北京西美杰科技有限公司
主營產(chǎn)品: 生物學檢測試劑盒,化學試劑,蛋白質(zhì)氧化損傷,抗原抗體 |
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安全高效的蛋白結(jié)晶條件篩選試劑盒
2019-12-6 閱讀(734)
想要獲得高質(zhì)量蛋白晶體一般需要克服3個難題:1、獲得純度高的,均一的蛋白樣品;2,篩選可結(jié)晶的條件;3,優(yōu)化結(jié)晶條件,后獲得高質(zhì)量的單晶;其中,篩選可結(jié)晶的條件是獲得蛋白質(zhì)晶體的主要瓶頸之一;
西美杰代理的Jena Biosource品牌中有一系列蛋白結(jié)晶研究所要用的試劑和耗材,以下給大家介紹一下Jena里面的蛋白結(jié)晶條件篩選試劑盒。
1、JBScreen Basic
JBScreen Basic是基于稀疏矩陣法的蛋白結(jié)晶試劑盒,包含了96種篩選條件,整套試劑盒分為4個小規(guī)格,每個小規(guī)格包含24種條件,24個條件分別密封在螺旋蓋試管中,每管10ml,可以隨時使用。Jena里面的蛋白結(jié)晶篩選試劑盒不包含二甲shenjia
鹽,用MES進行替代。
名稱 | 貨號 | 規(guī)格 |
---|---|---|
JBScreen Basic 1 | CS-121 | 24 solutions (10 ml each) |
JBScreen Basic 2 | CS-122 | 24 solutions (10 ml each) |
JBScreen Basic 3 | CS-123 | 24 solutions (10 ml each) |
JBScreen Basic 4 | CS-124 | 24 solutions (10 ml each) |
JBScreen Basic 1 – 4 | CS-125 | 4 Kits |
JBScreen Basic HTS | CS-203L | 96 solutions (1.7 ml each) |
2、 JBScreen Classic
這個系列是Jena品牌中初推出的經(jīng)典的結(jié)晶條件試劑盒,在文獻中的應(yīng)用以每年30%的速度增長;這個試劑盒包含了240個篩選條件,涵蓋了各種有效沉淀劑和Buffer。整套試劑盒分成了10小規(guī)格,每個規(guī)格包含了24個條件,如果只想要其中某些條件,是可以單獨購買小規(guī)格試劑盒的;
名稱 | 貨號 | 條件數(shù)量 |
---|---|---|
JBScreen Classic 1 (PEG 400 to 3000 based) | CS-101L | 24 solutions (10 ml each) |
JBScreen Classic 2 (PEG 4000 based) | CS-102L | 24 solutions (10 ml each) |
JBScreen Classic 3 (PEG 4000+ based) | CS-103L | 24 solutions (10 ml each) |
JBScreen Classic 4 (PEG 5000 MME to 8000 based) | CS-104L | 24 solutions (10 ml each) |
JBScreen Classic 5 (PEG 8000 to 20000 based) | CS-105L | 24 solutions (10 ml each) |
JBScreen Classic 6 (Ammonium Sulfate based) | CS-106L | 24 solutions (10 ml each) |
JBScreen Classic 7 (MPD based) | CS-107L | 24 solutions (10 ml each) |
JBScreen Classic 8 (MPD/Alcohol based) | CS-108L | 24 solutions (10 ml each) |
JBScreen Classic 9 (Alcohol/Salt based) | CS-109L | 24 solutions (10 ml each) |
JBScreen Classic 10 (Salt based) | CS-110L | 24 solutions (10 ml each)
|
JBScreen Classic 1–10 | CS-114L | 10 Kits |
JBScreen Classic HTS I (PEG based) | CS-201L | 96 solutions (1.7 ml each) |
JBScreen Classic HTS II (Ammonium Sulfate, MPD, Alcohol and Salt based) | CS-202L | 96 solutions (1.7 ml each) |
3、JBScreen pentaerythritol
基于兩種新奇的沉淀劑,Pentaerythrotol丙氧基化物和乙基化物,用于生物大分子初結(jié)晶條件的篩選。兩者都包含一個Pentaerythrotol的支鏈型高分子。因此他們不同于傳統(tǒng)的沉淀劑(如MPD和PEG)。另外,Pentaerythrotol合物具有冷凍保護劑的功能,蛋白晶體在這些高濃度的沉淀劑中生長,并能夠由晶滴直接冷凍;
名稱 | 貨號 | 規(guī)格 |
---|---|---|
JBScreen Pentaerythritol 1 (PEP 426 based) | CS-191 | 24 solutions (10 ml each) |
JBScreen Pentaerythritol 2 (PEP 629 based) | CS-192 | 24 solutions (10 ml each) |
JBScreen Pentaerythritol 3 (PEE 270 based) | CS-193 | 24 solutions (10 ml each) |
JBScreen Pentaerythritol 4 (PEE 797 based) | CS-194 | 24 solutions (10 ml each) |
JBScreen Pentaerythritol 1 – 4 | CS-195 | 4 Kits |
JBScreen Pentaerythritol HTS | CS-210L | 96 solutions (1.7 ml each) |
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