Gene Bridges是一家依托歐洲分子生物技術(shù)實(shí)驗(yàn)室(EMBL),成立于2000年6月的高科技生物科技公司,該公司擁有的技術(shù)Red/ET基因重組技術(shù)可實(shí)現(xiàn)DNA任意位點(diǎn)的克隆/亞克隆,以及精準(zhǔn)的克隆出如BACs或大腸桿菌染色體的超長(zhǎng)NDA分子,已經(jīng)為多個(gè)制藥企業(yè)、生物技術(shù)公司及科研院所提供大量的基因克隆及超過500個(gè)已結(jié)題的DNA修飾項(xiàng)目。
Gene Bridges公司的專業(yè)團(tuán)隊(duì)擁有嫻熟的技術(shù)人員及深悉分子及微生物技術(shù)、小鼠基因組學(xué)、轉(zhuǎn)基因技術(shù)、生物化學(xué)、代謝工程及工業(yè)化的資深科學(xué)家。力求為生命科學(xué)研究領(lǐng)域及產(chǎn)業(yè)化提供優(yōu)質(zhì)的真核/原核基因重組、表達(dá)產(chǎn)品與服務(wù)。
Gene Bridges公司主要產(chǎn)品:
一、Red/ET試劑盒:該系列產(chǎn)品包括:
(1)Quick & Easy BAC(細(xì)菌人工染色體)修飾試劑盒,用于所有類型的BCA基因修飾。
(2)Counter Selection BAC Modification Kit:使用counter-selection cassette用于高級(jí)的BAC及細(xì)菌染色體的修飾。
(3)BAC Subcloning Kit
用于BAC質(zhì)粒DNA段的亞克隆。
(4)Quick & Easy Conditional Knock Out Kit - FRT
用于簡(jiǎn)便快速的FRT位點(diǎn)整合入大的質(zhì)粒載體的任意需要位點(diǎn)(適用于轉(zhuǎn)基因小鼠的模型制作)
(5)Quick & Easy Conditional Knock Out Kit - loxP
用于簡(jiǎn)便快速的loxP位點(diǎn)整合入大的質(zhì)粒載體的任意需要位點(diǎn)(適用于轉(zhuǎn)基因小鼠的模型制作)
(6)Quick & Easy E. coli Gene Deletion Kit
用于E. coli染色體上基因的快速敲出或修飾。
(7)E. coli strain GB05-dir
用于直接克隆E. coli的linear plus linear 同源重組。
(8)E. coli strain GB08-red
用于基因重組E. coli 的 linear plus circular同源重組。
二、Red/ET重組的篩選盒(selection cassettes)該系列產(chǎn)品包括:
(1)PGK-gb2-neo:原核/真核生物+新霉素篩選盒
(2)FRT-PGK-gb2-neo-FRT:原核/真核生物+新霉素+ FRT位點(diǎn)篩選盒
(3)loxP-PGK-gb2-neo-loxP:原核/真核生物+新霉素+loxP 位點(diǎn)篩選盒
(4)FRT-PGK-gb2-neo-FRT-loxP:原核/真核生物+新霉素+ FRT位點(diǎn)+下游loxP 位點(diǎn)篩選盒
(5)loxP-FRT-PGK-gb2-neo-FRT:原核/真核生物+新霉素+ FRT位點(diǎn)+上游loxP 位點(diǎn)篩選盒
(6)FRT-gb2-cm-FRT:原核生物+氯霉素+ FRT位點(diǎn)篩選盒
(7)loxP-gb2-cm-loxP:原核生物+氯霉素+ loxP位點(diǎn)篩選盒
(8)FRT-gb2-amp-FRT:原核生物+氨芐青霉素+ FRT位點(diǎn)篩選盒
(9)loxP-gb2-amp-loxP:原核生物+氨芐青霉素+ loxP位點(diǎn)篩選盒
(10)FRT-PGK-gb2-hygro-FRT:原核/真核生物+潮霉素+ FRT位點(diǎn)篩選盒
(11)loxP-PGK-gb2-hygro-loxP:原核/真核生物+潮霉素+ loxP位點(diǎn)篩選盒
(12)iCre-FRT-neo-FRT:原核/真核生物+改良密碼子的Cre (iCre) +新霉素+ 前后FRT位點(diǎn)篩選盒
(13)iCreERT2-FRT-neo-FRT:原核/真核生物+配體誘導(dǎo)的Cre (iCre)+ 前后FRT位點(diǎn)篩選盒T
三、重組的表達(dá)質(zhì)粒:該系列產(chǎn)品有多款表達(dá)性質(zhì)粒,包括:
(1)707-FLPe, tet:抗四環(huán)素的Flp表達(dá)質(zhì)粒(僅用于E. coli)
(2)707-FLPe, cm:抗氯霉素的Flp表達(dá)質(zhì)粒(僅用于E. coli)(3)707-FLPe, amp:抗氨芐青霉素的Flp表達(dá)質(zhì)粒(僅用于E. coli)(4)710-FLPe, km:抗卡那霉素的Flp表達(dá)質(zhì)粒(僅用于E. coli)
(5)705-Cre, cm:抗氯霉素的Cre表達(dá)質(zhì)粒(僅用于E. coli)
(6)706-Cre, tet:抗四環(huán)素的Cre表達(dá)質(zhì)粒(僅用于E. coli)(7)704-Cre,amp:抗氨芐青霉素的Cre表達(dá)質(zhì)粒(僅用于E. coli)
(8)pCAGGS-FLPe (科研/工業(yè)化):增強(qiáng)的真核生物FLP表達(dá)質(zhì)粒(適用于37°C)
(9)pCAGGS-FLPo:真核生物FLP重組酶表達(dá)質(zhì)粒,其重組酶密碼子配對(duì)被改良以促進(jìn)其在哺乳動(dòng)物細(xì)胞內(nèi)表達(dá)。(工業(yè)化生產(chǎn)需Fred Hutchinson腫瘤研究中心授權(quán)批準(zhǔn))
(10)pCAGGS-Cre:真核生物Cre表達(dá)質(zhì)粒
(11)pCAGGS-Dre:真核生物Dre重組酶表達(dá)質(zhì)粒。(工業(yè)化生產(chǎn)需Stowers醫(yī)學(xué)研究所授權(quán)批準(zhǔn))
