繼ZFN（Zinc Finger Nucleases）技術(shù)后，Merck在2013年推出新一代基因組編輯工具--CRISPR/Cas9，讓研究人員以更快、更經(jīng)濟(jì)的方式實(shí)現(xiàn)基因組特定位點(diǎn)的編輯。憑借過去10年在基因組編輯領(lǐng)域的豐富經(jīng)驗(yàn)積累以及專業(yè)的生物信息學(xué)平臺(tái)，Merck已經(jīng)成功設(shè)計(jì)出覆蓋人類，小鼠和大鼠三個(gè)物種的所有基因的CRISPR/Cas9載體，并可以提供在線定制服務(wù)，以及完整的CRISPR實(shí)驗(yàn)workflow解決方案。此外，默克與Sanger Institute合作開發(fā)了人、小鼠全基因組CRISPR 文庫，以幫助科學(xué)家實(shí)現(xiàn)基因功能的快速篩選、規(guī)?；哪Ｐ徒⒁约八幬镒饔煤Y選等。

• 高效：優(yōu)化的載體設(shè)計(jì)，大限度提高轉(zhuǎn)染效率，簡化篩選工作
• 特異：特殊的gRNA設(shè)計(jì)和雙切口酶系統(tǒng)，大限度提高特異性
• 全面：產(chǎn)品齊全，可提供質(zhì)粒、RNA、慢病毒載體、RNP等形式，涵蓋人、大鼠、小鼠、植物等多個(gè)物種，更有Sanger Arrayed和Broad Pools全基因組文庫以及重要通路的亞文庫
• 掌控：強(qiáng)大的慢病毒全基因組文庫可輕松進(jìn)行高通量篩選，全面掌控人或小鼠的基因組

CRISPR實(shí)驗(yàn)--載體構(gòu)建和表達(dá)

• CRISPR/Cas gRNA設(shè)計(jì)和選擇 • crRNA定制合成 • 克隆和表達(dá) • 抗生素 • GenElute™質(zhì)粒DNA快速提取kit • PCR擴(kuò)增和核酸電泳 • SP6/T7 體外轉(zhuǎn)錄試劑盒 • GenElute™總RNA提取試劑盒 • 慢病毒包裝系統(tǒng) • 培養(yǎng)皿/離心管/槍頭等基礎(chǔ)耗材

The aim of molecular cloning is to insert the gene-of-interest (GOI) into a plasmid vector. This vector is then inserted into a cell that will express the protein encoded by the GOI. Once protein is expressed, the protein function can be studied as it affects the cell signaling, morphology or other aspects. Alternatively, the protein can be expressed in large quantities that can then be studied directly with other techniques. We have a wide variety of products for use across the cloning & expression workflow.

expression workflow.

Cloning Products

• SnapFast™ Vectors • Cloning Enzymes • Restriction Enzymes • Biomatrica™ Nucleic Acid Stabilization • Custom DNA Oligos

Biomatrica^® offers an innovative alternative to reliance on cold chain logistics

Biomatrica products enable researchers in academia, pharmaceutical drug development, and molecular diagnostics to stabilize ship and store biological samples for long periods of time with complete and rapid sample recovery, all at very affordable costs. Its SampleMatrix^® proprietary core technology stabilizes and protects biological materials at room temperature without degradation.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Energy Efficiency. To see how Stanford incorporated Biomatrica Technology and saved substantial amounts of energy, see their published study:

Biomatrica technology enables safe storage of biological material at room temperature.

Consistent sample maintenance allows for experimental reproducibility, and therefore ensures reliability, speeds time to results, greatly reduces costs, and is an environmentally sustainable alternative to all forms of cold storage. This technology can be applied to thousands of currently available products in the biotechnology, diagnostics and pharmaceutical industries, and has direct applications for billions of samples generated from genome mapping, and sample archiving used for research into personalized medicine and forensics.

