黄色视频不卡_午夜福利免费观看在线_亚洲国产精品999在线_欧美绝顶高潮抽搐喷水_久久精品成人免费网站_晚上一个人看的免费电影_国产又色又爽无遮挡免费看_成人国产av品久久久

    1. <dd id="lgp98"></dd>
      • <dd id="lgp98"></dd>
        1. 北京西美杰科技有限公司

          主營產(chǎn)品: 生物學檢測試劑盒,化學試劑,蛋白質(zhì)氧化損傷,抗原抗體

          15

          聯(lián)系電話

          18210960361

          您現(xiàn)在的位置: 首頁> 技術(shù)文章 > 安全高效的蛋白結(jié)晶條件篩選試劑盒

          公司信息

          聯(lián)人:
          西美杰
          話:
          400-050-4006
          機:
          18210960361
          售后電話:
          010-88597838
          址:
          北京市海淀區(qū)金源時代商務(wù)中心B座10F
          編:
          100097
          網(wǎng)址:
          www.xmjsci.com
          鋪:
          http://facexiu.com/st118010/
          給他留言

          安全高效的蛋白結(jié)晶條件篩選試劑盒

          2019-12-6  閱讀(734)

              想要獲得高質(zhì)量蛋白晶體一般需要克3個難題1、獲得純度高的,均一的蛋白樣品;2,篩選可結(jié)晶的條件;3,優(yōu)化結(jié)晶條件,后獲得高質(zhì)量的單晶;其中,篩選可結(jié)晶的條件是獲得蛋白質(zhì)晶體的主要瓶頸之一;

              西美杰代理的Jena Biosource品牌中有一系列蛋白結(jié)晶研究所要用的試劑和耗材,以下給大家介紹一下Jena里面的蛋白結(jié)晶條件篩選試劑盒。

          1、JBScreen Basic

          JBScreen Basic是基于稀疏矩陣法的蛋白結(jié)晶試劑盒,包含了96種篩選條件,整套試劑盒分為4個小規(guī)格,每個小規(guī)格包含24種條件,24個條件分別密封在螺旋蓋試管中,每管10ml,可以隨時使用。Jena里面的蛋白結(jié)晶篩選試劑盒不包含二甲shenjia

          鹽,用MES進行替代。

           

          名稱

          貨號

          規(guī)格

          JBScreen Basic 1

          CS-121

          24 solutions (10 ml each)

          JBScreen Basic 2

          CS-122

          24 solutions (10 ml each)

          JBScreen Basic 3

          CS-123

          24 solutions (10 ml each)

          JBScreen Basic 4

          CS-124

          24 solutions (10 ml each)

          JBScreen Basic 1 – 4

          CS-125

          4 Kits

          JBScreen Basic HTS

          CS-203L

          96 solutions (1.7 ml each)

           

          2、 JBScreen Classic

                這個系列是Jena品牌中初推出的經(jīng)典的結(jié)晶條件試劑盒,在文獻中的應(yīng)用以每年30%的速度增長;這個試劑盒包含了240個篩選條件,涵蓋了各種有效沉淀劑和Buffer。整套試劑盒分成了10小規(guī)格,每個規(guī)格包含了24個條件,如果只想要其中某些條件,是可以單獨購買小規(guī)格試劑盒的;

           

          名稱

          貨號

          條件數(shù)量

          JBScreen Classic 1 (PEG 400 to 3000 based)

          CS-101L

          24 solutions (10 ml each)

          JBScreen Classic 2 (PEG 4000 based)

          CS-102L

          24 solutions (10 ml each)

          JBScreen Classic 3 (PEG 4000+ based)

          CS-103L

          24 solutions (10 ml each)

          JBScreen Classic 4 (PEG 5000 MME to 8000 based)

          CS-104L

          24 solutions (10 ml each)

          JBScreen Classic 5 (PEG 8000 to 20000 based)

          CS-105L

          24 solutions (10 ml each)

          JBScreen Classic 6 (Ammonium Sulfate based)

          CS-106L

          24 solutions (10 ml each)

          JBScreen Classic 7 (MPD based)

          CS-107L

          24 solutions (10 ml each)

          JBScreen Classic 8 (MPD/Alcohol based)

          CS-108L

          24 solutions (10 ml each)

          JBScreen Classic 9 (Alcohol/Salt based)

          CS-109L

          24 solutions (10 ml each)

          JBScreen Classic 10 (Salt based)

          CS-110L

          24 solutions (10 ml each)

           

          JBScreen Classic 1–10

          CS-114L

          10 Kits

          JBScreen Classic HTS I (PEG based)

          CS-201L

          96 solutions (1.7 ml each)

