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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫>>人正常細(xì)胞>>CCL-25WISH 細(xì)胞,人羊膜細(xì)胞

          WISH 細(xì)胞,人羊膜細(xì)胞

          參   考   價(jià): ¥ 1

          訂  貨  量: ≥1 件

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          產(chǎn)品型號:CCL-25

          品       牌:其他品牌

          廠商性質(zhì):生產(chǎn)商

          所  在  地:上海市

          更新時(shí)間:2024-11-16 16:45:40瀏覽次數(shù):1490

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          供貨周期 一周 貨號 WISH
          應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥 主要用途 科學(xué)研究
          WISH 細(xì)胞,人羊膜細(xì)胞
          原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

          WISH 細(xì)胞,人羊膜細(xì)胞

          ATCC® Number:CCL-25™    Price:$323.00
          Additional information about this cell line

          Designations:WISH

          Depositors:L Hayflick

          Biosafety Level:2

          Shipped:frozen

          Medium & Serum:See Propagation

          Growth Properties:adherent

          Organism:Homo sapiens (human)

          Morphology:epithelial


          Source:Organ: HeLa contaminant

          Cellular Products:keratin

          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



          Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)

          Reverse Transcript:negative

          DNA Profile (STR):Amelogenin: X

          CSF1PO: 9,10

          D13S317: 13.3

          D16S539: 9,10

          D5S818: 11,12

          D7S820: 8,12

          THO1: 7

          TPOX: 8,12

          vWA: 16,18



          Isoenzymes:G6PD, A

          HeLa Markers:Y

          WISH 細(xì)胞,人羊膜細(xì)胞



          Comments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.

          The cells are positive for keratin by immunoperoxidase staining.

          NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination



          Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
          Temperature: 37.0°C


          Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended
          Medium Renewal: Twice per week

          Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.



          Preservation:culture medium 95%; DMSO, 5%

          Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

          recommended serum:ATCC 30-2020



          References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed:13712490
          26034: . . Am. J. Dis. Child. 103: 460, 1962.





















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