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ImageStreamX MarkII Amnis 量化成像分析流式細(xì)胞儀介紹
將流式的統(tǒng)計(jì)學(xué)力量和熒光顯微鏡的形態(tài)學(xué)內(nèi)涵深度結(jié)合,讓您突破“散點(diǎn)圖”的單一數(shù)據(jù)模式,在傳統(tǒng)流式熒光信號強(qiáng)度參數(shù)基礎(chǔ)上,為您提供超過百種量化成像參數(shù)用以進(jìn)行不同細(xì)胞群體的分析。
● 下一代的“先鋒級”流式細(xì)胞儀,流式發(fā)展*具有里程碑意義的劃時(shí)代產(chǎn)品;
● 先鋒級硬件配置,多7根激光器,5MESF極限級熒光靈敏度,好級分辨率;
● 真正的多參數(shù)分析,提供每個(gè)細(xì)胞超過100種量化參數(shù),獲得更全面、更深入的流式數(shù)據(jù);
● 除了熒光強(qiáng)度單一參數(shù)以外,還提供準(zhǔn)確的影像學(xué)判定,一次可實(shí)現(xiàn)數(shù)萬乃至數(shù)十萬細(xì)胞的高內(nèi)涵數(shù)據(jù)分析結(jié)果;
● 眾多*應(yīng)用,細(xì)胞間相互作用,蛋白定位,內(nèi)吞,核轉(zhuǎn)位等等,將流式細(xì)胞技術(shù)從單一表型分析工具拉升到個(gè)體和群體功能性研究的綜合性平臺。
ImageStreamX MarkII Amnis 量化成像分析流式細(xì)胞儀應(yīng)用
ImageStreamX MarkII參數(shù)
● 激光器:標(biāo)配為488、642和785nm激光器,可拓展375、405、561、592、642nm激光器
● 檢測通道:標(biāo)配為6個(gè)檢測通道,包括1個(gè)明場、1個(gè)暗場和至多5個(gè)熒光檢測通道;可升級為12個(gè)檢測通道,包括2個(gè)明場、1個(gè)暗場(側(cè)向角散射)和至多10個(gè)熒光檢測通
● 物鏡:標(biāo)配為40倍,可升級為20/40/60倍物鏡組
● 熒光靈敏度:小于5MESF
● 上樣體積:20-20 μl
Amnis ImageStreamX MarkII introduction:
The ImageStreamX is Amnis' second generation imaging flow cytometer and the result of over 10 years of development. The raw power of the ImageStreamX for cell analysis is unmatched: it produces up to 12 high resolution images of each cell directly in flow, at rates exceeding 1,000 cells per second, and with the fluorescence sensitivity of the best conventional flow cytometers. These breakthrough capabilities allow you to quantitate cellular morphology and the intensity and location of fluorescent probes on, in, or between cells, even in rare sub-populations and highly heterogeneous samples.
Though ImageStream technology is years ahead of anything else, it has been thoroughly proven. Statistically robust and objective ImageStream data have been published in over 150 peer-reviewed articles to date. By combining the speed, sensitivity, and phenotyping abilities of conventional flow cytometry with the detailed imagery and functional insights of microscopy, think of how the ImageStreamX will advance your research.
With the ImageStreamX you can:
● Image cells directly in suspension with the resolution of a 60X microscope and the fluorescence sensitivity of the best flow cytometers.
● Analyze highly heterogeneous samples and rare cell sub-populations at speeds exceeding 1,000 cells per second.
● Perform phenotypic and functional studies at the same time using up to five lasers and 12 images per cell.
● Quantitate virtually anything you can see using the IDEAS® software package's numerous pre-defined fluorescence and morphologic parameters.
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