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參考價(jià)	￥ 1312
訂貨量	≥1

Chondrex

稀釋液

DSS

ELISA

試劑盒

糖苷

膠原試劑盒

動(dòng)物模型

佐劑

組織染色

蛋白分析

膠原

抗體檢測(cè)盒

抗體

kit collagen mg

咨詢與訂購(gòu)：北京博蕾德科技發(fā)展有限公司

Nephritogenic NC1 Fragment

Glomerulonephritis can be induced in WKY rats by immunization with the non-collagenous domain （NC1） of type IV collagen, which is the major component of glomerular basement membranes （GBM）, as well as by a single injection of monoclonal antibodies against alpha 4 （IV） NC1. Chondrex provides the NC1 fraction of type IV collagen isolated from bovine GBM for inducing nephritis in rats. In addition, NC1 fragments purified from mouse and rat renal type IV collagen are supplied for studying autoantibodies to GBM in mice and rats.

NC1 Fragment of Type IV Collagen

Product	Quantity	Catalog #
NC1 Fragment of Bovine GBM Type IV Collagen	1 mg, lyophilized	1102
NC1 Fragment of Mouse Renal Type IV Collagen	0.1 mg, lyophilized	1106
NC1 Fragment of Rat Renal Type IV Collagen	0.1 mg, lyophilized	1104

Induction of Glomerulonephritis in WKY/NHsd Rats （Harlan） by Immunization with the NC1 Fragment of Bovine Type IV Collagen
Female WKY/NHsd rats （Harlan） were immunized with 50 µg of the NC1 fragment of bovine type IV collagen from glomerular basement membrane （GBM） emulsified with CFA （M. tuberculosis, 1mg/ml, catalog # 7008） by subcutaneous injection at the base of the tail on day 0 and observed for the of a nephritogenic monoclonal antibody, Clone b35. The severity of nephritis was determined by the total amount of proteins secreted in a 16-hour urine collection （from 5:00 PM to 9:00 AM）. Rats immunized with the NC1 fraction of bovine GBM type IV collagen, developed mild nephritis with medium proteinuria on day 10, and lasted until day 28. Similarly, the control rats, which received b35, developed mild nephritis within 5 days and lasted at least 14 days. These results indicated that WKY/NHsd strain of rats from Harlan are medium responders to glomerulonephritis induced by immunization with the NC1 of type IV collagen and by IP injection of monoclonal antibodies to the NC1 of type IV collagen.

NOTE: For unknown reasons, mice are generally resistant to GBM autoantibody-mediated nephritis.

Figure 1 - Induction of glomerular nephritis in WKY/NHsd （Harlan, USA） by immunization with the NC1 fragment of bovine GBM type IV collagen

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ELISA grade bovine type I collagen

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