          JBScreen Classic HTS II (Ammonium Sulfate, MPD, Alcohol and Salt based)

          CS-202L

          96 solutions (1.7 ml each)

           

           

                   3、JBScreen pentaerythritol

                基于兩種新奇的沉淀劑,Pentaerythrotol丙氧基化物和乙基化物,用于生物大分子初結(jié)晶條件的篩選。兩者都包含一個Pentaerythrotol的支鏈型高分子。因此他們不同于傳統(tǒng)的沉淀劑(如MPD和PEG)。另外,Pentaerythrotol合物具有冷凍保護劑的功能,蛋白晶體在這些高濃度的沉淀劑中生長,并能夠由晶滴直接冷凍;

              

          名稱

          貨號

          規(guī)格

          JBScreen Pentaerythritol 1 (PEP 426 based)

          CS-191

          24 solutions (10 ml each)

          JBScreen Pentaerythritol 2 (PEP 629 based)

          CS-192

          24 solutions (10 ml each)

          JBScreen Pentaerythritol 3 (PEE 270 based)

          CS-193

          24 solutions (10 ml each)

          JBScreen Pentaerythritol 4 (PEE 797 based)

          CS-194

          24 solutions (10 ml each)

          JBScreen Pentaerythritol 1 – 4

          CS-195

          4 Kits

          JBScreen Pentaerythritol HTS

          CS-210L

          96 solutions (1.7 ml each)

           

               參考文獻:

