目錄:北京博蕾德生物科技有限公司>>其他>>切片相關(guān)>> HitobiotecGolgi-Cox OptimStain Prekit 高爾基染色
供貨周期 | 現(xiàn)貨 | 規(guī)格 | Standard Kit |
---|---|---|---|
貨號 | HTKNS1125NH | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
主要用途 | 研究神經(jīng)元和神經(jīng)膠質(zhì)細(xì)胞形態(tài) |
Hitobiotec Golgi-Cox OptimStain™ Prekit 高爾基染色試劑盒
關(guān)鍵詞
高爾基染色試劑盒,高爾基,Golgi Staining,Hitobiotec Corp.;Golgi-Cox impregnation 浸染法;neuron 神經(jīng)元(神經(jīng)細(xì)胞);glia 神經(jīng)膠質(zhì)(膠質(zhì)細(xì)胞)
產(chǎn)品描述
Golgi-Cox 染色或Golgi-Cox 浸染法(Golgi-Cox impregnation)認(rèn)為是用來研究神經(jīng)元和神經(jīng)膠質(zhì)細(xì)胞形態(tài)的有效和美觀的通用方法之一。近些年高爾基體染色Golgi-Cox 染色法依然是用來檢查樹突分枝模式和樹突棘的優(yōu)選方法,因其能對大腦某特定區(qū)域隨機挑取的少數(shù)片段內(nèi)的樹突樹(dendritic arbour)進行分離和鑒定。
高爾基染色技術(shù)不僅只用于解剖研究,而且廣泛用于行為-形態(tài)關(guān)系研究。一些研究中Golgi-Cox法曾用來鑒定和研究心臟自主神經(jīng)支配,結(jié)果顯示Golgi-Cox法是一種非常有效的技術(shù)來鑒定和研究外周血組織自主神經(jīng)支配的形態(tài)學(xué)特征。然而,Golgi-Cox染色法很費時,需要使用振動切片機,細(xì)胞染色率通常比較低,結(jié)果常常不可靠。
Hitobiotec Corp.公司生產(chǎn)的高爾基體染色試劑盒(Hito Golgi-Cox OptimStain™ Kit)針對以上問題提供了一種簡單的解決方案?;?span>Glaser and Van der Loos描述的方法,本試劑盒對Golgi-Cox 技術(shù)做出了顯著改善。此試劑盒步驟簡單且操作時間明顯降低??蒲腥藛T可使用-19oC保存的冷凍切片機(cryostat)。不僅提供穩(wěn)定和改善的染色質(zhì)量,而且只要適當(dāng)操作幾乎無過度染色。標(biāo)準(zhǔn)試劑盒可用于50個小鼠腦切片染色。
如果沒有冷凍切片機(cryostat),組織可用薄片切片機(microtome)制備成石蠟切片。
Hitobiotec Corp.公司生產(chǎn)的高爾基體染色試劑盒(Hito Golgi-Cox OptimStain™ Kit)經(jīng)測試廣泛用于不同動物的腦、脊髓和心臟組織,以及高靈敏度顯示神經(jīng)元和神經(jīng)膠質(zhì)細(xì)胞的形態(tài)學(xué)細(xì)節(jié)。
Golgi-Cox OptimStain Prekit 高爾基染色
試劑盒組分
組分 | Standard Kit | Small Kit |
Solution-1A (Stock Solution) 溶液-1A | 100 ml | 50 ml |
Solution-1B (Prepare Solution) 溶液-1B | 150 ml | 75 ml |
Solution-2 溶液2 | 250 ml | 125 ml |
Solution-3 溶液3 | 500 ml | 250 ml |
Solution-4 溶液4 | 250 ml | 125 ml |
Solution-5 溶液5 | 250 ml | 125 ml |
Dropper Bottle (30 ml Solution-3) 滴瓶 | 1 | 1 |
Staining Jar (12 ml) 染缸 | 2 | 1 |
Fine Tip Natural Hair Brush | 2 | 1 |
Glass Specimen Transfer Tool | 2 | 1 |
User Manual and MSDS | 1 | 1 |
染色結(jié)果示例:
品牌 | 名稱 | 貨號 | 備注 |
Hitobiotec | Hito Golgi-Cox OptimStain PreKit | HTKNS1125NH | 安全運輸版(不含有毒試劑) |
注意事項:
完整版的Hito Golgi-Cox Optimstain kit內(nèi)含有毒試劑:Hgcl2和重鉻-酸鉀,受到出口限制,運輸費用和手續(xù)較復(fù)雜。
我們推薦客戶訂購:不含有毒試劑的:Hito Golgi-Cox OptimStain PreKit,這個是針對國際客戶的去掉了Hgcl2和重鉻-酸鉀的安全運輸版,貨到后,我們添加了在Solution1溶液瓶里加入Hgcl2和重鉻-酸鉀5.25克,添加后和完整版的是一樣的,這個試劑盒是無害的,可以用普通包裝郵寄。
引用文獻:
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Golgi-Cox OptimStain Prekit 高爾基染色
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