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GE Pharmacia 17-0851-01 預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns
- 公司名稱 上海嘉適科學(xué)儀器有限公司
- 品牌
- 型號 GE Pharmacia 17-0851-01
- 產(chǎn)地
- 廠商性質(zhì) 經(jīng)銷商
- 更新時間 2018/5/14 9:00:00
- 訪問次數(shù) 7473
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預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns
GE預(yù)裝脫鹽柱 Disposable PD-10 Desalting Columns IMPROVED：一次性脫鹽緩沖液交換制備。 球蛋白分離范圍：1,000-5,000 柱體積：8.3ml
Technical Information
PD-10 columns are packaged with a convenient column stand¾the PD-10 Desalting Workmate. Use LabMate Buffer Reservoir for easier equilibration.
Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol (order separay).
Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
| TECHNICAL SPECIFICATIONS | |
| Disposable PD-10 Desalting Columns | |
| Matrix | Sephadex G-25 Medium |
| Separation mechanism | According to size |
| Particle size range | 85-260 µm |
| Exclusion limit | Mr 5000 |
| Sample volume (gravity) | 1.0 to 2.5 ml |
| Sample volume (spin) | 1.75 to 2.5 ml |
| Column volume | 13.5 ml |
| Medium volume | 8.3 ml |
| Column material | Polypropylene barrel and polyethylene frits |
| Chemical stability | All commonly used buffers |
| Working pH range | 2¾13 |
| Storage temperature | 4°C to 30°C |
Removal of NaCl from BSA using the gravity protocol. Recovery of the sample was 95% and the desalting capacity was > 99%.
PD-10 Desalting Columns give fast desalting with gravity flow or spin protocols. The blue adapters are used together with the spin protocols.
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