(12)pSC101-BAD-Cre: Arabinose誘導(dǎo)原核生物Cre重組酶表達(dá)質(zhì)粒。
(13)pSC101-BAD-Dre :Arabinose誘導(dǎo)原核生物Dre重組酶表達(dá)質(zhì)粒。(工業(yè)化生產(chǎn)需Stowers醫(yī)學(xué)研究所授權(quán)批準(zhǔn))
(14)pSC101-BAD-FLPe:Arabinose誘導(dǎo)原核生物FLP重組酶表達(dá)質(zhì)粒。
貨號(hào) | 品名 | 包裝 | 價(jià)格 | 品牌 |
K004 | Quick & Easy Red/ET Conditional Knock Out Kit - FRT (need license to buy) | Kit | 16146 | genebridges |
K005 | Quick & Easy Red/ET Conditional Knock Out Kit - loxP (need license to buy) | Kit | 16146 | genebridges |
K006 | Quick & Easy Red/ET E. coli Gene Deletion Kit (need license to buy) | Kit | 13806 | |
A008 | Red/ET Ampicillin Selection Cassette, FRT-flanked (FRT-amp-FRT) | 50ng/ul,20ul | 6435 | |
A009 | Red/ET Ampicillin Selection Cassette, loxP flanked (loxP-amp-loxP) | 50ng/ul,20ul | 6435 | |
K003 | Red/ET BAC Subcloning Kit (need license to buy) | Kit | 14040 | |
A006 | Red/ET Chloramphenicol Selection Cassette, FRT-flanked (FRT-cm-FRT) | 50ng/ul,20ul | 6435 | |
A007 | Red/ET Chloramphenicol Selection Cassette, loxP-flanked (loxP-cm-loxP) | 50ng/ul,20ul | 6435 | |
K002 | Red/ET Counter Selection BAC Modification Kit (need license to buy) | Kit | 18486 | |
A112 | Red/ET Cre Expression Plasmid:705-Cre, cm-selection | 0.2ug/ul,20ul | 6435 | |
A113 | Red/ET Cre Expression Plasmid:706-Cre, tet-selection | 0.2ug/ul,20ul | 6435 | |
A201 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academic price, requires MTA) | 0.2ug/ul,20ul | 8775 | |
A202 | Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA) | 0.2ug/ul,20ul | 76050 | |
A104 | Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection | 0.2ug/ul,20ul | 6435 | |
A105 | Red/ET Enhanced Flp Expression Plasmid:708-FLPe, cm-selection | 0.2ug/ul,20ul | 6435 | |
A010 | Red/ET Hygromycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-hygro-FRT) | 50ng/ul,20ul | 6435 | |
A011 | Red/ET Hygromycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-hygro-loxP) | 50ng/ul,20ul | 6435 | |
A005 | Red/ET Neomycin Selection Cassette plus loxP site 2nd version, FRT flanked, Prokaryotic and Eukaryotic (loxP-FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6435 | |
A004 | Red/ET Neomycin Selection Cassette plus loxP site, FRT flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT-loxP) | 50ng/ul,20ul | 6435 | |
A002 | Red/ET Neomycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-neo-FRT) | 50ng/ul,20ul | 6435 | |
A003 | Red/ET Neomycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-neo-loxP) | 50ng/ul,20ul | 6435 | |
A001 | Red/ET Neomycin Selection Cassette, Prokaryotic and Eukaryotic (PGK-gb2-neo) | 50ng/ul,20ul | 6435 | |
K001 | Red/ET Quick & Easy BAC Modification Kit (need license to buy) | Kit | 15687 |
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