		Stabilize and archive gDNA at Room Temperature DNAstable® offers long-term preservation and stabilization of purified gDNA samples at room temperature in a dry-down matrix format. Available in single tubes, well plates (96 & 384) and tube-plates (96). DNAstable® Trial Kit DNAstable® Tube Kit DNAstable® 96-Well Plate DNAstable® Blood, trial kit DNAstable® Blood LD, 10mL bottle DNAstable® Blood LD, 100mL bottle
		Stabilize and archive gDNA at Room Temperature Liquid-based preservative of purified gDNA with optional dry-down for longer storage time. Available as a liquid-based stabilizer in bottles (2ml & 10ml). Highly amenable to automation and high throughput workflows. DNAstable® Plus 2 mL DNAstable® Plus, 10mL bottle
		Collect, Ship and Store Tissues & Cells at Room Temperature DNAgard® offers immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping, processing and storage. The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA. DNAgard® Tissue Trial Kit DNAgard® Tissue 100 mL bottle DNAgard® Blood, 10mL bottle DNAgard® Blood, 50mL bottle DNAgard® Blood, 100mL bottle
		Preserve and Store RNA at Room Temperature RNAstable® offers long-term preservation of purified total RNA, mRNA, miRNA, siRNA and poly(A) RNA samples at room temperature. RNAstable® Trial Kit RNAstable® Tube Kit RNAstable® 96-Well Plate RNAstable® LD (2mL)
		Preserve and Store Bacterial DNA CloneStable® enables the long-term storage of unpurified bacterial plasmid and genomic DNA. DNA recovery is as simple as adding water. CloneStable® Tube Kit

Don’t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available directly through Biomatrica.

Biomatrica^® offers an innovative alternative to reliance on cold chain logistics

Biomatrica products enable researchers in academia, pharmaceutical drug development, and molecular diagnostics to stabilize ship and store biological samples for long periods of time with complete and rapid sample recovery, all at very affordable costs. Its SampleMatrix^® proprietary core technology stabilizes and protects biological materials at room temperature without degradation.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Energy Efficiency. To see how Stanford incorporated Biomatrica Technology and saved substantial amounts of energy, see their published study:

Biomatrica technology enables safe storage of biological material at room temperature.

Consistent sample maintenance allows for experimental reproducibility, and therefore ensures reliability, speeds time to results, greatly reduces costs, and is an environmentally sustainable alternative to all forms of cold storage. This technology can be applied to thousands of currently available products in the biotechnology, diagnostics and pharmaceutical industries, and has direct applications for billions of samples generated from genome mapping, and sample archiving used for research into personalized medicine and forensics.

		Stabilize and archive gDNA at Room Temperature DNAstable® offers long-term preservation and stabilization of purified gDNA samples at room temperature in a dry-down matrix format. Available in single tubes, well plates (96 & 384) and tube-plates (96). DNAstable® Trial Kit DNAstable® Tube Kit DNAstable® 96-Well Plate DNAstable® Blood, trial kit DNAstable® Blood LD, 10mL bottle DNAstable® Blood LD, 100mL bottle
		Stabilize and archive gDNA at Room Temperature Liquid-based preservative of purified gDNA with optional dry-down for longer storage time. Available as a liquid-based stabilizer in bottles (2ml & 10ml). Highly amenable to automation and high throughput workflows. DNAstable® Plus 2 mL DNAstable® Plus, 10mL bottle
		Collect, Ship and Store Tissues & Cells at Room Temperature DNAgard® offers immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping, processing and storage. The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA. DNAgard® Tissue Trial Kit DNAgard® Tissue 100 mL bottle DNAgard® Blood, 10mL bottle DNAgard® Blood, 50mL bottle DNAgard® Blood, 100mL bottle
		Preserve and Store RNA at Room Temperature RNAstable® offers long-term preservation of purified total RNA, mRNA, miRNA, siRNA and poly(A) RNA samples at room temperature. RNAstable® Trial Kit RNAstable® Tube Kit RNAstable® 96-Well Plate RNAstable® LD (2mL)
		Preserve and Store Bacterial DNA CloneStable® enables the long-term storage of unpurified bacterial plasmid and genomic DNA. DNA recovery is as simple as adding water. CloneStable® Tube Kit

Don’t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available directly through Biomatrica.