein G
CY5-Protein G
CY5.5-Protein G
CY7-Protein G
CY7.5-Protein G
ICG-Protein G
Biotin-Protein G
HRP-Protein G
Protein G, maleimide function
Protein G, thiol function
Protein G, gold labeled
Protein A和 Protein G產(chǎn)品應(yīng)用:
重組蛋白A的C末端含有半胱氨酸,可定向偶聯(lián)到固體分離介質(zhì)上,用于單克隆抗體或多克隆抗體的純化??山Y(jié)合大多數(shù)人類和小鼠球蛋白子類(如。,人類IgG1 IgG2 IgG4;鼠標(biāo)IgG2 IgG2a,IgG2b IgG3),還結(jié)合牛,幾內(nèi)亞豬,倉鼠,房子,豬,兔子總球蛋白的形式。重組蛋白A可與多種報(bào)告分子(熒光染料、標(biāo)記、、膠體金、放射性標(biāo)記等)偶聯(lián)并不影響其與抗體結(jié)合的活性。這些偶聯(lián)后的蛋白A衍生物可用于Western-blot、ELISA、組化等的抗體檢測。
【Green fluorescent protein (GFP)綠色熒光蛋白】:
Green fluorescent protein (GFP) is a 27 kDa protein derived from Aequorea victoria. It absorbs light in the ultraviolet to blue range and emits green (peak emission 510 nm). GFP is a common reporter molecule for protein expression and visualization of intracellular processes. We produce this protein in E.coli.
產(chǎn)品列表:
Green fluorescent protein
GFP-Thiol, Green fluorescent protein, thiol
GFP-Biotin, Green fluorescent protein, Biotin
IgG from human產(chǎn)品:
Human IgG Agarose Beads (Human IgG conjugated to agarose beads)
Human IgG Biotin (Human IgG conjugated to biotin)
Human IgG CY3 (Human IgG conjugated to CY3)
Human IgG CY5 (Human IgG conjugated to CY5)
Human IgG CY5.5 (Human IgG conjugated to CY5.5)
Human IgG FITC (Human IgG conjugated to FITC)
Human IgG (Minimum 95% purity)
IgG from mouse產(chǎn)品:
Mouse IgG CY3 (Mouse IgG conjugated to CY3)
Mouse IgG CY5 (Mouse IgG conjugated to CY5)
Mouse IgG CY5.5 (Mouse IgG conjugated to CY5.5)
Mouse IgG FITC (Mouse IgG conjugated to FITC)
Mouse IgG Agarose Beads (Mouse IgG conjugated to agarose beads)
Mouse IgG Biotin (Mouse IgG conjugated to biotin)
Mouse IgG thiol (Mouse IgG conjugated to thiol)
Mouse IgG HRP (Mouse IgG conjugated to HRP)
Mouse IgG tetramethylrhodamine (Mouse IgG conjugated to tetramethylrhodamine)
Mouse IgG (without modification)
IgG from rabbit產(chǎn)品:
Rabbit IgG CY3 (Rabbit IgG conjugated to CY3)
Rabbit IgG CY5 (Rabbit IgG conjugated to CY5)
Rabbit IgG CY5.5 (Rabbit IgG conjugated to CY5.5)
Rabbit IgG FITC (Rabbit IgG conjugated to FITC)
Rabbit IgG HRP (Rabbit IgG conjugated to HRP)
Rabbit IgG Agarose Beads (Rabbit IgG conjugated to agarose beads)
Rabbit IgG Biotin (Rabbit IgG conjugated to biotin)
Rabbit IgG thiol (Rabbit IgG conjugated to thiol)
Rabbit IgG tetramethylrhodamine (Rabbit IgG conjugated to tetramethylrhodamine)
IgG from rabbit (without modification)
西安瑞禧生物科技有限公司可以提供其他蛋白組學(xué)蛋白交聯(lián)劑部分產(chǎn)品:
BMCC-Biotin
