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詳細(xì)介紹
Lumegen型號體外三維血管應(yīng)力加載培養(yǎng)系統(tǒng)(Pulsatile Pressure & Flow Bioreactor) 是一種全新設(shè)計的血管體外應(yīng)力培養(yǎng)裝置。該系統(tǒng)為體外完整的血管器官提供搏動、壓應(yīng)力培養(yǎng)刺激三維機(jī)械力刺激培養(yǎng)環(huán)境，可長時間進(jìn)行體外血管應(yīng)力刺激三維培養(yǎng)，使血管存活較長時間，可分別調(diào)節(jié)壓力與切應(yīng)力大小，控制定常流與脈動流等血液動環(huán)境．為血管生物力學(xué)和血管組織工程學(xué)的研究提供一種更有效的實(shí)驗(yàn)手段。
系統(tǒng)亮點(diǎn)：
1. 培養(yǎng)腔重量輕，結(jié)構(gòu)緊湊，耐腐蝕可聯(lián)用二氧化碳培養(yǎng)箱進(jìn)行長期培養(yǎng)
2. 培養(yǎng)腔室可進(jìn)行高壓滅菌
3. 多通道灌注動力泵，可通行運(yùn)行6個腔室
4. lumegen 200mmHg搏動刺激和壓力傳感器保證了血管力值
5. 培養(yǎng)腔容積只有60毫升,節(jié)省了大量的營養(yǎng)液與昂貴的生長因子
6. 用戶可定義的zui符合生理?xiàng)l件幅度的正弦壓力波
7. 計算機(jī)控制貫流泵可以調(diào)節(jié)搏動和壓力的大小
8. Growthworks軟件控制的計算機(jī)系統(tǒng)可以實(shí)時繪制波形圖
9. 聚碳酸酯窗戶可拆卸，便于查看和激光測量千分尺
腔室兩側(cè)有透明的可移動的窗口，可進(jìn)行視覺監(jiān)測
10. 培養(yǎng)腔室可更換不同的夾具，以適應(yīng)不同的樣品直徑
（內(nèi)直徑:2～6mm）
11. 多波形控制，數(shù)據(jù)采集和多電機(jī)運(yùn)行
我們公司專注細(xì)胞力學(xué)和3D細(xì)胞打印代理銷售與實(shí)驗(yàn)技術(shù)服務(wù)，隨帶給您發(fā)一下細(xì)胞組織力學(xué)一些其他科研產(chǎn)品，包括：
1.美國Flexcell細(xì)胞應(yīng)力加載培養(yǎng)系統(tǒng)包括：
1.1.FX-5000T體外細(xì)胞拉應(yīng)力加載培養(yǎng)系統(tǒng)（tension system）
1.2.FX-5000C細(xì)胞組織壓應(yīng)力加載培養(yǎng)系統(tǒng)（compression system）
1.3.FX-5000TT細(xì)胞組織三維拉應(yīng)力加載培養(yǎng)系統(tǒng)（Tissue train culture system）
1.4.STR-4000流體剪切應(yīng)力加載刺激系統(tǒng)（FlexFlow™ and Streamer® Fluid Shear Stress Systems）
1.1、1.2、1.3 三系統(tǒng)可以共用一套電腦軟件系統(tǒng)，組合一個大平臺。
各系統(tǒng)介紹請見，http://www.bioexcellenceint。。ｃｏｍ/flexcell.html 網(wǎng)頁。
2、德國Rszelltechnik 單細(xì)胞應(yīng)力加載、形變測量與力特性??分析系統(tǒng)
3、美國ActiveLife品牌 活體骨組織參考點(diǎn)壓痕測試分析系統(tǒng)
4、加拿大AuroraScientific肌肉機(jī)械力測試分析系統(tǒng)
5、美國Tissue growth品牌體外三維組織（血管、軟骨、骨組織、心臟膜瓣、皮膚）應(yīng)力加載培養(yǎng)系統(tǒng)
6、瑞士regenhu品牌3D細(xì)胞生物打印系統(tǒng)
7、意大利SKE 品牌與德國Minucell&minutissue 品牌細(xì)胞組織多通道三維灌流培養(yǎng)系統(tǒng)
我們可以按照您需求定制科研工具設(shè)備，：.
LumeGen & CardioGen: Pulsatile Pressure & Flow Bioreactor System
BISS's series of bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The patented LumeGen & CardioGen systems (US Patent # 7348175) impart pulsatile flow and pressure to a vascular conduit. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices.