          1. Sheu-Gruttadauria et al. (2019) Beyond the seed: structural basis for supplementary microRNA targeting by human Argonaute2. The EMBO Journal e101153.
          2. Pozzi et al. (2019) Evidence of Destabilization of the Human Thymidylate Synthase (hTS) Dimeric Structure Induced by the Interface Mutation Q62R. Biomolecules DOI:10.3390/biom9040134.
          3. Deka et al. (2018) Structural and biochemical studies on the role of active site Thr166 and Asp236 in the catalytic function of D-Serine deaminase from Salmonella typhimurium. Biochem. Biophys. Res. Commun. 504:40.
          4. Dall et al. (2018) Structural and functional analysis of cystatin E reveals enzymologically relevant dimer and amyloid fibril states. J. Biol. Chem. 293:13151.
          5. Rinaldi et al. (2018) Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state. Biochem. Biophys. Rep. 16:39.
          6. Flores-Ibarra et al. (2018) Crystallization of a human galectin-3 variant with two ordered segments in the shortened N-terminal tail. Sci. Rep. 8:9835.
          7. Bernedo-Navarro et al. (2018) Structural Basis for the Specific Neutralization of Stx2a with a Camelid Single Domain Antibody Fragment. Toxins 10:108.
          8. Zeng et al. (2017) Structural basis of host recognition and biofilm formation by Salmonella Saf pili. eLife DOI:10.7554/eLife.28619.
          9. Oiki et al. (2017) Alternative substrate-bound conformation of bacterial solute-binding protein involved in the import of mammalian host glycosaminoglycans. Sci. Rep. 7:17005.
          10. Jansson et al. (2017) The interleukin-like epithelial-mesenchymal transition inducer ILEI exhibits a non-interleukin-like fold and is active as a domain-swapped dimer. J. Biol. Chem. 292:15501.
          11. McPhail et al. (2017) The Molecular Basis of Aichi Virus 3A Protein Activation of Phosphatidylinositol 4 Kinase IIIβ, PI4KB, through ACBD3. Structure 25:121.
          12. Songsiriritthigul et al. (2017) Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori. Acta Cryst F 73:62.
          13. Yokoyama et al. (2017) Large-scale crystallization and neutron crystallographic analysis of HSP70 in complex with ADP. Acta Cryst F 73:555.
          14. Corvaglia et al. (2019) Carboxylate-functionalized foldamer inhibitors of HIV-1 integrase and Topoisomerase 1: artificialanalogues of DNA mimic proteins. Nucleic Acids Research DOI:10.1093/nar/gkz352.
          15. Deka et al. (2017) Comparative structural and enzymatic studies on Salmonella typhimurium diaminopropionate ammonia lyase reveal its unique features. J. Struct. Biol. DOI:10.1016/j.jsb.2017.12.012.
          16. Moonens et al. (2015) Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies. Veterinary Research 46:14.
          17. Zano et al. (2014) Structure of an unusual S-adenosylmethionine synthetase from Campylobacter jejuni. Acta Cryst. D 70:442.
          18. Goyal et al. (2013) Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG. Acta Cryst. F69:1349.
          19. Fujita et al. (2017) Structural Flexibility of an Inhibitor Overcomes Drug Resistance Mutations in Staphylococcus aureus FtsZ. ACS Chem. Biol. 12:1947.
          20. Weidenweber et al. (2017) Structure of the acetophenone carboxylase core complex: prototype of a new class of ATP-dependent carboxylases/hydrolases. Sci. Rep. 7:39674.
          21. Fujita et al. (2017) Identification of the key interactions in structural transition pathway of FtsZ from Staphylococcus aureus. J. Struct. Biol. 198:65.
          22. Wagner et al. (2016) The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters. Science 354:114.
          23. Demmer et al. (2015) Insights into Flavin-based Electron Bifurcation via the NADH-dependent Reduced Ferredoxin:NADP Oxidoreductase Structure. JBC 290:21985.
          24. Rekittke et al. (2015) Structure of the GcpE-HMBPP complex from Thermus thermophilius. Biochem. Biophys. Res. Commun.458:246.
          25. Uchida et al. (2014) Structure and properties of the C-terminal β-helical domain of VgrG protein from Escherichia coli O157. J. Biochem. 155(3):173.
          26. McDougall et al. (2019) Proteinaceous Nano container Encapsulate Polycyclic Aromatic Hydrocarbons. Sci. Rep. 9:1058.
          27. De Wijn et al. (2018) Combining crystallogenesis methods to produce diffraction-quality crystals of a psychrophilic tRNA-maturation enzyme. Acta Cryst F 74:747.
          28. Kumar et al. (2018) Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase. Int. J. Biol. Macromol. 117:890.
          29. Leal et al. (2018) Crystal structure of DlyL, a mannose-specific lectin from Dioclea lasiophylla Mart. Ex Benth seeds that display cytotoxic effects against C6 glioma cells. Int. J. Biol. Macromol. 114:64.
          30. Sousa Cavada et al. (2018) Canavalia bonariensis lectin: Molecular bases of glycoconjugates interaction and antiglioma potential. Int. J. Biol. Macromolec. 106:369.
          31. Ernst et al. (2018) A comparative structural analysis of the surface properties of asco-laccases. PLOS ONEDOI:10.1371/journal.pone.0206589.
          32. Kumar et al. (2017) Non-classical transpeptidases yield insight into new antibacterials. Nat. Chem. Biol. 13:54.
          33. Nascimento et al. (2017) Structural analysis of Dioclea lasiocarpa lectin: A C6 cells apoptosis-inducing protein. Int. J. Biochem. Cell Biol. 92:79.
          34. Cattani et al. (2015) Structure of a PEGylated protein reveals a highly porous double-helical assembly. Nat. Chem. 7:823.
          35. Boltsis et al. (2014) Non-contact Current Transfer Induces the Formation and Improves the X?ray Diffraction Quality of Protein Crystals. Crystal Growth & Design 14:4347.
          36. Kampatsikas et al. (2017) In crystallo activity tests with latent apple tyrosinase and two mutants reveal the importance of the mutated sites for polyphenol oxidase activity. Acta Cryst. F 73:491.
          37. Kolek et al. (2016) A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase. Acta Cryst. F 72:307.
          38. Tan et al. (2014) A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa. Acta Cryst. D 70:2054.
          39. Li et al. (2014) Crystallizing Membrane Proteins in the Lipidic Mesophase. Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy. Crystal Growth & Design 14:2034.
          40. Jacobs et al. (2012) Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris. Acta Cryst. F 68:1255.
          41. Li et al.(2011) Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen. Crystal Growth & Design 11(2):530.
          42. Shaw Stewart et al. (2011) Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization. Crystal Growth & Design 11(8):3432.
          43. Caffrey et al. (2009) Crystallizing Membrane Proteins Using Lipidic Mesophases. Nat Protoc. 4:706.
          44. Cherezov et al. (2006) In Meso Structure of the Cobalamin Transporter, BtuB, at 1.95 Å Resolution. J. Mol. Biol. 364:716.


          產(chǎn)品對比 產(chǎn)品對比 二維碼 在線交流

          掃一掃訪問手機商鋪

          對比框

          在線留言
          新绛县| 石棉县| 曲水县| 浪卡子县| 前郭尔| 麦盖提县| 木里| 开化县| 柞水县| 玉屏| 通榆县| 乌恰县| 临西县| 孙吴县| 万州区| 泸西县| 宁波市| 灵台县| 娱乐| 东港市| 无棣县| 赣州市| 安岳县| 陵水| 石楼县| 定襄县| 湟源县| 梁河县| 东莞市| 辉南县| 霍山县| 托克逊县| 象州县| 福安市| 绥宁县| 印江| 民乐县| 保山市| 曲阜市| 泰和县| 固阳县|