HPDP-Biotin
Iodoacetyl-LC-Biotin
Iodoacetyl-PEG2-Biotin
Maleimide PEG11-Biotin
Maleimide PEG2-Biotin
Amine-PEG3-Biotin
Amine-PEG11-Biotin
Pentylamine-Biotin
Sulfo-NHS-LC-Biotin
NHS-Biotin
NHS-LC-Biotin
NHS-LC-LC-Biotin
NHS-PEG12-Biotin
NHS-SS-Biotin
NHS-SS-PEG4-Biotin
PFP-Biotin
Sulfo-NHS-LC-LC-Biotin
TFP-PEG3-Biotin
Alkoxyamine-PEG12-Biotin
Alkoxyamine-PEG4-Biotin
Alkoxyamine-PEG4-SS-PEG4-Biotin
Hydrazide-Biocytin
Hydrazide-Biotin
Biotin-LC-Hydrazide
Hydrazide-PEG4-Biotin
Psoralen-PEG3-Biotin
TFPA-PEG3-Biotin
Photoactivatable Biotin
NHS-Desthiobiotin
Sulfo-NHS-LC-Desthiobiotin
Amine-PEG4-Desthiobiotin
Hydrazide-PEG4-Desthiobiotin
Sulfo-SHPP
DTSSP
BS3 CAS #: 82436-77-9
BS2G
BSG CAS #: 79642-50-5
DSP CAS #: 57757-57-0
DSS CAS #: 68528-80-3
DTSSP CAS #: 81069-02-5
EGS CAS #: 70539-42-3
Sulfo-EGS
DSSeb CAS # 23024-29-5
BMPS CAS #: 55750-62-4
EMCS CAS #: 55750-63-5
GMBS CAS #: 80307-12-6
LC-SPDP
MBS CAS #: 58626-38-3
PDPH
SBA CAS #: 42014-51-7
SIA CAS #: 39028-27-8
SMCC CAS #: 64987-85-5
SMPB CAS #: 79886-55-8
SMPH
SPDP CAS #: 68181-17-9
Sulfo-LC-SPDP
Sulfo-MBS
Sulfo-SANPAH CAS #: 102568-43-4
Sulfo-SMCC CAS #: 92921-24-9
Biotin Hydrazide CAS #: 66640-86-6
NHS-Biotin CAS #: 35013-72-0
NHS-SS-Biotin CAS #: 142439-92-7
Sulfo-NHS-Biotin
HPG CAS #: 24645-80-5
SATA CAS #: 76931-93-6
Sulfo-NHS Acetate CAS #: 152305-87-8
Biotin-maleimide
Biotin ethylenediamine hydrobromide CAS# 216299-38-6
Biotinamidohexanoic acid hydrazide CAS#: 109276-34-8
CAS#: 89889-52-1
TSAT
BMPH CAS#: 359436-61-6
EMCH CAS#: 151038-94-7
KMUH CAS#: 1593661-78-9
BMOE CAS: 5132-30-9
BMB CAS#: 28537-70-4
BMH (bismaleimidohexane) CAS#:4856-87-5
TMEA
DTME CAS#:71865-37-7
SATP CAS#: 84271-78-3
EMCA CAS: 55750-53-3
Sulfo-NHS,N-羥基琥珀酰亞胺磺酸鈉鹽,CAS #: 106627-54-7
Biotin-ONP,(+)--4-硝基苯酯,CAS#: 33755-53-2
Biotin-PFP,五氟苯酚酯/五氟苯酯,CAS#:120550-35-8
6-(馬來酰亞胺基)己酸琥珀酰亞胺酯,CAS#:55750-63-5
6-Maleimidohexanoic acid,CAS#:55750-53-3
N-Maleoyl-β-alanine(MPA),CAS#:7423-55-4
4-Maleimidobutyric acid(MBA),CAS#:57078-98-5
11-馬來酰亞胺十一烷酸 N-琥珀酰亞胺酯,CAS#:87981-04-2
2-馬來酰亞胺基乙酸,CAS#:25021-08-3
11-Maleimidoundecanoic acid(KMUA)CAS#:57079-01-3
Sulfo-EMCS, ε-馬來酰亞胺己酸磺基琥珀酰亞胺酯 CAS#:215312-86-0
AMAS, CAS#:55750-61-3
1,2-Bis(maleimido)ethane, CAS#:5132-30-9
Boc-6-Ahx-OSu, CAS#:51513-80-5
N-Boc-2,2-(ethylenedioxy)diethylamine ,CAS#:153086-78-3
N,N,N,N-Tetramethyl-p-phenylenediamine, CAS#:100-22-1
N-Succinimidyl 6-Biotinamidohexanoate, CAS#:72040-63-2
N-(+)-Biotinyl-6-aminohexanoic acid CAS#:72040-64-3
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