LumeGen Chambers
The LumeGen bioreactor chamber creates pulsatile and shear stress stimulation for a single vascular construct. Standard construct sizes may range from 2 to 6mm inner diameter and from 10 to 60mm in length. The modular design facilitates scaffold installation and cell seeding separate from the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further instrumentation. The autoclavable chamber has a volume of 60mL. Additional chamber features include:
Design:Polycarbonate removable windows for easy viewing and laser micrometer measurementSize:90mm(3.6”)L x 46mm(1.6”)W x 20mm(0.8”)DPorts:5
LumeGen Grip Options
The LumeGen chambers use barbed stainless steel fittings with an elastic clamp to immobilize the construct. Standard barb sizes accommodate .8, 1, 1.5, 2, 3, 4, 5 and 6mm lumen internal diameters and are interchangeable using a universal connector system.
CardioGen Chambers
The CardioGen bioreactor chamber accommodates pulsatile and shear stress stimulation for a single valve construct. Standard construct sizes may range from 9 to 21 mm inner diameter and from 50 to 80 mm long. Grip placement can accommodate both pulmonary valves with straight downstream vessels and aortic valves with angled downstream vessels. The modular design facilitates scaffold installation and cell seeding separate from the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further instrumentation. The autoclavable chamber has a volume of 430 mL. Additional chamber features include:
Design:Polycarbonate hinged windows for easy viewing and laser micrometer measurementSize:145mm(5.7”)L x 105mm(4.15”)W x 74mm(2.9”)DPorts:6
CardioGen Grip Options
CardioGen chambers use barbed stainless steel fittings and either sutures or clamps to fix the construct in place. Standard barb sizes range in size from 9 to 21 mm and are interchangeable using a universal connector system.
Bioreactor Stimulator:
The LumeGen bioreactor system includes the 6Vr pulsatile pressure mechanical stimulator. The 6Vr stimulator features a 40N linear motor that is compact, corrosion resistant and incubator compatible. The stimulator employs pressure control to deliver user defined sinusoidal or physiological pressure waveforms to a maximum pressure of 200 mmHg. The 6Vr is integrated with a computer controlled mean flow pump and is capable of low or high-flow configurations. The flow rate of the mean flow pump is automatically altered to maintain the desired mean pressure within the heart valve. The stimulator dimensions are as follows:
Size:152mm(6.0”)H x 102mm(4.0”)W x 102mm(4.0”)DWeight:2.1kg (4.6lb)
GrowthWorks Software & Control:
The GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows? laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.
The GrowthWorks Control Hardware features an embedded In CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.
GrowthApps Stiffness Measurement & Adaptive Control:
The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the tendon or ligament from initial seeding to an implantation-ready state with minimal operator intervention.
LumeGen Bioreactor Configurations
LumeGen bioreactorc configurations range from a single chamber to an integrated six chamber system. The 1V system is a single chamber design that is able to support basic pulsatile pressure and shear stress experiments. Optional features can be added including laser micrometers, pressure sensors and other transducers. The LumeGen 6Vc system integrates up to six chambers,individual reservoirs, pumps and stimulator onto a frame that can be easily placed into an incubator. Individual flow loops are provided for each chamber. The table below describes the features of the 1V and 6Vc systems.
VASCULARKey: S=Standard, A=Available, - = not availableCHAMBERS1V6VcIndividual vascular growth chamberSS6 parallel vascular growth chambers-SSample grips for 3, 4, 5, 6 mm lumen diameterSSExternal lumen instrumentation ports per chamber33Internal lumen instrumentation ports per chamber22O-ring style sample fixationAAPatent pending grip lock during sample handlingSSLumen/ablumen volume isolators/reducers for scaffold seedingAA
STIMULATION Static pressure control (200 mm Hg peak pressure)AAPulsatile pressure control (sinusoidal)ASPulsatile pressure control (physiological content, user defined waveform)ASAxial distension stimulationAA
CONTROLS Controller software, computer, and monitorSSPower supplies and cablingSSMean cell culture media flow controlASDynamic cell culture media flow controlAS
TISSUE MONITORING Non-contact radial or axial distension measurementAANon-contact flow sensorsAACatheter pressure transducersAA
ACCESSORIES Media reservoirSSIncubatorAA
BENEFITS All-autoclavable componentsSSExcellent sample visibilitySSMedia change without opening reservoir via luer fittingsSSEasy rotisserie cell seeding owing to cylindrical chamber frameSSLight weight design for easy handlingSSTransparent opposing bioreactor walls for optical inspection or measurementSSCompact design to fit in standard incubatorsSSEasily cleaned surface finishesSS100% satisfaction guaranteed warrantySS
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