您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
您好, 歡迎來到化工儀器網(wǎng)！ 登錄| 免費注冊| 產(chǎn)品展廳| 收藏商鋪|
18906011628
當(dāng)前位置：> 供求商機> PDGF BB Human, Yeast /重組人血小板源生長因子-BB,酵母
| Synonyms | Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Plaet-derived growth factor beta polypeptide, Becaplermin. |
| Introduction | PDGF-BB is a member of the plaet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer （PDGF-BB） or as a heterodimer with the plaet-derived growth factor alpha polypeptide （PDGF-AB）, where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. |
| Description | Plaet-Derived Growth Factor BB Human Recombinant is a homodimeric, glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 32,021 Dalton. PDGF-BB is purified by proprietary chromatographic techniques. |
| Source | Saccharomyces cerevisiae. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized from 0.2 μm filtered solution in 20mM sodium acetate in absence of any carrier protein. |
| Solubility | It is recommended to reconstitute the lyophilized Plaet-derived Growth Factor-BB in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Plaet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
| Purity | Greater than 97.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu. |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells （measured by 3H-thymidine uptake） is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg. |
| Protein content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
請輸入賬號
請輸入密碼
請輸驗證碼
以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負責(zé)，化工儀器網(wǎng)對此不承擔(dān)任何保證責(zé)任。
溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。
